+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Preliminary studies on beta-lactamases from aztreonam-resistant Enterobacter cloacae

, : Preliminary studies on beta-lactamases from aztreonam-resistant Enterobacter cloacae. Zhongguo Yaolixue Yu Dulixue Zazhi 8(4): 297-300

The high level beta-lactamases producing from 4 strains of aztreonam (Azt)-resistant Enterobacter cloacae (RAzt; minimal inhibitory concentration gt 50 mg cntdot L-1) were studied in substrate hydrolysis profile, susceptibility to beta-lactamase inhibitors and isoelectric point (pI) by using ultraviolet spectrophotometry and super-thin layer isoelectric focusing electrophoresis. The results showed that the beta-lactamases from RAzt 1029 and 855 hydrolyzed cephalosporins more strongly than penicillins, could be inhibited by cloxacillin (MCIPC) or Azt, but not by CP45899, pI gt 8.0. It suggested that the enzyme probably belonged to chromosome-mediated Richmond and Sykes type I beta-lactamase (e.g. P99). The beta-lactamase from RAzt 430 hydrolyzed penicillins and cephalosporins equally, could be inhibited by either MCIPC or CP45899. but not by Azt, pI is the same as TEM-1 (5.5). it suggested that the enzyme probably belonged to plasmid -mediated Richmond and Sykes type III beta-lactamase (e.g. TEM-I). The beta-lactamase from RAzt 875 hydrolyzed cephalosporins and penicillins equally, could only be inhibited by the combination (instead of their any single use) of CP45899 and MCIPC or CP45899 and Azt, which presented two bands (pI 8.6 and 5.5) on the isoelectric focusing electrophoresis pattern. It suggested that RAzt 875 had two types of beta-lactamases.


Other references

Drouet D'Aubigny, F.; Roquelaure, Y.; Bertrand, L.; Caillon, M.; Calès, P., 1998: Hepatitis imputable to dimethylformamide with reintroduction. Gastroenterologie Clinique et Biologique 22(8-9): 745-746

Anon., 1956: Lotus flowers raised from centuries-old seed. The article, which was made available by the Chinese Embassy in East Germany, describes lotus plants (Nelumbo nucifera) raised from seeds at least 200 years old. The seeds were found buried in the silt of a pond which dried out 190-280 years ago....

de Winter, J.P.; van Sonderen, L.; Egberts, J.; van Bel, F., 1992: Surfactant therapy in respiratory distress syndrome; a review. Nederlands Tijdschrift Voor Geneeskunde 136(41): 2010-2013

Milbradt H.; Cancho E.C.; Qaiyumi S.A.A.; Galanski M., 1990: Ultrasound examination of the wrist and hand. Sonographic examination of the hand requires high-frequency linear transducers. As the relevant structures are located very close to the surface, water stand-off pads are mandatory. Owing to the high sensitivity of sonography in the detection of f...

Fischer-Tenhagen, C.; Tenhagen, B-A.; Heuwieser, W., 2013: Short communication: Ability of dogs to detect cows in estrus from sniffing saliva samples. Efficient estrus detection in high-producing dairy cows is a permanent challenge for successful reproductive performance. In former studies, dogs have been trained to identify estrus-specific odor in vaginal fluid, milk, urine, and blood samples u...

Király, J.; Czeizel, E., 1986: Evaluation of occupational teratogenic hazards based on data from the Hungarian Case-Control Surveillance System for Congenital Anomalies. Orvosi Hetilap 127(31): 1871-1876

Cheung, H.Y.S.; Chung, C.C.; Fung, J.T.K.; Wong, J.C.H.; Yau, K.K.K.; Li, M.K.W., 2007: Laparoscopic resection for colorectal cancer in octogenarians: results in a decade. This study was designed to evaluate the results of laparoscopic resection for colorectal cancer in octogenarians. Patients aged 80 years or older who underwent elective laparoscopic resection for colorectal cancer from July 1, 1996 to June 30, 200...

Jha, A.Kumar., 2016: Secretary's Message. Indian Journal of Nuclear Medicine 31(Suppl 1): S2-S2

Hartill W.F.T., 1982: Control of downy mildews of brassica seedlings and lettuce with systemic fungicides. Metalaxyl, used in granules presowing, in drenches at seedling emergence or in routine spray applications during plant growth, gave good control of downy mildew in brassica seedbeds (caused by Peronospora parasitica (Pers. ex Fr.) Fr.) and of down...

Ravosa, M.J., 1991: Ontogenetic perspective on mechanical and nonmechanical models of primate circumorbital morphology. Dimensions of the supraorbital torus, postorbital bar, and postorbital septum were collected in an ontogenetic series of Macaca fascicularis and compared with expectations based on models that attribute morphological variation in these features to...