geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The developmental ability of human oocytes penetrated at the germinal vesicle stage after insemination in vitro


, : The developmental ability of human oocytes penetrated at the germinal vesicle stage after insemination in vitro. Human Reproduction 9(4): 697-708

This study demonstrates that sperm penetration into the ooplasm occurs at high frequency in germinal vesicle (GV) stage human oocytes which failed to resume meiosis after ovulation induction in cycles of ovarian hyperstimulation for in-vitro fertilization. The capacity of the immature human oocyte to prevent polyspermic penetration at the cell surface level was suggested by the finding that despite the presence of numerous spermatozoa within the zona pellucida and on the oocyte surface within 3 h after insemination, all normal-appearing GV stage oocytes examined in this study were penetrated by a single spermatozoon. This notion was also supported by scanning confocal microscopic analysis of oocytes double-stained for DNA and cortical granules which showed highly localized regions of cortical granule-free cytoplasm in proximity to the penetrated spermatozoon. The developmental ability of these oocytes was assessed by culture in vitro. The results show that oocytes penetrated by a spermatozoon at the GV stage resume meiosis, develop the capacity to decondense sperm DNA, abstrict both first and second polar bodies, and form a male pronucleus from. the spermatozoon which enters the oocyte prior to the resumption of meiotic maturation. After penetration, sperm nuclei rapidly migrate to the centre of the oocyte and become juxtaposed with the germinal vesicle, suggesting the presence of a cellular mechanism which permits directed movement within the cytoplasm. The developmental ability of these oocytes and the normality of the resulting embryos are discussed.

US$29.90

PMID: 8046026


Other references

Brajkovic, D.; Ducharme, M., B., 2004: Cheek skin temperature and thermal resistance in active and inactive individuals during exposure to cold wind. 1. The present study examined the effect of the thermal state of the body (as reflected by rectal temperature) on cheek skin temperature and thermal resistance in active and inactive subjects. 2. Active subjects were exposed to a 30 min conditioni...

Nishioka, K.; Fujimoto, M.; Date, R.; Masuda, Y.; Hiramato, K.; Tanaka, T., 1984: Bilateral sensorineural deafness associated with Mycoplasma pneumoniae infection: the first case report. Hiroshima Journal of Medical Sciences 33(4): 585-589

Lapham, G.; Gaillard, W.Davis.; Sexter, J.; Berl, M.M., 2017: Increasing Awareness of Sudden Death in Pediatric Epilepsy Together. Pediatrics 139(2)

Halmagyi, D.; Robicsek, F.; Felkai, B.; Ivanyi, J.; Szoter, T.; Szucs, Z., 1955: Experimentally induced chronic insufficiency of the right heart in dog. Magyar Belorvosi Archivum 8(3): 80-84

Pereira R.M.; Wilhelm C.F.; Vieira M.C.; de Oliveira R.; Pimentel M.B., 1986: Note on chromite exploration in the Serra de Bom Sucesso and the Serra de Ibituruna, Minas Gerais. Anais da Academia Brasileira de Ciencias 58(2): 267-275

O'Connell, P.J.; Ascher, N.; Delmonico, F.L., 2016: The Transplantation Society Believes a Policy of Engagement Will Facilitate Organ Donation Reform in China. American Journal of Transplantation 16(11): 3297-3298

Cuffney, T.F.; Zappia, H.; Giddings, E.M.; Coles, J.F., 2005: Effects of urbanization on benthic macroinvertebrate assemblages in contrasting environmental settings: Boston, Massachusetts; Birmingham, Alabama; and Salt Lake City, Utah. American Fisheries Society Symposium, 47: 361-407

W.Z.iyong; Jiang Yanwen, 1996: A brief introduction for quantitative dynamic stratigraphy. The quantitative dynamic stratigraphy (QDS) is a new synthetic subject which studies the geologic dynamics, stratigraphy, sedimentology and hydrodynamics of a sedimentary basin by using quantitative mathematical methods, and finally obtains the di...

Ma, Y.B., 1993: Accelerating agricultural development following the rules of a market economy. Development of the market economy in agriculture requires close regulation of the structure of agricultural production and the development of high yields of good quality produce. Development needs to be carried out with care. For each improvement...

Williams, C.R., 1972: The value of frusemide in 110 consecutive prostatectomies. British Journal of Surgery 59(3): 190-194