+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Polyphenols are involved in copper binding to cell walls of Neurospora crassa

, : Polyphenols are involved in copper binding to cell walls of Neurospora crassa. Journal of Inorganic Biochemistry 69(4): 209-215, March

Biochemical and biophysical studies with Neurospora crassa suggested that cellular defense against copper toxicity involves binding of the metal ion to wall associated polyphenols. Growth of N. crassa in presence of toxic amounts of copper (0.63 mM) resulted in the formation of blue coloured mycelia and cell walls wherein most of the copper was wall-associated. Acidic methanol treatment of blue cell walls solubilized apprxeq83% of wall-bound copper along with phenols and phosphate. Phenols isolated from copper-containing blue cell walls were enhanced (2.5 fold) in content when compared to similar extracts made from controls and could precipitate proteins, indicating their polymeric nature. Further, they were more hydrophobic and possessed the intrinsic ability to bind copper reversibly. ESR analysis of copper-polyphenol complex solubilized from blue cell walls showed copper to be paramagnetic, present in a distorted octahedral geometry and bound through oxygens belonging to phenolic hydroxyls and/or nitrite groups. Thin layer chromatography and FTIR spectra of polyphenols isolated from blue cell walls confirmed the presence of nitrophenols. Inhibited nitrate utilization and enhanced tyrosinase activity, occurring under conditions of copper toxicity, seem to contribute to the formation of (poly)nitro-phenols on cell surface through simple physico-chemical mechanisms catalysed by copper.


DOI: 10.1016/s0162-0134(97)10001-0

Other references

Pesenko, YuA., 1975: The fauna and ecology of Apoidea (Hymenoptera) of the Lower Don. 6. A review of the trophic links. Entomological Rev Wash, 543: 53-59

Jarvis, S.P.; Sweetman, A.M.; Lekkas, I.; Champness, N.R.; Kantorovich, L.; Moriarty, P., 2014: Simulated structure and imaging of NTCDI on Si(1 1 1)-7 × 7 : a combined STM, NC-AFM and DFT study. The adsorption of naphthalene tetracarboxylic diimide (NTCDI) on Si(1 1 1)-7 × 7 is investigated through a combination of scanning tunnelling microscopy (STM), noncontact atomic force microscopy (NC-AFM) and density functional theory (DFT)...

Bilanakis, N.; Peritogiannis, V.; Kalampokis, G., 2008: Infections as complications of neuroleptic malignant syndrome. Neuroleptic malignant syndrome is an uncommon but potentially fatal side effect of antipsychotic drug treatment. Several serious complications have been associated with neuroleptic malignant syndrome, such as acute renal failure, deep venous throm...

Lucarelli, F.; Marrazza, G.; Turner, A.P.F.; Mascini, M., 2003: Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors. Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discuss...

Aubry, P.; Loisance, D., 1979: Cardiac surgery and chronic renal failure. Archives des Maladies du Coeur et des Vaisseaux 72(7): 689-690

Scott, C.; Fletcher, R.L.; Bremer, G.B., 1996: Observations on the mechanisms of attachment of some marine fouling blue-green algae. Using scanning electron microscopy (SEM), differential interference contrast microscopy (DICM) and cytochemical staining techniques, preliminary observations have been made on the mechanisms of attachment of some common, marine, benthic fouling bl...

Whittle, H.C.; Aaby, P.; Samb, B.; Jensen, H.; Bennett, J.; Simondon, F., 1999: Effect of subclinical infection on maintaining immunity against measles in vaccinated children in West Africa. Background. Despite a high coverage with measles vaccines in parts of west Africa, epidemics of measles occur with reduced severity in an increasing proportion of older children who have been vaccinated. We examined the effect of exposure to natur...

WeiMing Song;; ZhiXin Li, 2011: Optimization of flue gas turbulent heat transfer with condensation in a tube. Conservation equations for sensible and latent entransy are established for flue gas turbulent heat transfer with condensation in a tube, and the entransy dissipation expression is deduced. The field synergy equation is obtained on the basis of th...

Porfir'eva, O.V.; Yusupova, D.V.; Belyaeva, M.I., 1976: Some aspects of the physiology of serratia marcescens pigmented strains and their nonpigment variants with an elevated nuclease activity. Some aspects of the physiology of S. marcescens pigmentless variants with an elevated nuclease activity were studied. The variants are characterized by a higher respiration rate with glucose, glycerol, inositol or maltose as energy substrates, a h...

Tex A.M.Univ, 1992: Mcdf antiestrogen. Drugs of the Future 17(7): 564-565