geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Stability of protease Q against autolysis and in sodium dodecyl sulfate and urea solutions


, : Stability of protease Q against autolysis and in sodium dodecyl sulfate and urea solutions. Biochemical and Biophysical Research Communications 240(3): 839-843

Protease Q, a recently discovered subtilisin-like protease, exhibited unusual stability in 10% sodium dodecyl sulfate (SDS) and 8 M urea solutions. In 2% SDS it exhibited higher activity than the control, and in 10% SDS it retained 50% of its original activity when azocoll was used as substrate. Kinetic studies showed that the decrease in the activity of protease Q in SDS solutions followed a first order kinetics with a half-life of 446, 278, 78, and 24 h in 1%, 2%, 5%, and 10% SDS, respectively, at 25 degrees C. Protease Q was also stable against autolysis. In 20 mM Tris-HCl buffer (pH 8.3) containing 1.0 mM CaCl2, protease Q had a half life of 2822 h and 725 h at room temperature and at 37 degrees C, respectively. The enzyme was able to completely hydrolyze beta-lactoglobulin, beta-casein, and keratin in 8-10 M urea.

US$19.90

PMID: 9398655

DOI: 10.1006/bbrc.1997.7698


Other references

Muratov, M.M.; Karnaukhov, V.N.; Zinchenko, V.P., 1981: State of cardiac pyridine nucleotides and flavoproteins during preservation with a 0.5% formalin solution. Possibilities of formaldehyde interaction with the respiratory link of mitochondria were studied. For this purpose the fluorescent technique was used to examine pyridine nucleotides and flavoproteins of the conserved heart. The data obtained indic...

Doslak M.J., 1989: Quantitative analysis of the insulating effect of silicone oil on the electroretinogram. A model of the ERG focusing on the insulination effect of silicone oil replacing the vitreous was used to quantify the ERG. The electrical properties of the structures of the eye, the frequency content of the ERG and the percentage of the silicone...

Pang, L.C., 1990: Acute abdominal conditions in mesenteric lymphangioma. Mesenteric lymphangiomas are rare benign tumors that occur in the abdominal cavity attached to the mesentery of the small or large bowel. They are no longer believed to be neoplasms, but may be a result of congenital failure of the original lympha...

Schenkling, S., 1927: Wissenschaftliche Ergebnisse der Bearbeitung der Coleopteren-Sammlung von Franklin Muller. III. Erotylidae Ent Mitt Berlin, 16: 128-129

Nasr, V.G.; Guzzetta, N.A.; Miller-Hance, W.C.; Twite, M.; Latham, G.J.; Zabala, L.; Nicolson, S.C.; Mossad, E.B.; DiNardo, J.A., 2017: Consensus Statement by the Congenital Cardiac Anesthesia Society: Milestones for the Pediatric Cardiac Anesthesia Fellowship. Pediatric cardiac anesthesiology has evolved as a subspecialty of both pediatric and cardiac anesthesiology and is devoted to caring for individuals with congenital heart disease ranging in age from neonates to adults. Training in pediatric cardia...

Doerr, W.; Heider, K.; Reichel, S.; Spekl, K., 2002: Modification of oral mucositis by keratinocyte growth factor Effect of KGF treatment protocol. International Journal of Radiation Oncology Biology Physics 54(2 Supplement): 76

Gudjonsson, H.; Oddsson, E.; Sigurdardottir, M.; Thjodleifsson, B., 1997: Comparison of the efficacy of Losec/Lómex and Zantac/Famex by continuous 24 hour gastric pH-metry.. The study proposes to investigate the pharmacological efficacy of four commonly used acid inhibitory drugs. The effect on 24 hour gastric pH of seven days treatment was assessed for two omeprazole preparations, Losec(R) 20 mg (Hassle) and Lómex(R...

Katano, O.; Nakamura, T.; Yamamoto, S., 2005: Prey fish selection by Far Eastern catfish Silurus asotus and largemouth bass Micropterus salmoides. Prey fish selection by Far Eastern catfish and largemouth bass were examined using bluegill, Japanese dace and crucian carp as food fish. In both aquaria (1.2 m x 0.45 m) and ponds (2.8 m x 1.2 m), bluegill was not preyed on by catfish and bass mo...

Kekwick, R.G.O.; Mackay, M.E.; Martin, N.H., 1953: The preliminary isolation and characterization of human serum cholinesterase. Biochemical Journal 53(4): Xxxvi-Xxxvii

Stock, R., 1986: Risk management: minimizing errors and liability. Dimensions in Health Service 63(1): 22-23