geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Prostaglandin E2 enhances human endometrial stromal cell differentiation


, : Prostaglandin E2 enhances human endometrial stromal cell differentiation. Endocrinology 134(1): 258-263

Endometrial stromal differentiation (decidualization) is essential for implantation of the developing blastocyst. Because prostaglandins (PGs) are synthesized in the endometrium, and PG-binding sites have been demonstrated in the proliferative endometrial stromal cell (the precursor of the decidual cell), experiments were performed to determine whether PGs are involved in the process of decidualization. Human endometrial stromal cells were cultured for 18 days in Dulbecco's Modified Eagle's Medium-2% fetal bovine serum with 1 mu-M medroxyprogesterone acetate (MPA) and 10 nM estradiol, with and without PGE-2 or PGF-2alpha. Expression of PRL was used as a marker of decidualization. In the presence of estradiol and MPA alone (control), PRL was detected beginning on day 9 and gradually increased through day 18. In contrast, PRL was detected on day 3 in the PGE-2-treated cells, and the magnitude of stimulation in these cells on days 9-12 was 13001400% of that in control cells. Furthermore, the PRL mRNA content of the PGE-2-treated cells on day 12 was 4.6-fold greater than that in the control cells. The effect of PGE-2 on PRL production was dose dependent, with a minimal effective dose of 10-10 M. PGE-2 in the absence of steroids had a minimal effect on PRL production. In contrast to PGE-2, PGF-2alpha treatment had no effect on PRL expression in steroid-treated cells. These results indicate that there are synergistic effects among PGE-2, estradiol, and MPA, resulting in acceleration of endometrial stromal cell differentiation and enhanced PRL expression.

US$19.90

PMID: 7506205

DOI: 10.1210/endo.134.1.7506205


Other references

Webster, A.J.F.; Smith, J.S.; Crabtree, R.M.; Mollison, G.S., 1976: Prediction of the energy requirements for growth in beef cattle. 2. Hereford X British Friesian steers given dried grass or barley. For part 1 see NAR 46, 699.2. Hereford X British Friesian castrate male cattle, growing from about 80 to 500 kg, were given equal amounts of metabolizable energy (ME) from dried grass pellets or from a pelleted diet of barley and a protein supplem...

Visek, W.J.; Jacobson, A.I.; Iwert, M.E.; Alvarez, A.P., 1961: Depression of urease activity in the gastro-intestinal tract by chemical agents. FED PROC 20(1 Pt 1): 370

McGovern, D.A.; Doorley, G.W.; Whelan, A.M.; Parker, A.W.; Towrie, M.; Kelly, J.M.; Quinn, S.J., 2009: A study of the pH dependence of electronically excited guanosine compounds by picosecond time-resolved infrared spectroscopy. The photophysical properties of 5'-guanosine monophosphate (5'-GMP) and polyguanylic acid {poly(G)} in D(2)O solutions of varying pH have been studied using picosecond transient infrared absorption spectroscopy. Whereas in neutral or wea...

Demir, R.; Zeren, M.; Gurses, H.Nilgun.; Yigit, Z., 2017: Relationship of respiratory muscle strength, pulmonary function, and functional capacity with quality of life in patients with atrial fibrillation. Objective To examine the relationship of pulmonary parameters and functional capacity with quality of life (QoL) in patients with atrial fibrillation (AF). Methods Thirty-six patients with chronic AF were included in this cross-sectional study. Qo...

Kaverina, E.N.; Akopian, T.A.; Denisova, T.S.; Karpov, V.A.; Ponomareva, T.I.; Naroditskii, B.S.; Tikhonenko, T.I., 1993: Polymerase chain reaction analysis of Rat2 cells transformed by the CELO (FAV1) virus. DNAs extracted from the RAT2 cells transformed by the CELO virus (strain Phelps) have been analyzed by polymerase chain reaction. Sequences belonging to the left end of the viral genome (according to Ishibashi and Yasue, 1984) were not found in th...

Rosso, C.; Drier, A.; Lacroix, D.; Mutlu, G.; Pires, C.; Lehéricy, S.; Samson, Y.; Dormont, D., 2010: Diffusion-weighted MRI in acute stroke within the first 6 hours: 1.5 or 3.0 Tesla?. To compare the sensitivity and specificity of 1.5-T and 3.0-T diffusion-weighted MRI (DWI) to detect hyperacute ischemic stroke lesions. We blindly reviewed the DWI of 135 acute stroke patients and 34 controls performed at 1.5 T (n = 108) or 3.0 T...

Winchell Newton Horace, 1906: Deep wells as a source of water for Minneapolis. Bulletin - Minnesota Academy of Natural Sciences 4: 266

Mengoni, A.; Barzanti, R.; Gonnelli, C.; Gabbrielli, R.; Bazzicalupo, M., 2001: Characterization of nickel-resistant bacteria isolated from serpentine soil. In the present study, heterotrophic nickel-resistant bacteria were isolated and characterized from three different serpentine outcrops in central Italy, populated by the nickel-hyperaccumulating plant Alyssum bertolonii. Bacteria were isolated fro...

Pinkas, L.L.H., 1967: An analysis of the early- and mid-season growth of Pima cotton varieties S-3 and S-4 at two altitudes. The results showed that differences between the varieties at the two altitudes were determined by internode length. Yield differences are attributed to varietal differences in fertilization, boll set and boll abscission.

Roepke, J.E.; Heifetz, S.A., 1997: Pathological case of the month. Epithelioid hemangioendothelioma (intravascular bronchioloalveolar tumor) of the lung. Archives of Pediatrics & Adolescent Medicine 151(3): 317-319