geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

PDGF isoform-induced proliferation and receptor expression in human cultured airway smooth muscle cells


, : PDGF isoform-induced proliferation and receptor expression in human cultured airway smooth muscle cells. American Journal of Physiology 270(3 Pt 1): L415-L428

The effect of recombinant platelet-derived growth factor (PDGF) isoforms (PDGF-AA, -BB and -AB) on mitogenesis of human cultured airway smooth muscle (ASM) was examined using the MTT-reduction assay and [3H]thymidine incorporation. Results were correlated with expression of PDGF receptor (PDGFR)-alpha and -beta subunits in the absence and presence of fetal calf serum (FCS). When FCS was absent PDGF-AB and -BB were potent mitogens, whereas PDGF-AA was weakly mitogenic, evoking <20% of the maximum response induced by the B-chain isoforms. When FCS (2.5%) was present, all PDGF isoforms stimulated marked ASM proliferation with similar efficacy and potency. Cross-competition binding analysis in FCS-deprived cells revealed that ASM cells in culture express mainly PDGFR-beta. Preincubation with PDGF-AA or PDGFR-alpha neutralizing antiserum abolished PDGF-AA binding and decreased total receptor number by approximately 15%. The ratio of PDGFR-alpha to beta subunits was approximately 1:8, supported by intense immunofluorescence staining for PDGFR-beta and weak staining for PDGFR-alpha. In parallel studies, uptake of [3H]thymidine stimulated by PDGF-AA, but not PDGF-AB or -BB, was inhibited by PDGFR-alpha immobilization. Western immunoblot analysis confirmed expression of mature PDGFR-alpha and -beta subunits in ASM cells. FCS did not cause any detectable increase in PDGFR-alpha expression or in PDGF-AA binding. These data support a role for PDGFR-beta mediating ASM mitogenesis during FCS-free conditions, but in the presence of FCS, both PDGFR-alpha and -beta subunits are linked to mitogenesis. The enhanced mitogenicity of PDGF-AA in the presence of FCS was independent of any detectable upregulation of PDGFR-alpha, suggesting that the inability of PDGF-AA to promote mitogenesis in the absence of FCS is not simply due to relative numbers of PDGFR-alpha and PDGFR-beta.

US$29.90

PMID: 8638734


Other references

Pizer, E.S.; Kurman, R.J.; Pasternack, G.R.; Kuhajda, F.P., 1997: Expression of fatty acid synthase is closely linked to proliferation and stromal decidualization in cycling endometrium. Estrogen-driven proliferative phase growth is the most rapid physiological proliferative process that occurs in the adult. The tissue growth that occurs during this phase of the menstrual cycle requires incorporation of a substantial quantity of f...

Holla, B.S.; Shivananda, M.K.; Akberali, P.M.; Baliga, S.; Safeer, S., 1996: Studies on arylfuran derivatives-Part VI. Synthesis, characterization and antibacterial activities of some 6-(5-aryl-2-furyl)-1,2,4-triazolo [3,4-b]-1,3,4-thiadiazoles and 6-(5-nitro-2-furyl)-1,2,4-triazolo[3,4-b]-1,3,4-thiadiazoles. A series of 4-[5-aryl-2-furfurylidene]amino-3-mercapto-5-substituted-1,2,4-tri azoles and 4-[5-Nitro-2-furfurylidene]amino-3-mercapto-5-substituted-1,2,4-tr iazoles have been synthesized and were converted into 1,2,4-triazolo [3,4-b]-1,3,4-thiadia...

Umitbayev R.B.; Sindeyev A.S.; Zhupakhin Y.N., 1968: Prospecting for epithermal gold deposits in the northeastern USSR. Gold-bearing quartz veins, connected with subvolcanic acidic intrusions, independent of hydrothermally altered zones, late Cretaceous-early Tertiary, Okhotsk-Chukchi volcanic belt.

Cleary, M.F., 1977: Fluphenazine decanoate during pregnancy. American Journal of Psychiatry 134(7): 815-816

Miccoli, P.; Biricotti, M.; Matteucci, V.; Ambrosini, C.E.; Wu, J.; Materazzi, G., 2015: Minimally invasive video-assisted thyroidectomy: reflections after more than 2400 cases performed. The minimally invasive video-assisted approach was developed for primary hyperparathyroidism in 1997 and the year after for thyroid disease. Since then, the technique has been adopted worldwide, and indications moved from the initial benign diseas...

Katz, J.S.; Doh, J.; Irvine, D.J., 2005: Composition-tunable properties of amphiphilic comb copolymers containing protected methacrylic acid groups for multicomponent protein patterning. Methods to micropattern multiple protein components on surfaces under mild conditions are of interest for biosensing, proteomics, and fundamental studies in cell biology. Here, we report on the composition-dependent thin-film solubility behavior o...

Baer, G.M.; Shanthaveerappa, T.R.; Bourne, G.H., 1965: Studies on the pathogenesis of fixed rabies virus in rats. Investigations were made on the spread of fixed rabies virus after its inoculation into the rear foot-pads of rats. The presence of rabies virus in the central nervous system was first detected in the lumbar segment of the spinal cord. Removal of...

Cotta, C.V.; Zhang, Z.; Kim, H-Gyoon.; Klug, C.A., 2003: Pax5 determines B- versus T-cell fate and does not block early myeloid-lineage development. Progenitor B cells deficient in Pax5 are developmentally multipotent, suggesting that Pax5 is necessary to maintain commitment to the B-cell lineage. Commitment may be mediated, in part, by Pax5 repression of myeloid-specific genes. To determine w...

Stemwedel, J.D., 2003: "Causes" in chemical explanations. Chemists talk about causes and make frequent use of the verb "to cause." They use the verb "to cause" for its generality in identifying causal chains, whether all the details of those chains are established or some remain uncle...

Modic, P., 1980: The significance and role of proteins of animal and plant origin in the manufacture of meat products. Non-meat proteins are allowed in sausage emulsions in a majority of West European countries, the USA and Canada; the permissible amount is as much as 50% in some States. At present, the proteins used with meat products are: (i) animal proteins fro...