+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Sarcoplasmic reticulum and myofilament function in chemically-treated ventricular trabeculae from patients with heart failure

, : Sarcoplasmic reticulum and myofilament function in chemically-treated ventricular trabeculae from patients with heart failure. Cardiovascular Research 30(3): 377-385

Objectives: Assessment of sarcoplasmic reticulum calcium-loading ability, myofilament force production and myofilament calcium sensitivity in ventricular trabeculae from patients with heart failure. Methods: Right ventricular trabeculae (diameter 150-250 mu-m) were obtained from 18 patients undergoing elective cardiac transplantation. These were mounted for isometric tension measurement and treated with saponin which permeabilises the sarcolemma leaving the sarcoplasmic reticulum (SR) functionally intact. The trabecula was bathed in a mock intracellular solution containing ATP and weakly buffered (Ca-2+) at various concentrations (150-400 nM). The amplitude of caffeine-induced contractures was used as a quantitative measure of the SR calcium content and was correlated with the clinical severity of heart failure. The same trabecula was then exposed to a solution containing Triton-X100 (1%) which destroys an cell membranes leaving only the myofilaments intact. The maximum calcium-activated force (C-max) and myofilament responsiveness to calcium was assessed. Results: Patients with ischaemic heart disease (IHD) and severe heart failure (PCWP gt 20 mm Hg, ejection fraction lt 15%, n = 8) demonstrated low SR Ca-2+-loading ability compared with patients with IHD and moderate heart failure (PCWP-20 mmHg, LV ejection fraction gt 20%, n = 6). Patients with dilated cardiomyopathy (DCM) (n = 4) demonstrated SR Ca-2+-loading ability which was lower than either of the two IHD groups. Myofilament force production (per unit cross-sectional area) was not significantly different between the three groups. Myofilament responsiveness to Ca-2+ demonstrated no relationship with severity of heart failure. Conclusions: In human heart failure, SR Ca-2+-loading ability diminishes with increasing severity of heart failure. Myofilament force production and sensitivity to calcium are unaffected by severity of heart failure.


PMID: 7585829

DOI: 10.1016/s0008-6363(95)00056-9

Other references

Zlotkin, S.H., 1988: TrophAmine. Pediatrics 82(3): 388-390

Kallapur, S.G.; Willet, K.E.; Jobe, A.H.; Ikegami, M.; Bachurski, C.J., 2001: Intra-amniotic endotoxin: chorioamnionitis precedes lung maturation in preterm lambs. Kallapur, Suhas G., Karen E. Willet, Alan H. Jobe, Machiko Ikegami, and Cindy J. Bachurski. Intra-amniotic endotoxin: chorioamnionitis precedes lung maturation in preterm lambs. Am J Physiol Lung Cell Mol Physiol 280: L527-L536, 2001.--The inflamm...

Lee, C.; Salvatori, R., 2012: Visual vignette. Thyrotroph cell hyperplasia. Endocrine Practice 18(3): 429-429

Glass, K.A.; Kaspar, C.W.; Sindelar, J.J.; Milkowski, A.L.; Lotz, B.M.; Kang, J.; Faith, N.G.; Enache, E.; Kataoka, A.I.; Henry, C., 2012: Validation of pepperoni process for control of Shiga toxin-producing Escherichia coli. The objective of this study was to compare the survival of non-O157 Shiga toxin-producing Escherichia coli (STEC) with E. coli O157:H7 during pepperoni production. Pepperoni batter was inoculated with 7 log CFU/g of a seven-strain STEC mixture, in...

Rigby, M.J.; Robins, S.C., 1997: Building healthcare delivery and management systems centred on information about the human aspects. The importance of modern information systems to manage healthcare delivery at the macro level--particularly through purchasing--is increasingly being recognised. There is also increasing investment in electronic patient record systems to aid care...

Ryzhavskii, B.Ya.; Nikolaeva, I.V.; Uchakina, R.V.; Litvintseva, E.M.; Eremenko, I.P.; Vasil'eva, E.V., 2009: Effect of long-term constant illumination of female rats on the parameters of brain development in their 40-day-old progeny. We studied the progeny from female rats exposed to constant illumination for 1 month and mated with intact males 14 days after this exposure. At the age of 40 days, the progeny from experimental rats differed from the control by lower body weight,...

Wagner, S., 1932: Species crosses in the genus Helianthus. Innumerable interspecific hybrids of Helianthus have been described and many naturally occurring species are regarded as of hybrid origin. The chromosome numbers of the species used were determined and were: H. cucumerifolius, n = 17; H. rigidus,...

Walker, D.M.; Cohen, S.R.; Estrada, F.; Monterroso, M.E.; Jenny, A.; Fritz, J.; Fahey, J.O., 2012: PRONTO training for obstetric and neonatal emergencies in Mexico. To evaluate the acceptability, feasibility, rating, and potential impact of PRONTO, a low-tech and high-fidelity simulation-based training for obstetric and neonatal emergencies and teamwork using the PartoPants low-cost birth simulator. A pilot p...

Gorshkov, Y.G.; Egorov, A.V.; Starunova, I.N.; Zainishev, A.V.; Potemkina, D.V.; Popova, A G., 2006: Fire safety of wheeled agricultural machinery. The fire safety of wheeled machinery in agriculture is briefly outlined. The main causes of fire starting and safety assessments are discussed.

Horvath, J., 1992: Screening of Cucurbita accessions for resistance to different viruses. In tests of 30 accessions of C. moschata, C. ecuadorensis and C. texana and 9 cultivars of C. pepo with 7 viruses, 249 host/virus relations were examined, of which 103 were resistant or immune. Among the 25 accessions of C. moschata, 24 were immun...