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Studies of the mechanism for enhanced cell surface factor VIIa/tissue factor activation of factor X on fibroblast monolayers after their exposure to N-ethylmaleimide


, : Studies of the mechanism for enhanced cell surface factor VIIa/tissue factor activation of factor X on fibroblast monolayers after their exposure to N-ethylmaleimide. Thrombosis and Haemostasis 72(6): 848-855

Fibroblast monolayers constitutively expressing surface membrane tissue factor (TF) were treated with 0.1 mM N-ethylmaleimide (NEM) for 1 min to inhibit aminophospholipid translocase activity without inducing general cell damage. This resulted in increased anionic phospholipid in the outer leaflet of the cell surface membrane as measured by the binding of 125I-annexin V and by the ability of the monolayers to support the generation of prothrombinase. Specific binding of 125I-rVIIa to TF on NEM-treated monolayers was increased 3- to 4-fold over control monolayers after only brief exposure to 125I-rVIIa, but this difference progressively diminished with longer exposure times. A brief exposure of NEM-treated monolayers to rVIIa led to a maximum 3- to 4-fold enhancement of VIIa/TF catalytic activity towards factor X over control monolayers, but, in contrast to the binding studies, this 3- to 4-fold difference persisted despite increasing time of exposure to rVIIa. Adding prothrombin fragment 1 failed to diminish the enhanced VIIa/TF activation of factor X of NEM-treated monolayers. Moreover, adding annexin V, which was shown to abolish the ability of NEM to enhance factor X binding to the fibroblast monolayers, also failed to diminish the enhanced VIIa/TF activation of factor X. These data provide new evidence for a possible mechanism by which availability of anionic phospholipid in the outer layer of the cell membrane limits formation of functional VIIa/TF complexes on cell surfaces.

US$29.90

PMID: 7740453


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Madrigal, C., A., 1983: New species of lepidopterous defoliators attacking the conifers in Colombia. Revista colombiana de entomologia (pub 1983) 7(3-4): 3-14

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Es, A. van; Hartmans, K.J., 1987: Dormancy, sprouting and sprout inhibition. This chapter covers all aspects of dormancy, sprouting and sprout inhibition in stored potatoes, including the effect of tuber size and age; cv.; disease; storage temp.; moisture, O2, CO2 and volatile substances content of the storage atmosphere o...

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Benda, N.; Gambelunghe, C., 1962: Blood groups and tumors. La Riforma Medica 76: 266-268

Perelman R.H.; Farrell P.M., 1982: Analysis of causes of neo natal death in the usa with specific emphasis on fatal hyaline membrane disease. National mortality statistics for hyaline membrane disease (HMD) and the respiratory distress syndrome (RDS) and other major causalities were examined for 1968-1978. A progressive reduction in total neonatal deaths began in 1971 such that only 56%...

Sussman, E.S.; Gumenyuk, V., 2005: Organization of sequential sounds in auditory memory. A repeating five-tone pattern was presented at several stimulus rates (200, 400, 600, and 800 ms onset-to-onset) to determine at what temporal proximity the five-tone repeating unit would be represented in memory. The mismatch negativity component...

Ternovoĭ, S.K., 1981: Indications for computerized axial x-ray tomography in the diagnosis of pathological processes of the abdominal cavity and retroperitoneal space. Terapevticheskii Arkhiv 53(2): 40-44

Lalancette M F.; Standing L., 1990: Asch fails again. The social conformity paradigm of Asch (1956) was modified to make the test stimuli more ambiguous, and thus to increase the likelihood of obtaining conformity. With the same aim, both anonymous and individuated conditions were used. As with a pre...