+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Structural similarity between lung surfactant protein D and conglutinin: Two distinct, C-type lectins containing collagen-like sequences

, : Structural similarity between lung surfactant protein D and conglutinin: Two distinct, C-type lectins containing collagen-like sequences. European Journal of Biochemistry 215(3): 793-799

Preparations of bovine lung surfactant D (SP-D) and conglutinin were examined by electron microscopy, gel-filtration and SDS/PAGE. SP-D is composed of non-covalently linked subunits, of 160 kDa, which each contain three, disulphide-linked, 44-kDa polypeptide chains. In the electron microscope a single 160-kDa subunit of SP-D appears as a 45.8 +- 3-nm-long rod connected to a small globular 'head'. Particles were also seen which correspond to non-covalently linked dimers, trimers and tetramers of the 160-kDa monomer subunit of SP-D. The tetramer structure contains 12 polypeptide chains and is very similar to the electron microscopy images and model reported by Strang et al. (Strang, C. J., Slayter, US., Lachmann, P. J. and Davis, A. E. (1986) Biochem. J. 236, 3811-389) for bovine conglutinin in which four 160-kDa subunits are disulphide-linked to give a molecule of expected molecular mass of 528 kDa. This study confirmed the findings by Strang et al. in the above paper for intact conglutinin and also emphasised that the rod-like structures, of length 37.6 +- 3.7 nm, seen in the conglutinin subunits were significantly shorter than those in SP-D despite the close similarity in amino acid sequence (79% identity) and chain length between the two proteins. In addition, a truncated form of conglutinin was found in the conglutinin preparations, due to limited proteolysis of the Arg-Ala bond at position 54 in the 44-kDa chains. These truncated conglutinin chains yield a subunit composed of three shortened, non-disulphide-linked, chains and this subunit appears as a monomer with a rod length of 34.2 +- 2.8 nm in the electron microscope. On gel-filtration, a proportion of the SP-D preparation behaved, as expected, as a molecule with an apparent molecular mass of 600 kDa. The remainder of the SP-D preparation behaved as aggregated material with a molecular mass greater than 900 kDa which yielded no distinct structures in the electron microscope. Intact conglutinin was eluted at a position greater than 900 kDa but yet provided clear electron microscopy images of the tetramer structure described above. Although it is difficult to explain fully the anomolous behaviour of SP-D and conglutinin on gel-filtration, it is proposed that the natural form of both SP-D and conglutinin is the highest distinct oligomer form seen in the electron microscope, i.e. a tetramer of the 160-kDa subunits in which the four rod-like elements are linked in a tail-to-tail fashion to a central core.


PMID: 8354286

DOI: 10.1111/j.1432-1033.1993.tb18094.x

Other references

Cullen, N.G.; Morris, C.A.; Hickey, S.M., 2006: Genetic parameters for resistance to facial eczema in dairy cattle. A project was designed to progeny-test dairy industry sires for their resistance to facial eczema (FE), as a preliminary step towards identifying DNA markers or genes for FE resistance. The FE disease is caused by the toxin, sporidesmin, produced...

Cai, W.; Zhao, H.; Yuan, Z., 2005: Roles of glial cell line-derived neurotropic factor in motoneuron development and motoneuron disease. To review research progress of the relation between glial cell line-derived neurotropic factor (GDNF) and motor neuron development and motor neuron disease. The recent articles on GDNF and motor neurons were extensively reviewed. The molecular str...

Krabatsch, T.; Fleck, E.; Hetzer, R., 1995: Treating poststernotomy mediastinitis by transposition of the greater omentum: late angiographic findings. Transposition of the greater omentum to the thorax for treatment of severe mediastinal infections due to the anatomical structure and physiological properties of the omental tissue is a proven and safe procedure. From 1987 to 1993, thoracic omento...

Polishuk, Z.; Sharf, M.; Kuzminski, T., 1961: Induction of ovulation by intravenous estrogens. The induction of ovulation by large doses of estrogens was studied in 53 cases with anovulatory cycles or amenorrhea. The cases were divided into three groups: Group I consisted of 21 cases with anovulatory cycles, showing signs of estrogen secret...

Khokhar, S.S.; Wirth, T., 2004: Selenocyclizations: control by coordination and by the counterion. Angewandte Chemie 43(5): 631-633

Jones, M.J.; Shanley, T.P.; Kapur, V.; Musser, J.M.; Ward, P.A., 1994: In vivo augmentation of pulmonary injury by streptococcal pyrogenic exotoxin B-conversion of Pre-IL-1-beta to functionally active IL-1-beta. Journal of Leukocyte Biology 0(SUPPL ): 37

Shimizu, H.; Satoh, A.K.; Kawamura, S.; Ozaki, K., 2000: Two distinct roles of RAB5 in the vesicle transport system of the Drosophila photoreceptor cell. Zoological Science (Tokyo) 17(Supplement): 97, December

Angeli, L., 1957: Yield trials with heterotic red pepper. At the Horticultural and Viticultural College, Hungary, it was found that hybrids ripen earlier than the parent plants, surpass them in yield and are more resistant to diseases. Hybrid seed production is, however, uneconomical since fruit set in c...

Kumral, A.; Tuzun, F.; Ozbal, S.; Ugur Ergur, B.; Yılmaz, O.; Duman, N.; Ozkan, H., 2013: Lipopolysaccharide-preconditioning protects against endotoxin-induced white matter injury in the neonatal rat brain. Exposing the brain to a sub-damaging stimulus can protect against a subsequent lethal insult, a phenomenon termed preconditioning. The aim of this study was to investigate the neuroprotective effect of low dose LPS (lipopolysaccharide) pretreatmen...

Macintyre, S., 1997: The Black Report and beyond: what are the issues?. This paper provides an overview of the Black Report, published in Britain in 1980. It outlines its place in the history of British concern about socio-economic differentials in death rates since the mid-19th century, and suggests continuities in s...