geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The activity of the pseudorabies virus latency-associated transcript promoter is dependent on its genomic location in herpes simplex virus recombinants as well as on the type of cell infected


, : The activity of the pseudorabies virus latency-associated transcript promoter is dependent on its genomic location in herpes simplex virus recombinants as well as on the type of cell infected. Journal of Virology 68(3): 1972-1976

As do many other alphaherpesviruses, pseudorabies virus (PRV) transcribes a limited portion of its viral genome in latently infected neurons during latency. The sequence of the PRV latency-associated transcript (LAT) is bounded on its 5' end by a putative promoter region which contains sequence elements similar to those characterized for the herpes simplex virus (HSV) LAT promoter. Using the bacterial beta-galactosidase gene as a reporter, we have assayed PRV LAT promoter activity in the genomic environment in recombinant HSVs. The PRV LAT promoter-beta-galactosidase reporter gene was recombined into the terminal and internal long repeat regions (R(L) regions), replacing the normal HSV LAT promoter, the cap site, and the first 60 bases of the primary transcript. When recombined into the R(L) region, appreciable reporter gene expression was observed following infection of two cell lines of neuronal origin; little or no activity was seen with these recombinants following infection of rabbit skin or mouse embryo fibroblasts. No significant expression was seen when the promoter was recombined into the gC locus in the long unique region in any of the cell types utilized. Such results suggest that the PRV latency promoter contains neuronal cell-specific elements and that the HSV R(L), region provides an appropriate genomic environment for the manifestation of that specificity.

US$29.90

PMID: 8107257


Other references

Defalque, R.J.; Stoelting, V.K., 1967: Latency and duration of action of common local anesthetics: an experimental evaluation. Anesthesia and Analgesia 46(3): 311-314

Yavtushenko, V.E.; Makarov, N.B., 2001: Agrochemical properties of eroded soils in Russia and use of fertilisers for agricultural production. Analysis of published data on the state of eroded soils in Russia was conducted. Data on granulometric composition of eroded soils, their physical and hydrological properties, humus and nitrogen content, pH, mineral elements contents, losses of hu...

Gaiger, S.H.; Davies, G. 0., 1933: Brucella abortus in cow's milk. Of 200 samples of milk submitted to the biological test for tuberculosis, 43.5% were shown to have generated Br. abortus agglutinins in the inoculated guinea pigs, as determined by agglutination tests with sera of guinea pigs collected in the 6th...

Cassiolato, A.M.ria R.; Baker, R.; Melo, I.S.D., 1997: Action of Trichoderma harzianum mutants on the formation and carpogenic germination of Sclerotinia sclerotiorum sclerotia and lettuce plant survival. The objective of this research was to evaluate the antagonistic ability of benomyl-resistant mutants of Trichoderma harzianum (TW5 and WT-T95) against mycelial growth, sclerotia formation, carpogenic germination of sclerotia of Sclerotinia sclerot...

Hagiwara Y.; Tajima H.; Izutuya S.; Hanada H., 1977: Gravity change associated with earthquake swarm activities in the eastern part of Izu Peninsula. Bulletin of the Earthquake Research Institute = Tokyo Daigaku Jishin Kenkyusho Iho 52(2): 141-150

Jiang, S.; Zhao, L.; Purandare, B.; Hantash, B.M., 2010: Differential expression of stem cell markers in human follicular bulge and interfollicular epidermal compartments. Although skin contains a number of stem cell repositories, their characterization has been hindered by a lack of specific markers and an unclear in vivo localization. In this study, we whole mounted single human scalp hair follicles and examined t...

Nathanson, I.; Ouellette, D.R., 2015: Point: Are the CHEST guidelines global in coverage? Yes. Chest 147(1): 11-13

Abrams, S., 1978: The ceiling tiles. Psychoanalytic Quarterly 47(1): 116-117

Cohen, E.L.rie, S.S.apiro, B.B.n-Yehoshua, S.S.alom, Y.R.senberger, I., 1990: Prolonged storage of lemons using individual seal-packaging. 'Eureka' lemons [Citrus limon (L.) Burm. f.] treated for commercial storage were held for 6 months at 13C. One-half of the fruits were individually sealed in high-density polyethylene (HDPE) plastic film and half not sealed. The HDPE-sea...

D.Pasquale G., 1969: Uterotrophic activities of various estrogens after topical application to the rat abstract. Federation Proceedings: 703