geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Role of stress fibers in the association of intermediate filaments with microtubules in fibroblast cells


, : Role of stress fibers in the association of intermediate filaments with microtubules in fibroblast cells. Cell Biology International 17(7): 645-652

The association between intermediate filaments (IF) and microtubules (MT) has been demonstrated by several experiments using MT inhibitors and by microinjecting specific antibodies. The actin cytoskeleton has recently been assigned a role in this process of drug induced IF collapse. However, this was not found to be true in large cells with irregular morphology. For instance, in early passage diploid fibroblasts of human origin and in armadillo cell lines, where the cells are large, irregular in shape and exhibit prominent stress fibers (SF), depolymerization of MT with nocodazole did not lead to collapse of IF. Instead, the IF formed bundles of coils that seemed to associate with the SF. Disintegration of the SF with cytochalasin B led to the collapse of the IF. It appears that the actin organization in such large cells with extensive SF, is not as contractile as in typical spindle shaped fibroblasts which have relatively less stable actin organization. The stable SF may actually prevent IF collapse.

US$19.90

PMID: 8374596

DOI: 10.1006/cbir.1993.1114


Other references

Nogimori, K.; Menniti, F.S.; Putney, J.W., 1990: Identification in extracts from AR4-2J cells of inositol 1,4,5-trisphosphate by its susceptibility to inositol 1,4,5-trisphosphate 3-kinase and 5-phosphatase. The identity of 3H-labelled material ascribed to Ins(1,4,5)P3 in resting or bombesin-stimulated myo-[3H]inositol-labelled AR4-2J cells was investigated by determining its ability to serve as substrate for partially purified Ins(1,4,5)P3/Ins(1,3,4,...

Fels, E., 1955: Tumor transformations of ovarian grafts into the kidney of female castrate rats. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 149(15-18): 1666-1667

West, C.; Lesellier, E., 2006: Characterisation of stationary phases in subcritical fluid chromatography with the solvation parameter model. III. Polar stationary phases. In this third paper, varied types of polar stationary phases, namely silica gel (SI), cyano (CN)- and amino-propyl (NH2)-bonded silica, propanediol-bonded silica (DIOL), poly(ethylene glycol) (PEG) and poly(vinyl alcohol) (PVA), were investigated...

Gharagozlou, M.J.; Tabatabyi, A.H.; Forci, M., 1992: Pathology of ovine subacute to chronic pneumonia associated with Mycoplasma arginini in Iran. Histological and microbiological studies were made of 88 lungs from a Tehran abattoir and from the Veterinary Medical School PM room. Recorded clinical symptoms had included fever, weight loss and respiratory distress with mucus in the nostrils. G...

Brown, J.Belle.; Bouck, L.Michelle.Sangster.; Østbye, T.; Barnsley, J.M.; Mathews, M.; Ogilvie, G., 2002: Walk-in clinics in Ontario. An atmosphere of tension. To explore family practice (FP), emergency department (ED), and walk-in clinic (WIC) physicians' perceptions and experiences regarding the effect of walk-in clinics on Ontario's health care system. Qualitative method of focus groups. Ham...

Paul, V.; Ekambaram, P.; Jayakumar, A.R., 1998: Effects of sodium fluoride on locomotor behavior and a few biochemical parameters in rats. Spontaneous motor activity and motor coordination were tested in adult female rats after treating with sodium fluoride at 20 or 40 mg/kg dose level daily for 60 days, using an activity chamber and a rota-rod apparatus, respectively. Total protein...

Fauvel, Albert., 1908: Anillobius solifuga, Curculionide aveugle de Madere. Revue d'Entomologie Caen, 261907: 71-72

Rossiter, J.D.; Ellingham, R.; Hakin, K.N.; Twomey, J.M., 2002: Corneal melt and perforation secondary to floppy eyelid syndrome in the presence of rheumatoid arthritis. British Journal of Ophthalmology 86(4): 483-483

Lu, L.; He, K.; Ni, Y.; Yu, Z.; Mao, A., 2017: Exudative epidermitis of piglets caused by non-toxigenic Staphylococcus sciuri. Infections with strains of Staphylococcus sciuri are a potential threat to animal and public health, and a cause for considerable concern. We isolated and identified S. sciuri as a pathogen from an acute outbreak of exudative epidermitis in piglet...

Murphy J.V., 1972: Properties of amniotic fluid hexosaminidases. Pediatric Research 6(4): 363