+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Proliferative responses and gamma interferon and tumor necrosis factor production by lymphocytes isolated from tracheobroncheal lymph nodes and spleens of mice aerosol infected with Bordetella pertussis

, : Proliferative responses and gamma interferon and tumor necrosis factor production by lymphocytes isolated from tracheobroncheal lymph nodes and spleens of mice aerosol infected with Bordetella pertussis. Infection & Immunity 60(11): 4563-4570

A group of mice was aerosol infected with live, virulent Bordetella pertussis bacteria. During a period of 7 weeks following the infection, with intervals of 1 week, lymphocytes were isolated from the tracheobroncheal lymph nodes (TBL) and the spleens (SPL) of the infected mice. The in vitro proliferative responses as well as the gamma interferon and tumor necrosis factor production levels of the isolated lymphocytes in response to stimulation with whole killed B. pertussis bacteria were measured as parameters for cell-mediated immunity (CMI). The course of the infection was monitored by counting of CFU in the lungs of the mice. Moreover, antibody responses in serum against a range of B. pertussis antigens were assessed. The results that vigorous proliferative response of the TBL and SPL to stimulation with whole killed B. pertussis bacteria was induced by the infection. The proliferative response of the TBL was significantly higher than the response of the SPL. The proliferative responses were maximal 3 to 4 weeks after the infection and were paralleled by in vitro gamma interferon and tumor necrosis factor production upon specific stimulation. The development of the CMI was observed simultaneously with clearance of the infection from the lungs. Antibody responses became measurable in the sera only after the infection was cleared. A specific CMI against pertussis toxin, the filamentous hemagglutinin, the 69-kDa outer membrane protein, and the agglutinogens and 2 3, antigens which are under consideration for inclusion in future acellular pertussis vaccines, was successfully demonstrated in mice 3 weeks after the infection.


PMID: 1398968

Other references

Tousignant, J.; Côté, B.; Barolet, D., 1993: Urticaria and antihistamines. Urticaria is often incapacitating, yet it is not always easy to know whether a patient should undergo testing and what treatment should be prescribed. This article provides a systematic approach to urticaria and its treatment.

Mehta, N.; Butala, P.; Bernstein, M.P., 2012: The imaging of maxillofacial trauma and its pertinence to surgical intervention. Maxillofacial skeletal injuries account for a large proportion of emergency department visits and often result in surgical consultation. Although many of the principles of detection and repair are basic, the evolution of technology and therapeutic...

Krempler, F., 1984: Metabolism and possible mechanisms of atherogenesis induced by lipoprotein (a). The mechanisms of atherogenesis are under intensive clinical and experimental investigation. It is commonly accepted that lipoproteins play a major role in atherogenesis. The results of several clinical studies suggest that lipoprotein (a) [Lp(a)]...

Davies, B.M.; Jones, A.; Patel, H.C., 2015: Surgical-site infection surveillance in cranial neurosurgery. Surgical-site infection (SSI) is associated with significant morbidity and mortality. Public Health England or PHE has published guidance on its surveillance, which is now mandatory in some specialities. We review how appropriate their programme i...

Vo, T.T.; Parrish, D.A.; Shreeve, J'ne.M., 2014: Tetranitroacetimidic acid: a high oxygen oxidizer and potential replacement for ammonium perchlorate. Considerable work has been focused on developing replacements for ammonium perchlorate (AP), a primary choice for solid rocket and missile propellants, due to environmental concerns resulting from the release of perchlorate into groundwater system...

Luo, Z., 1981: A systematic ewrevi of the Chinese cape hare, Lepus capensis Linnaeus. Acta Theriologica Sinica, 12: 149-157

Richardson, D.E.; Cowen, R.K., 2004: Diversity of leptocephalus larvae around the island of Barbados (West Indies): relevance to regional distributions. The cryptic nature of many elopomorph species and their occupation of minimally explored habitats have led to the underestimation of their importance as a component of regional biodiversity. Additionally, it had led to their exclusion from studies...

Kosic, I.V.; Anicic, B., 2005: Investigation of sociological aspects of the Park Maksimir. This study was conducted to examine the sociology of tourism in Maksimir Park (Croatia) by analysing the existing literature as well as the data obtained through interviews with park owners, faculty of the Forestry Department, and faculty of Agric...

Gunton, R.-M.K.nin, W., E., 2007: Density effects at multiple scales in an experimental plant population. 1 In sessile organisms, each individual in a population experiences a different local conspecific density, and this density itself will vary depending on the scale at which it is measured (converging on a 'mean field' population density...

Lackey R.T., 1975: Environmental assessment a comparison of approaches and methods. Virginia Journal Of Science: 58