geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Preincubation of Dacron grafts with recombinant tissue factor pathway inhibitor decreases their thrombogenicity in vivo


, : Preincubation of Dacron grafts with recombinant tissue factor pathway inhibitor decreases their thrombogenicity in vivo. Journal of Vascular Surgery 24(5): 865-870

Background: We have previously shown that preincubation of whole blood clots with recombinant tissue factor pathway inhibitor (rTFPI) attenuates clot-associated procoagulant activity assessed ex vivo. This study was undertaken to determine whether a single local application of rTFPI induces similar attenuation of the procoagulant activity on preclotted Dacron grafts implanted in an artery in vivo. Methods: Dacron grafts (4 mm times 4 cm long) were preclotted in porcine blood and incubated with either rTFPI (5 mg/ml) or arginine-phosphate buffer for 15 minutes. Grafts were implanted end-to-end in the femoral arteries of 10 pigs, with one rTFPI-treated and one buffer-treated graft implanted in each animal. Animals did not undergo anticoagulation either before or after graft implantation. Radiolabeled porcine fibrinogen was injected intravenously, and the grafts underwent perfusion for 1 hour. A subgroup of animals (n = 7) also had infusion of radiolabeled autologous platelets at the time of administration of radiolabeled fibrinogen. Results: Fibrin(ogen) deposition was decreased in rTFPI-treated grafts by 36% +- 7% (mean +- SEM) compared with buffer-treated grafts (p = 0.001). Platelet deposition was also reduced in the rTFPI-treated grafts by 31% +- 15%, although the reduction did not reach statistical significance (p = 0.10). The extent of rTFPI-mediated attenuation of fibrin(ogen) versus platelet deposition varied independently among animals. Conclusions: Clot-directed anticoagulant effects of rTFPI appear to be useful for substantially decreasing the thrombogenicity of Dacron grafts immediately after their implantation. Chronic studies to determine whether the decreases in thrombogenicity result in improved long-term graft patency appear warranted.

US$19.90

PMID: 8918335

DOI: 10.1016/S0741-5214(96)70024-1


Other references

Ketz Riley, C.J.; Welsh, R.D.; Hoover, J.P., 2003: Fatal septicaemia caused by Streptococcus zooepidemicus in three Atlantic Bottlenose dolphins (Tursiops truncatus). Streptococcus zooepidemicus was found as the cause of the acute death in three Atlantic Bottlenose dolphins (Tursiops truncatus) that had died within three years at a North American zoo. This occurrence has lead to the evaluation of common disease...

Snow, S.; Snow, K., 1992: Sexual dimorphism in mosquito pupae. H.H. Moorefield (1951) [Mosquito News, 11: 175-177] is generally credited with the discovery of sexual dimorphism in mosquito pupae, but it has been known earlier, e.g. in A.E. Shipley (1916) More Minor Horrors. London: Smith, Elder & Co. (with re...

Enomoto, T.; Kitani, T., 1966: Electron microscopic studies on peroxidase and acid phosphatase reaction in human leukocytes (in normal and leukemic cells and on phagocytosis). Nihon Ketsueki Gakkai Zasshi 29(4): 554-570

Hernández Valenzuela, R., 1970: Antimicrobial drugs in pediatrics. Minerva Pediatrica 22(25): 1277-1279

Etzelstorfer, T.; Süess, M.J.; Schiefler, G.L.; Jacques, V.L.R.; Carbone, D.; Chrastina, D.; Isella, G.; Spolenak, R.; Stangl, J.; Sigg, H.; Diaz, A., 2013: Scanning X-ray strain microscopy of inhomogeneously strained Ge micro-bridges. Strained semiconductors are ubiquitous in microelectronics and microelectromechanical systems, where high local stress levels can either be detrimental for their integrity or enhance their performance. Consequently, local probes for elastic strain...

Wang, W.C.; Gallagher, D.M.; Pegelow, C.H.; Wright, E.C.; Vichinsky, E.P.; Abboud, M.R.; Moser, F.G.; Adams, R.J., 2000: Multicenter comparison of magnetic resonance imaging and transcranial Doppler ultrasonography in the evaluation of the central nervous system in children with sickle cell disease. To compare the results of standardized magnetic resonance imaging (MRI) of the brain and transcranial Doppler (TCD) ultrasonography of cerebral arteries in school-aged children with sickle cell disease to determine the correlation between these tw...

Falces, C.; Andrea, R.; Heras, M.; Vehí, C.; Sorribes, M.; Sanchis, L.; Cevallos, J.; Menacho, I.; Porcar, Sílvia.; Font, D.; Sabaté, M.; Brugada, J., 2011: Integration between cardiology and primary care: impact on clinical practice. To assess the impact of a program integrating cardiology and primary care in clinical practice, compared with usual care. The integrated care consists of a hospital cardiologist in each primary care clinic, shared clinical history, joint practice...

C.R.P.vey; C.E.M.N.no, 2009: Bird assemblages of arid Australia Vegetation patterns have a greater effect than disturbance and resource pulses. Arid birds are known to be highly responsive to spatio-temporal changes in food and water availability, and it is thought that this ‘resource tracking’ will obscure predictable patterns of community structure. Here we examine this reso...

Terao, H.; Ikeda, A.; Kobayashi, S.; Teraoka, A., 1978: A thrombosed giant aneurysm of the internal carotid artery with brain stem displacement. A giant aneurysm with a maximum diameter of 5.2 cm arising from the right internal carotid artery grew to occupy the interpenduncular cistern and displaced the upper brain stem. Most of the lumen of the aneurysm was occluded by a thrombus and its...

Krauter, E.M.; Strong, D.S.; Brooks, E.M.; Linden, D.R.; Sharkey, K.A.; Mawe, G.M., 2007: Changes in colonic motility and the electrophysiological properties of myenteric neurons persist following recovery from trinitrobenzene sulfonic acid colitis in the guinea pig. Persistent changes in gastrointestinal motility frequently accompany the resolution of colitis, through mechanisms that remain to be determined. Trinitrobenzene sulfonic acid (TNBS) colitis in the guinea pig decreases the rate of propulsive motili...