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Rapid assay of HSF1 and HSF2 gene expression by RT-PCR

, : Rapid assay of HSF1 and HSF2 gene expression by RT-PCR. Molecular & Cellular Biochemistry 158(2): 189-192

Primers were developed to allow the rapid and reliable assay of heat shock transcriptional factors 1 and 2 in human epidermoid A431 cells by following the protocol described in this study. Using the primers, the heat-induced increase in heat shock transcriptional factor 1 but not 2 was observed. This is the first report to show that heat shock increases the mRNA amount of HSF1 with no changes in HSF2 mRNA.


PMID: 8817481

DOI: 10.1007/bf00225845

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