geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Survival and dispersion of genetically modified rhizobia in the field and genetic interactions with native strains


, : Survival and dispersion of genetically modified rhizobia in the field and genetic interactions with native strains. FEMS Microbiology Ecology 15(1-2): 147-159

A genetically modified strain of the symbiotic nitrogen-fixing bacterium Rhizobium leguminosarum biovar viciae was used to inoculate a typical host, pea, and a control non-host cereal crop in the field. The inoculant was monitored for survival and spread from the site of application, and for genetic interactions with the native population. It could be identified by chromosally located antibiotic resistance markers and additional markers conferred by the transposon Tn5 inserted on its conjugative symbiotic plasmid. These markers facilitated enumeration of the strain on selective agar, enabling survival and spread to be monitored over a six year period. Although culturable cell numbers dropped two to three orders of magnitude after the first year, subsequently they remained around 10-2 viable cells per g soil, even in subplots where only the non-host cereals had been grown. However, peas did give the inoculant a small survival advantage compared with non-hosts. Soil cultivation appeared to play a major role in inoculant dissemination from the site of application. Transfer of the Tn5 marker to other rhizobia could be monitored by screening for isolates with Tn5-encoded antibiotic resistance in the absence of the inoculant chromosomal markers. Over three years, more than 4000 pea root nodules were screened for indigenous rhizobia that had acquired the Tn5-marked symbiotic plasmid from the inoculant. None were detected, although overall about 2% of nodules contained the inoculant strain, and transfer of the Tn5-marked symbiotic plasmid to three out of four R. leguminosarum biovar viciae isolates from the field site could be demonstrated under laboratory conditions.

US$19.90

DOI: 10.1111/j.1574-6941.1994.tb00239.x


Other references

Yanai K.; Watanabe T., 1991: Age related decrease in histamine h 1 receptor in human brain measured with positron emission tomography pet discrepancy between pet and in vitro binding study. Neuroscience Research Supplement (16): S100

Prasad, A.S.; Beck, F.W.J.; Bao, B.; Fitzgerald, J.T.; Snell, D.C.; Steinberg, J.D.; Cardozo, L.J., 2007: Zinc supplementation decreases incidence of infections in the elderly: effect of zinc on generation of cytokines and oxidative stress. Background: Zinc deficiency, cell-mediated immune dysfunction, susceptibility to infections, and increased oxidative stress have been observed in elderly subjects (ie, those > 55 y old). Zinc is an effective antiinflammatory and antioxidant ag...

Frost, R.L.; Makó, E.; Kristóf, J.; Kloprogge, J.T., 2002: Modification of kaolinite surfaces through mechanochemical treatment--a mid-IR and near-IR spectroscopic study. The modification of kaolinite surfaces through mechanochemical treatment has been studied using a combination of mid-IR and near-IR spectroscopy. Kaolinite hydroxyls were lost after 10 h of grinding as evidenced by the decrease in intensity of the...

Taylor, M.M.; Stokes, W.S.; Bajuscak, R.; Serdula, M.; Siegel, K.L.; Griffin, B.; Keiser, J.; Agate, L.; Kite-Powell, A.; Roach, D.; Humbert, N.; Brusuelas, K.; Shekar, S.S., 2007: Mobilizing mobile medical units for hurricane relief: the United States Public Health Service and Broward County Health Department response to hurricane Wilma, Broward County, Florida. Journal of Public Health Management and Practice 13(5): 447-452

Harrisson F.; Van Hoof J., 1985: Fibronectin in epithelial sheet migration on the basement membrane of the chicken epiblast. Cell Differentiation 16(SUPPL): 171S

Melvin, D.M., 1950: The life-cycle of Monoecocestus sigmodontis (Cestoda : Anoplocepha-lidae) from the cotton rat (Sigmodon hispidus). Oribatid mites are intermediate hosts of Monoecocestus sigmodontis. Mature cysticercoids were found in six species of oribatids seven to eight weeks after feeding on the eggs of M. sigmodontis from infected cotton-rats. Eggs appeared in the faeces...

Della Santa, D.; Chamay, A., 1995: Radiological evolution of the rheumatoid wrist after radio-lunate arthrodesis. Radio-lunate arthrodesis of the rheumatoid wrist is an established technique which has been in use for more than 12 years. The evolution of 26 operated wrists and 20 non-operated wrists has been studied with a mean follow-up of 5 years. The result...

Regan, D., 1968: Chromatic adaptation and steady-state evoked potentials. Steady-state scalp potentials were evoked by stimulating the eye with monochromatic light whose intensity was sinusoidally modulated at frequencies between 4 cycles/sec, and 33 c/s. The modulated beam was superposed on an adapting bean whose color...

Salama, S., 2013: Cutaneous collagenous vasculopathy. Journal of Cutaneous Pathology 40(5): 524-524

Heyman, M.B.; Branski, D., 2012: Commentaries on "Workshop report: developing a pediatric inflammatory bowel diseases network and data platform in Canada": international networking in pediatric gastroenterology research: advancing science and medicine with collaborative efforts. Journal of Pediatric Gastroenterology and Nutrition 55(2): 121-121