+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Quercetin protects cutaneous tissue-associated cell types including sensory neurons from oxidative stress induced by glutathione depletion: cooperative effects of ascorbic acid

, : Quercetin protects cutaneous tissue-associated cell types including sensory neurons from oxidative stress induced by glutathione depletion: cooperative effects of ascorbic acid. Free Radical Biology & Medicine 22(4): 669-678

Oxidation reactions are essential biological reactions necessary for the formation of high-energy compounds used to fuel metabolic processes, but can be injurious to cells when produced in excess. Cutaneous tissue is especially susceptible to damage mediated by reactive oxygen species and low-density lipoprotein oxidation, triggered by dysmetabolic diseases, inflammation, environmental factors, or aging. Here we have examined the ability of the flavonoid quercetin to protect cutaneous tissue-associated cell types from injury induced by oxidative stress, and possible cooperative effects of ascorbic acid. Human skin fibroblasts, keratinocytes, and endothelial cells were cultured in the presence of buthionine sulfoximine (BSO), an irreversible inhibitor of glutathione (GSH) synthesis. Depletion of intracellular levels of GSH leads to an accumulation of cellular peroxides and eventual cell death. Quercetin concentration-dependently (EC-50: 30-40 mu-M) reduced oxidative injury of BSO to all cell types, and was also effective when first added after BSO washout. BSO caused marked decreases in the intracellular level of GSH, which remained depressed in quercetin-protected cells. Ascorbic acid, while by itself not cytoprotective synergized with quercetin, lowered the quercetin EC-50 and prolonged the window for cytoprotection. The related flavonoids rutin and dihydroquercetin also decreased BSO-induced injury to dermal fibroblasts, albeit less efficaciously so than quercetin. The cytoprotective effect of rutin, but not that of dihydroquercetin, was enhanced in the presence of ascorbic acid. Further, quercetin rescued sensory ganglion neurons from death provoked by GSH depletion. Direct oxidative injury to this last cell type has not been previously demonstrated. The results show that flavonoids are broadly protective for cutaneous tissue-type cell populations subjected to a chronic intracellular form of oxidative stress. Quercetin in particular, paired with ascorbic acid, may be of therapeutic benefit in protecting neurovasculature structures in skin from oxidative damage.


PMID: 9013129

DOI: 10.1016/s0891-5849(96)00383-8

Other references

Noguer, T.; Marty, J.L., 1997: Reagentless sensors for acetaldehyde. A reagentless acetaldehyde sensor is designed based on the co-entrapment of aldehyde dehydrogenase (AlDH), NADH oxidase and high molecular weight NADs (NAD-dextran and NAD-PEG) in a polyvinyl alcohol) bearing stryrylpyridinium groups (PVA-SbQ) mat...

Leibundgut, Hans, 1954: Forests and silviculture in Switzerland. This paper includes a brief description of the distribution and composition of the natural forests of Switzerland, their economic significance, and their past treatment. Diversity of conditions had led to corresponding diversity in silvicultural t...

Gatti, R.; Cappellin, E.; Zecchin, B.; Antonelli, G.; Spinella, P.; Mantero, F.; D.P.lo, E.Franco., 2005: Urinary high performance reverse phase chromatography cortisol and cortisone analyses before and at the end of a race in elite cyclists. A functional and basic method for the quantitative analysis of urine cortisol (F) and cortisone (E) using a Solid-Phase Extraction column and HPLC with ultraviolet detection is here described and validated to analyse urine samples. Urine specimens...

Häuser, W.; Stallmach, A.; Caspary, W.F.; Stein, J., 2007: Predictors of reduced health-related quality of life in adults with coeliac disease. Background Data available on predictors of reduced health-related quality of life in coeliac disease are not consistent. Aim To test predictors of reduced health-related quality of life, described in the literature, by a multivariate approach.Met...

Gronroos, E.; Schippert, A.; Andersson, T.; Sjolander, A., 1993: Effects of an impaired isoprenoid metabolism on LTD-4-induced Ca-2+ signaling in human epithelial cells. Molecular Biology of the Cell 4(SUPPL ): 8A

Klein, J.R.ymond; Kamin, H., 1941: Inhibition of the d-amino acid oxidase by benzoic acid. The rate of oxida-tion of d-amino acids by rat liver and kidney slices and by various preps. of the d-amino acid oxidase was inhibited by benzoic acid. The data obtained suggested that the inhibitory effect was due to the formation of a benzoic ac...

Val'kyunas, G.A., 1987: Blood parasites of birds with white sea baltic migrational direction 4. ecological aspect. The effect of some ecological factors on the infection of birds with blood Protozoa was studied. Most favourable conditions for circulation of Haemoproteidae on the Kurish Spit were recorded in July. The possibility of the infection of young small...

Guggemos, A.; Juen, F.; Engelmann, L.; Diesselhorst, V.; Henze, Günter.; D.G.llo, A., 2015: Siblings of children with cancer--the price they pay to function. Supportive Care in Cancer 23(7): 1837-1839

Christophersen E.B., 1968: A inst pulsatile intra aortic balloon for ventricular assist experimental inst arteriographic observations calf inst angiography inst electro cardiography. Radiology: 493-496

Lee, J.Yun.; Kim, E.Young.; Cho, E., 2012: Factors impacting the physical function of older adults in Korean long-term care hospitals. This study was conducted to examine activities of daily living (ADL) of older adults admitted to Korean long-term care hospitals (LTCHs), and to explore the patient and organizational factors that have an impact on the ADL of this population. A se...