+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Regulation mechanisms of intracellular pH of Xenopus laevis oocyte

, : Regulation mechanisms of intracellular pH of Xenopus laevis oocyte. Biochimica et Biophysica Acta 1137(1): 45-51

Intracellular pH values (pHi) of Xenopus oocytes were optically measured using a fluorescent dye, 2', 7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF). The oocytes were loaded with dye by incubation with a membrane-permeable form (BCECF-AM). Mean pHi of the oocytes in pH 7.6 solution was 7.69. Increasing ambient pCO2 rapidly decreased pHi and estimated buffering power was 23.8 mM/pH unit. Changing ambient HCO3- from 5 to 30 mM did not alter pHi. After incubation in a Na(+)-free solution, Na+ addition to the bath rapidly increased pHi and this response was blocked by amiloride (ED50 2 microM). The addition of NH4Cl to the bath caused an initial transient increase in PHi followed by a secondary decrease. The secondary decrease was greatly inhibited by a histidine specific reagent, diethylpyrocarbonate. It was also slightly inhibited by ouabain, Ba2+ and furosemide, but not by amiloride. These data suggest that (1), fluorescence technique is applicable to PHi measurements of Xenopus oocytes; (2), Xenopus oocytes have an amiloride sensitive Na+/H(+)-exchange, and permeabilities to CO2, NH3, and NH+4. These observation may be useful in studying the relationship between pHi and oocytes development, and the expression of acid/base transporters in Xenopus oocytes.


PMID: 1327152

DOI: 10.1016/0167-4889(92)90098-v

Other references

Danenberg, A.H., 1997: 31 financial check-off points. Dentistry Today 16(7): 92-95

Cordero, P.; Juan, R.; Gonzalez, E., 1985: Response of dark tobacco cv. Criollo to different levels of soil moisture. In field trials in Jan.-March 1979-82, dark tobacco cv. Criollo was either unirrigated or subjected to 3 different sprinkler irrigation regimes, maintaining soil moisture at 80% field capacity from transplanting to the 10-leaf stage, 65-80% from t...

Doust, J.A.; Pietrzak, E.; Sanders, S.; Glasziou, P.P., 2005: Identifying studies for systematic reviews of diagnostic tests was difficult due to the poor sensitivity and precision of methodologic filters and the lack of information in the abstract. Methods to identify studies for systematic reviews of diagnostic accuracy are less well developed than for reviews of intervention studies. This study assessed (1) the sensitivity and precision of five published search strategies and (2) the relia...

Mruk Luczkiewicz, A., 1981: Analysis of lipids occurring in seeds of the genus Salvia. Oil content, determined in seeds of 15 Salvia spp., ranged from 31 to 38% in S. regeliana, S. przewalskii, S. sclarea and S. hispanica, and from 23 to 28% in the other species. Data are tabulated on the composition of fatty acids. The highest cont...

Rodriguez-Roisin, R.; Barberà, J.A., 1993: Chronic obstructive pulmonary disease: mechanism of hypoxaemia (structure and gas exchange relationships). Monaldi Archives for Chest Disease 48(5): 415-417

Penttinen R.; Kulonen E., 1970: Carbohydrates in the healing fracture callus of rat. Scandinavian Journal of Clinical & Laboratory Investigation Supplement 25(113): 32

Vermeijden, J.Wytze.; Zijlstra, J.G.; Erasmus, M.E.; van der Bij, W.; Verschuuren, E.A., 2009: Lung transplantation for ventilator-dependent respiratory failure. Lung transplantation of patients on mechanical ventilation is controversial, but successful transplantation of these patients has been reported. This report describes our institutional experience with lung transplantation of mechanically ventilate...

Prado, A., 1942: Um nova casa de bicefalia em Serpente. Ciencia Mexico, 38-9: 254-255

Anon; Iups Iscerg Symposium Brighton; 1191 1223, 1971: Iups iscerg symposium brighton 1971. Vision Research 11(10): 1191-1223

Suchecki, K.; Hołyst, J.A., 2006: Ising model on two connected Barabasi-Albert networks. We investigate analytically the behavior of the Ising model on two connected Barabasi-Albert networks. Depending on relative ordering of both networks there are two possible phases corresponding to parallel or antiparallel alignment of spins in bo...