geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Mutation analysis of the C-terminal vacuolar targeting peptide of tobacco chitinase: low specificity of the sorting system, and gradual transition between intracellular retention and secretion into the extracellular space


, : Mutation analysis of the C-terminal vacuolar targeting peptide of tobacco chitinase: low specificity of the sorting system, and gradual transition between intracellular retention and secretion into the extracellular space. Plant Journal 5(1): 45-54

The C-terminal propeptide of tobacco (Nicotiana tabacum) chitinase A has been shown to be necessary and sufficient for targeting of chitinases to the plant vacuole. The sequence specificity of this vacuolar targeting peptide (VTP) has now been analysed using transient expression of chitinases in Nicotiana plumbaginifolia protoplasts. An extracellular cucumber chitinase, previously used as a secreted reporter protein in transgenic tobacco, was also secreted into the incubation medium by the transiently transformed protoplasts. Addition of six to seven amino acids at the C-terminus to generate the VTP of tobacco chitinase A were sufficient to cause retention of most of the cucumber chitinase within the protoplasts. The chitinase A itself, as well as a mutant lacking the N-terminal chitin-binding domain, were retained to 80% in the protoplasts when low concentrations of the plasmid were used in the transient expression system. At high concentrations of plasmid, causing high levels of transiently expressed chitinase, retention was reduced, indicating saturation of the sorting system. Deletion of the C-terminal methionine did not affect the intracellular location, but deletion of even a single internal amino acid of the VTP caused predominantly secretion of tobacco chitinase A. In contrast, exchanges of amino acids in the VTP as well as substitution of the VTP with random sequences had intermediary effects that covered the whole range from retention to secretion. The results suggest that the sorting system responsible for the diversion of secretory proteins to the vacuole has a low specificity for the sequence of C-terminal targeting peptides, and that sequence changes in the VTP allow a gradual transition from vacuolar retention to secretion.

US$19.90

PMID: 8130797

DOI: 10.1046/j.1365-313x.1994.5010045.x


Other references

Pannu, P.P.S.; Chahal, S.S.; Mandeep Kaur; Sidhu, S.S., 2005: Influence of weather variables on the development of brown leaf spot caused by Helminthosporium oryzae in rice. The effect of different weather parameters (minimum and maximum temperature, relative humidity and rainfall) on the development of brown leaf spot (caused by H. oryzae [Cochliobolus miyabeanus]) in rice cv. PR-116 (susceptible) was investigated un...

P.C.K.ndalia; G.C. Chennaiah, 1978: Spatial analysis of landuse over Idukki District using remote sensing techniques. Journal of the Indian Society of Remote Sensing 6(2): 75-78

Beckmann, C.Anderson., 2002: A descriptive study of women's perceptions of their asthma during pregnancy. To determine the impact of asthma during pregnancy from the perspective of the pregnant woman. A descriptive survey using the Internet for subject recruitment and data collection was conducted using an adaptation of the Cancer Survivors Survey Que...

Woszczyk, J., 1974: The phenomenon of oblique frequency distribution as exemplified by blood clotting factors. A neglected problem. Polski Tygodnik Lekarski 29(40): 1693-1696

Terry, C.W., 1948: Some 1947 results of barn hay drying research. Some cost figures on mow hay drying are given. The avg. cost per ton was $1.70. A number of factors influence the heat of respiration. Data on the effect of rel. humidity, air flow, kind of hay, and temp. are given. The removal of water in unit qu...

Wahlström, H.; Andersson, Y.; Plym-Forshell, L.; Pires, S.M., 2011: Source attribution of human Salmonella cases in Sweden. The aim of this study was to identify the sources of sporadic domestic Salmonella cases in Sweden and to evaluate the usefulness of a source-attribution model in a country in which food animals are virtually free from Salmonella. The model allocat...

Permyakov, E.A.; Medvedkin, V.N.; Mitin, Y.V.; Kretsinger, R.H., 1991: Noncovalent complex between domain AB and domains CD*EF of parvalbumin. The interaction between domain AB and domains CD*EF of pike parvalbumin III has been studied by intrinsic fluorescence spectroscopy. In the presence of Ca2+ ions, parvalbumin fragment 38-108 containing two calcium binding sites interacts with the...

Panchamukhi B.G.; Mudholkar D.R., 1973: Study of the arteries and veins angio architecture of the internal genitalia of female goats with particular reference to the ovaries. Indian Journal of Animal Sciences 43(12): 1057-1060

Popoff S.N.; Schneider G.B., 1985: Processing of concanavalin a ligand complexes by osteoclasts in vitro. Anatomical Record 211(3): 155A

Aryana, K., J.; Mcgrew, P., 2007: Quality attributes of yogurt with Lactobacillus casei and various prebiotics. The objective was to determine the effect of chain length of inulins on the characteristics of fat-free plain yogurt manufactured with Lactobacillus casei. Probiotic fat-free plain yogurts were manufactured using Streptococcus thermophilus, Lacto...