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Relationship between functional properties and structure of ovalbumin


, : Relationship between functional properties and structure of ovalbumin. Journal of Protein Chemistry 13(2): 261-274

The effects of ovalbumin (OVA) denaturation using urea, guanidinium chloride (GdnHCl), sodium dodecyl sulphate (SDS), cetylpyridinium chloride (CPC), 3-((3-cholamidopropyl)dimethylammonio)-1-propanesulfonate (CHAPS), and 5 different cationic detergents with various side chains, HCl, and CH-3COOH were observed. Progressive unfolding in ovalbumin was measured as a function of fluorescent light intensity, peak response and shift in the maximum of emission. Kinetic measurements demonstrated that the rate of denaturation usually followed a double exponential decay pattern, but at small concentrations of urea and acids first-order reaction was indicated. The reversibility of the unfolding-folding transitions was confirmed from tryptophan fluorescence and circular dichroism (CD) measurements. Differences in secondary structure were observed and changes of alpha-helical content were calculated. Polyacrylamide gel electrophoresis (PAGE) with and without sodium dodecyl sulphate (SDS-PAGE) showed differences in the structure of native and denatured ovalbumin. Native protein samples in PAGE demonstrated smaller number and larger mobilities of subunits than denatured ones with different reductants, such as SDS and 2-mercaptoethanol (2 ME). Scanning of SDS protein patterns showed the appearance of aggregated forms in region of 45 kD.

US$19.90

PMID: 8060498

DOI: 10.1007/bf01891984


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Pallister J.W., 1952: Some glacial features in the neighbourhood of Ecclefechan, Dumfriesshire. Distinguishes five stages of retreat during the final stage of glaciation in the Ecclefechan region, Dumfriesshire, Scotland, on the basis of glacial deposits and drainage channels.

Gallardo, F.; Boethel, DJ., 1990: Effects of the allelochemical, -tomatine, on the soybean looper (Lepidoptera: Noctuidae). To determine the impact of the allelochemical, .alpha.-tomatine, on the soybean looper, Pseudoplusia includens (Walker), various concentrations (0.001 to 0.10% wet weight of diet wwt ) were added to an artificial diet. Neonate larvae were fed on t...

Madonia, T.; Lentini, G.; Raso, D., 1968: Changes of the frequency discrimination threshold in presence of a continuous controlateral sound. La Clinica Otorinolaringoiatrica 20(4): 257-263

Wolffheim, N., 1962: The life course of a neurotic character. I. Praxis der Kinderpsychologie und Kinderpsychiatrie 11: 10-3 Contd

Paterson, I.C.; Willey, R.F.; Shotter, M.V.; Crompton, G.K., 1977: Further studies of rimiterol and salbutamol administered by intermittent positive-pressure ventilation, and an important observation on the technique of using the Bennett ventilator. 1. Using the same technique of administering drugs by intermittent positive-pressure ventilation as used in previous studies a source of contamination of solutions nebulized was discovered. This was rectified by using a new ventilator and complete...

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Nikol'skaya, I.S., 1958: The changes in volume of blood, hemoglobin, and myoglobin during the individual development of astrakhan sheep. Institut Morfologii Zhivot Nykh Akademii Nauk Sssr Moskva

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