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Oxidant-mediated ciliary dysfunction in human respiratory epithelium

, : Oxidant-mediated ciliary dysfunction in human respiratory epithelium. Free Radical Biology & Medicine. 17(1): 1-10

Exposure of human nasal ciliated epithelium to reactive oxidants generated by the enzymatic xanthine-xanthine oxidase superoxide/hydrogen peroxide (H-2O-2) and glucose-glucose oxidase H-2O-2-generating systems, or to reagent H-2O-2 or hypochlorous acid (HOCl) resulted in significant alterations in ciliary beating. The earliest change noted was the presence of ciliary slowing, progressing eventually to complete ciliary stasis in some areas. Ciliary dyskinesia was seen within the first hour, often from as early as 15 min after exposure of the cells to reactive oxidants. Using peroxidases, various antioxidant enzymes, and oxidant scavengers, we confirmed that these detrimental effects on ciliary function were mediated primarily by H-2O-2 and HOCl. Moreover, 3-aminobenzamide (3-ABA), an inhibitor of the DNA repair enzyme poly ADP ribose polymerase, prevented H-2O-2-mediated inhibition of ciliary function, indicating that oxidant-mediated damage to DNA may well be the basis of the effects of H-2O-2 on ciliated epithelium. Acute and chronic inflammatory responses may therefore present the possible threat of H-2O-2- or HOCl-inflicted injury on bystander respiratory epithelium, leading to ciliary dyskinesia and slowing.


PMID: 7959161

DOI: 10.1016/0891-5849(94)90002-7

Other references

Goulliarr, M., 1953: Observations biologiques et recherches sur le pigment respiratior chez Pamphipode Urothoe grimaldi (Chevreux). Bulletin de la Societe Zoologique de France, 77: 388-394

Gavagnin, M.; Mollo, E.; Castelluccio, F.; Ghiselin, M.T.; Calado, G.; Cimino, G., 2001: Can molluscs biosynthesize typical sponge metabolites? The case of the nudibranch Doriopsilla areolata. The first study on the biosynthesis of terpenoid metabolites in the porostome nudibranch Doriopsilla areolata is described herein. The de novo origin of two different groups of bi- and tri-cyclic sesquiterpenes, exhibiting opposite A/B ring juncti...

Ban, S.; Hasegawa, J.; Anusavice, K.J., 1992: Effect of loading conditions on bi-axial flexure strength of dental cements. A ball-on-three-ball bi-axial flexure test was used to evaluate the flexure strengths of five kinds of dental cements (zinc phosphate, polycarboxylate, glass ionomer, silicate, and zinc oxide-eugenol cements). The bi-axial flexure strengths of zin...

Lawrence, J.M.; Liese, A.D.; Liu, L.; Dabelea, D.; Anderson, A.; Imperatore, G.; Bell, R., 2008: Weight-loss practices and weight-related issues among youth with type 1 or type 2 diabetes. OBJECTIVE - The purpose of this study was to describe the weight-loss practices and weight-related issues reported by youth with diabetes, according to sex and diabetes type. RESEARCH DESIGN AND METHODS - A total of 1,742 female and 1,615 male you...

Ammar, H.O.; Ghorab, M.; El-Nahhas, S.A.; Higazy, I.M., 2011: Proniosomes as a carrier system for transdermal delivery of tenoxicam. Tenoxicam is a non steroidal anti-inflammatory drug (NSAID) widely used in the treatment of rheumatic diseases and characterized by its good efficacy and less side effects compared to other NSAIDs. Its oral administration is associated with severe...

Lukaszewicz-Hussain, A.; Moniuszko-Jakoniuk, J.; Rogalska, J., 2007: Assessment of lipid peroxidation in rat tissues in subacute chlorfenvinphos administration. Objective: To study the levels of malonyldialdehyde, marked as the concentration of substances reacting with thiobarbituric acid in liver, brain, serum and kidney in subacute intoxication with low doses of chlorfenvinphos, an organophosphate inse...

Nordenram, A.; Björnesjö, K.B., 1970: Allotransplantation of teeth in man on the basis of the Normal Lymphocyte Transfer (NLT) test. Odontologisk Revy 21(2): 169-188

Ohbayashi, F.; Suzuki, M.G.; Shimada, T., 2002: Sex determination in Bombyx mori. We describe here the current status and future prospects of sex determination studies in the silkworm, B. mori. The sex of Bombyx is strongly controlled by the presence of the W chromosome. Although several classical studies suggested that a presu...

Noguchi, Y., 1990: Japan's land problem. Land is a factor of production and an asset. The total value of land in Japan was the equivalent of 55% of the country's national wealth in 1985. Of this, 80% was attributed to residential land and 12.5% to farmland. With regard to ownership,...

Johnson C.E.; Debaz B.P.; Shurin P.A.; Marchant C.D.; Shah Z.R.; Murdell Panek D.; Scillian J.J.; Debartolomeo R.M., 1984: Radiologic evaluation of pediatric urinary infections localized to kidney or bladder. Radiology 153(SPEC ED): 266