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PH-Dependent molecular weight changes of kappa-casein glycomacropeptide and its preparation by ultrafiltration


, : PH-Dependent molecular weight changes of kappa-casein glycomacropeptide and its preparation by ultrafiltration. Milchwissenschaft 48(4): 191-196

K-Casein glycomacropeptide (GMP) is a biologically active peptide released from K-casein by chymosin. Although the theoretical molecular weight (MW) of GMP was about 7 kDa, the apparent MW of GMP on gel filtration was changed depending on pH. The distribution of the apparent MW ranged from 20 to 50 kDa at pH 7, but from 10 to 30 kDa at pH 3.5. GMP was rejected at pH 6.5 with ultrafiltration (UF) membranes with MW cut off of 50 or 20 kDa, but was mostly permeated through the same UF membranes at pH 3.5. We developed a simple procedure using UF for isolating GMP from whey protein concentrate (WPC), taking the pH-dependent change in the apaprent MW into account. The WPC solution was subjected to UF and diafiltration under acidic conditions using UF membrane with MW cut off of 50 kDa. After the permeate was neutralized, the second UF with the same UF membrane was carried out to retain GMP in the retentate. A lyophilized GMP powder was recovered with 63% yield and 81% purity.

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Other references

Gängel, H.; Stumpf, J., 1968: Lameness in long type breeding pigs. Monatshefte für Veterinarmedizin 23(19): 731-737

Weinstein, I., 1962: Anastomosis of a severed parotid duct: report of case. Journal of Oral Surgery, Anesthesia, and Hospital Dental Service 20: 156-158

DePuey, E.G.; Port, S.; Wackers, F.J.; Rozanski, A.; Botvinick, E.H.; Dae, M.W.; Tamaki, N., 1998: Nonperfusion applications in nuclear cardiology: report of a task force of the American Society of Nuclear Cardiology. Journal of Nuclear Cardiology 5(2): 218-231

Elder, S.T.omas; Work, M.S., 1965: Self-determined trains of ICSS delivered to preoptic and forebrain regions as a function of biphasic rectangular pulse frequency. Three rats with electrodes implanted in the preoptic area were trained to administer trains of rectangular biphasic pulses to their own brains for as long as they held a lever down. With pulse frequency as the independent variable it was found tha...

G.X.aoFeng; Tang XianYing; Luo ZhengRong, 2001: Study on the establishment of separation system for unreduced pollens in 'Zenjimaru' persimmon variety. Studies were conducted to investigate the identification, screening and recovery of the viability of unreduced pollen (2n pollen) in Japanese persimmon (Diospyros kaki) cultivar Zenjimaru. The diameter of normal pollen and unreduced pollen was 50...

Cook, M.T., 1936: Phloem necrosis in the stripe disease of Corn. Examination of maize affected with white stripe disease in Porto Rico showed phloem necrosis to be invariably present, accompanied by a thickening of the walls of the epidermal cells, fibrous cells, and sheath. The chloroplasts in the cells of hea...

Kager, L.M.; Bemelman, W.A.; Bastiaansen, B.A., 2016: Chronic recurrent appendicitis: a contradiction in terms?. For two years, every 6 weeks a 23-year-old man presented with episodes of severe abdominal pain and fever. After an extensive diagnostic workup, showing only slightly increased infectious parameters during the pain attacks and infiltration around...

Zhao, H-Ju.; Ma, J-Ping.; Liu, Q-Kui.; Dong, Y-Bin., 2013: Zn(II) and Cu(II) complexes generated from 5-(pyridin-4-yl)-3-[2-(pyridin-4-yl)ethyl]-1,3,4-oxadiazole-2(3H)-thione. A new 1,3,4-oxadiazole-containing bispyridyl ligand, namely 5-(pyridin-4-yl)-3-[2-(pyridin-4-yl)ethyl]-1,3,4-oxadiazole-2(3H)-thione (L), has been used to create the novel complexes tetranitratobis{μ-5-(pyridin-4-yl)-3-[2-(pyridin-4-yl)ethyl]-1,3...

Weise, W.; Pfohl, K., 1967: Significance of the sum total of the width of cuspids, bicuspids and 6-year molars on the variation in the width of the dental arch under conditions of equal width of the incisors in the normal dentition. Zahnarztliche Rundschau 76(3): 84-99

Walsh, J.N.; Taylor, P.A.; Buckley, A.; Darling, T.W.; Schreuer, J.; Carpenter, M.A., 2008: Elastic and anelastic anomalies in Ca,SrTiO3 perovskites; analogue behaviour for silicate perovskites. Values of bulk modulus (K), shear modulus (G) and mechanical quality factor (Q) have been determined for polycrystalline samples across the CaTiO3 (CST0)-SrTiO3 (CST100) solid solution by resonant ultrasound spectroscopy. Because of similarities w...