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Relationship between platelet density and platelet aggregation, ATP release, and cytosolic-free calcium mobilization in rabbits

, : Relationship between platelet density and platelet aggregation, ATP release, and cytosolic-free calcium mobilization in rabbits. Zhongguo Yao Li Xue Bao 16(4): 367-372

AIM: To study the relationship of platelet density and platelet functions, including aggregation, ATP release, and intracellular calcium mobilization. METHODS: Platelet density was evaluated by discontinuous gradients of Percoll and cytosolic-free calcium concentration in single platelets was measured by fluorescence and imaging. RESULTS: Rabbit platelets were assorted into 3 subpopulations using discontinuous gradients of Percoll: high density (HD, gt 1.062, 20%-30%), intermediate density (ID, around 1.057, 40%-50%) and, low density (LD, lt 1.051, 10%-15%). The sizes of platelets in these subpopulations were widely different (HD: 725 +- 76; ID: 443 +- 47; and LD: 307 +- 46) and well correlated with the density (r = 0.988, P lt 0.01). Thrombin 0.5 U-induced aggregations in the 3 subpopulations were 78 +- 8% 69 +- 4%, and 62 +- 3%, respectively and a difference was found between HD and LD (P lt 0.05). The amounts of ATP released during aggregation were 4.7 +- 0.9, 3.4 +- 1.0, and 2.6 +- 0.6 mu-mol/4 times 10-5 platelets in HD, ID, and LD groups, respectively. Thrombin 150 U-induced (Ca-2+)-i mobilization in HD (990 +- 130 nmol L-1) was different (P lt 0.01) from those in ID (410 +- 80 nmol L-1) and LD (400 +- 40 nmol L-1). Serotonin (5HT) 3 mu-mol L-1 also caused (Ca-2+)-i mobilization in single-washed platelet subpopulations and results were similar to those of thrombin although it did not induce any detectable aggregation or release reaction. CONCLUSION: Platelet functions and intracellular calcium concentration were increased with platelet density in rabbits.


PMID: 7668113

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