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Stable expression of the human immunodeficiency virus type 1 envelope glycoprotein in transfected L cells


, : Stable expression of the human immunodeficiency virus type 1 envelope glycoprotein in transfected L cells. AIDS Research & Human Retroviruses 8(12): 1999-2009

An SV40-based expression vector was used to generate CD4-negative murine L cell lines which stably expressed the human immunodeficiency virus envelope glycoprotein (env). Despite the presence of abundant intracellular envelope glycoprotein, the expression of env gp120/41 was not detected on the cell surface. Pulse-chase studies showed that the majority of the gp120 detected at the end of a 20-h chase was in the culture medium. Therefore gp120 was shed and/or secreted from these cells. Transfected L cells (H-2-k) served as targets for specific lysis by CTL raised against vaccinia virus-encoded env gp160. The discrepancy in relative levels of intracellular versus surface expression of env was probably due to the highly inefficient processing of newly synthesized gp160, as well as the apparent instability of the gp120/41 complex in the transfected cell lines. Digestion of immunoprecipitated gp120 and gp160 with endoglycosidase H and peptide N-glycosidase F revealed that the envelope glycoprotein in transfected L cells possessed both high mannose and complex N-glycans, analogous to the posttranslational modification of the mature envelope glycoprotein in infected T cells. These studies indicate that the relatively inefficient processing of env gp160 occurs in the absence of CD4, and that the stable surface expression of envelope gp120/41 complex may require additional factors not present in transfected cells.

US$19.90

PMID: 1493050

DOI: 10.1089/aid.1992.8.1999


Other references

Pen, K.H.Ho, W.C., 1932: A preliminary Survey of the Lizards of South-western Szechuan. Journal of the West China Border Research Society, 5: 104-129

Moucka L., 1973: Reflex therapy in the framework of complex balneological therapy in teplice in bohemia. Fysiatricky a Reumatologicky Vestnik 51(1): 53

Droba, M.; Droba, B.; Błedniak, D., 2005: Acid glycosidases from hen oviduct and egg albumen. Activities of seven acid glycosidases: beta -N-acetylhexosaminidase ( beta -HEX), alpha - and beta -galactosidase ( alpha - and beta -GAL), alpha - and beta -mannosidase ( alpha - and beta -MAN), alpha -glucosidase and alpha -fucosidase in magnum...

Grady, H.G., 1955: The pathologic anatomy of cancer of the breast; a summary of the replies to the questionnaire of the International Society of Geographic Pathology. Schweizerische Zeitschrift für Pathologie und Bakteriologie. Revue Suisse de Pathologie et de Bacteriologie 18(4): 685-689

Gordon, E.J., 1976: Special retractors for the hip joint and small bones. Southern Medical Journal 69(8): 1006-1006

Smith J.A.; Shoemaker L.L.; Miller A.J., 1984: Transport and storage of sediment in the Potomac River basin. Eos, Transactions, American Geophysical Union 65(16): 218

Pribaz, J.J.; Weiss, D.D.; Mulliken, J.B.; Eriksson, E., 2000: Prelaminated free flap reconstruction of complex central facial defects. This article is a review of five patients who underwent reconstruction of nasal and paranasal facial defects with prelaminated forearm free flaps. The defects resulted from thermal injury, gunshot wound, excision of tumor, and arteriovenous malfor...

Arcy, L.B.; Scudamore, H.H., 2011: Correlated defects in human agnathus. Anatomical Record 94: 414-414

Gomes C.D.Sousa Figueredo, 1985: The effects of mineralizers intercalated in the kaolinite structure, on the high temperature phases. Mineralogica et Petrographica Acta 29(A): 381-390

Morrison, N.; Simpson, C.; Fothergill-Gilmore, L.; Boyd, E.; Connor, J.M., 1992: Regional chromosomal assignment of the human platelet phosphofructokinase gene to 10p15. A cDNA for human platelet 6-phosphofructokinase (PFKP) has been isolated from a human Raji cell line cDNA library. Using this cDNA as a probe, the gene for human PFKP, previously mapped to chromosome 10pter-p11.1, has been further localized to 10p...