+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Purification and characterisation of D-glucose oxidase from white-rot fungus Pleurotus ostreatus

, : Purification and characterisation of D-glucose oxidase from white-rot fungus Pleurotus ostreatus. European Journal of Biochemistry 215(3): 747-752

D-Glucose oxidase was purified 27.5-fold to apparent homogeneity with an overall yield of 23.8%, from Pleurotus ostreatus, through a purification procedure of ammonium sulphate precipitation, gel-permeation, anion-exchange and hydrophobic-interaction chromatography. The molecular mass determined by gel filtration was found to be 290 kDa. SDS/PAGE revealed that the enzyme consists of four subunits with a molecular mass of 70 kDa. The absorption spectra of the enzyme exhibit maxima at 280, 360 and 460 nm. The enzyme shows a fluorescence spectrum with an excitation maximum at 470 nm and an emission maximum at 530 nm. These results indicate that the prosthetic group of the enzyme is flavin and that the enzyme contains 4 mol flavin/mol enzyme. The enzyme is optimally active at 50 degrees C and at pH 5.5-6.0. It exhibits broad affinity for various sugars and specificity for D-glucose with Km value of 1.34 mM. 2,6-Dichloroindophenol, Wurster's blue, and 4-benzoquinone can function as electron acceptors but phenazine methosulphate cannot function as an electron acceptor. The enzyme is inhibited completely by mercuric chloride and partially by silver sulphate, sodium azide and 8-hydroxyquinoline.


PMID: 8354282

DOI: 10.1111/j.1432-1033.1993.tb18088.x

Other references

Pombo, Míra.; Turra, A., 2015: Issues to be considered in counting burrows as a measure of Atlantic ghost crab populations, an important bioindicator of sandy beaches. The use of indirect estimates of ghost-crab populations to assess beach disturbance has several advantages, including non-destructiveness, ease and low cost, although this strategy may add some degree of noise to estimates of population parameters...

Wei Zhili; Zhou Liqing, 1996: The regularity of oil and gas abundant accumulation in North Jiangsu Basin, China. International Geological Congress, Abstracts = Congres Geologique International, Resumes 30, Vol

Chapron, C., 1971: Relations entre les evenements morphogenetiquesde la regeneration et les syntheses d'acides ribonucleiques et de proteines. Etude chez Eisenia foetida (Oligochaeta). Comptes r hebd Seanc Acad Sci Paris, 272D 859-862

Kinne, O.; Rosenthal, H., 1967: Effects of sulfuric water pollutants on fertilization, embryonic development and larvae of the herring, Chipea harengus. Using herring (Clupea harengus) eggs and larvae from "Baltic spring spawners", the biological effects of sulfuric pollutants (largely FeSO4 and H2SO4) which are scheduled to be released daily into the North Sea in large quantities, have...

Necek S.; Bergmann H., 1990: Concept of the emergency admission unit in a major community hospital. Der Anaesthesist 39(10): 463-468

Bassett, P., 1985: Tarsonemid mites. Biological pest control: the glasshouse experience edited by NW Hussey and N Scopes: 6

Szego, C.M.; Lippe, B.M., 1965: Mediation by adrenocortical hypersecretion of the suppressive influence of actinomycin D on uterine estrogen sensitivity. Steroids: Suppl 2:235-47

Mahieu, P.R.; Foidart, J.B.; Dubois, C.H.; Dechenne, C.A.; Deheneffe, J., 1980: Tissue culture of normal rat glomeruli: contractile activity of the cultured mesangial cells. Rat glomerular epithelial and mesangial cells, and rat skin fibroblasts, were cultured in RPMI medium containing 15% decomplemented fetal bovine serum. They were incubated for 1 h at C with 2 mM [Ca2+]. At that time, their response to v...

Bayston, R.; Ashraf, W., 2016: Treatment of prosthetic joint infections due to Propionibacterium. Acta Orthopaedica 87(1): 87-87

Kurokawa, Y.; Komiyama, A., 1998: Döhle body. Ryoikibetsu Shokogun Shirizu: 96-97