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Regulation of the vasomotor activity of lymph microvessels by nitric oxide and prostaglandins


, : Regulation of the vasomotor activity of lymph microvessels by nitric oxide and prostaglandins. American Journal of Physiology 274(3 Pt 2): R790-R796

It was shown previously that the presence of endothelium modulates spontaneous vasomotion of small lymphatic vessels. In the present study, we aimed to elucidate the nature of endothelium-derived factors, produced in basal conditions and in response to agonists, that affect the smooth muscle tone of lymph microvessels in vitro. Afferent lymph microvessels were isolated from rat iliac lymph nodes, cannulated with glass micropipettes, and pressurized (6 cmH2O), and changes in their diameter were investigated with video microscopy. In resting conditions, isolated lymph vessels exhibited spontaneous constrictions and dilations. The maximum and minimum diameters (Dmax and Dmin) were 149.8 +/- 2.9 and 85.8 +/- 3.6 microns, respectively. Acetylcholine (ACh, 10(-7) to 10(-5) M) and sodium nitroprusside (SNP, 10(-8) to 10(-6) M) temporarily abolished diameter oscillations, increasing the diameter of lymphatics dose dependently. For example, 10(-5) M ACh and 10(-6) M SNP increased the diameter (Dmax) by 15.2 +/- 2.2 and 25.0 +/- 2.7 microns, respectively. Treatment of vessels with NG-nitro-L-arginine (10(-4) M) significantly reduced the amplitude of diameter oscillations and nearly completely eliminated ACh-induced dilation of lymph microvessels, whereas SNP (10(-6) M) elicited a significantly greater dilation (55.6 +/- 7.5 microns). Arachidonic acid (AA, 10(-8) to 10(-6) M) constricted (up to 50 microns), whereas prostaglandin E2 (PGE2, 10(-9) to 10(-7) M) dilated (up to 40 microns), lymphatic vessels. Indomethacin (10(-5) M) increased both Dmax and Dmin and completely inhibited AA-induced constrictions, but did not affect PGE2-induced dilations of lymph microvessels. AA-induced constrictions of lymphatics were converted into dilations after treatment with SQ-29,548, a selective PGH2-thromboxane A2 (PGH2-TxA2, 10(-6) M) receptor antagonist, whereas PGE2-induced dilations were not affected. We conclude that endothelial nitric oxide and prostaglandins are important modulators of lymphatic vasomotion, hence pumping activity of lymph microvessels in vivo.

US$29.90

PMID: 9530247


Other references

Kwiterovich P.O.; Levy R.I.; Fredrickson D.S., 1970: Early detection and treatment of familial type ii hyper lipo proteinemia. Circulation Research Supplement 42(4-3): III-11

Zavaritsky V.A., 1938: On the question of the Berikul phase of tectogenesis in the Altai region. The Berikul phase of tectogenesis in the Kuznetsk Alatau, Altai region, western Siberia, was established on the basis of a younger granodiorite intrusion traversing an older syenite intrusion. However, this phase is not valid in that petrological...

LoPachin, R.M.; Moore, R.W.; Menahan, L.A.; Peterson, R.E., 1984: Glucose-dependent lactate production by homogenates of neuronal tissues prepared from rats treated with 2,4-dithiobiuret, acrylamide, p-bromophenylacetylurea and 2,5-hexanedione. Chronic treatment of rats with dithiobiuret (DTB) produces a delayed onset muscle weakness and, as suggested by a preliminary study, a distal axonopathy. An inhibition of glycolysis resulting in an energy deficit has been suggested as a possible m...

Zenz, M., 1988: Epidural opiates and nerve blocks. Recent Results in Cancer Research. Fortschritte der Krebsforschung. Progres Dans les Recherches sur le Cancer 108: 18-27

Dziecioł, J.; Musiatowicz, B.; Sulkowska, M.; Augustynowicz, A.; Sulkowski, S., 1996: Usefulness of nucleolar organiser region staining (AgNORs) in the evaluation of follicular tumours of the thyroid. Folia Histochemica et Cytobiologica 34 Suppl 1: 35-36

de Bruin, T.R., 1966: Anastomosis of the intestinal tract in the infant. International Surgery 45(1): 105-107

Bagagem, M.da.P., 1992: The role of the nurse specialist in mental health and psychiatric nursing on the health team in the view of new concepts. Servir 40(4): 182-186

Staley Richard M., 1988: Use of Appleworks for a New England earthquake data base to teach junior high school students about New England data. Abstracts with Programs Geological Society of America: , Pages 68. 1988.

Tolle, F.; Rosenthal, M.; Pfeiffer, F.; Mayer, Günter., 2016: Click Reaction on Solid Phase Enables High Fidelity Synthesis of Nucleobase-Modified DNA. The post-synthetic functionalization of nucleic acids via click chemistry (CuAAC) has seen tremendous implementation, extending the applicability of nucleobase-modified nucleic acids in fields like fluorescent labeling, nanotechnology, and in vitr...

Budzier, H.H., 1967: Microbiological study of ensiling feedstuffs. 1. Effects of metabisulphite and kofa salt on changes in bacteria. Metabisulphites, 0.25% to 0.5%, added to silage in plastic bags had insufficient effect on Gram-negative bacteria and proteolytic bacteria. Lactic acid bacteria were largely destroyed, mainly in the initial phase of silage making. Kofa salt, 0.3%,...