+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The effects of copper on actin and fibronectin organization in Mytilus galloprovincialis haemocytes

, : The effects of copper on actin and fibronectin organization in Mytilus galloprovincialis haemocytes. Developmental and Comparative Immunology 20(6): 383-391

The effects of copper on actin and fibronectin organization in Mytilus gallo-provincialis haemocytes were studied. The Cu-2+ exposure of mussels caused severe perturbations in haemocyte actin and fibronectin organization with respect to non-exposed organisms. Cytoskeletal actin was analysed by indirect immunofluorescence, using an anti-total actin monoclonal antibody, and by rhodamine-conjugated phalloidin. The majority of haemocytes from Cu-2+-exposed mussels displayed a round morphology, with short and blunt filopodia; they lacked the polarized phenotype which was typical in control samples. The cytoskeleton alteration, more evident after phalloidin staining, resulted in the disappearance of filamentous actin. The actin cortical meshwork also appeared disorganized. The cytoskeletal morphology studied by transmission electron microscopy after negative staining of Triton X-100-treated haemocytes confirmed these observations. The structural organization of actin when analysed by Western blotting showed a larger number of Triton-soluble actin pools in treated mussel haemocytes. Fibronectin was studied by indirect immunofluorescence using a polyclonal antiserum directed against mussel fibronectin. In treated mussels, fibronectin appeared to be strongly disorganized and its levels decreased in both haemocytes and haemolymph. The mechanism(s) of the copper-induced alterations on actin and fibronectin organization in mussel immunocytes is discussed.


PMID: 9040981

Other references

Salzberg, B.M.; Kosterin, P.V.; Muschol, M.; Obaid, A.L.; Rumyantsev, S.L.; Bilenko, Y.; Shur, M.S., 2004: An ultra-stable non-coherent light source for optical measurements in neuroscience and cell physiology. We demonstrate that high power light-emitting diodes (LED's) exhibit low-frequency noise characteristics that are clearly superior to those of quartz tungsten halogen lamps, the non-coherent light source most commonly employed when freedom fr...

Hall T.C., 1988: Organ toxicities and antitumor responses are they pharmacogenetically related. Hacker, M P , J S Lazo And T R Tritton (Ed ) Developments in Oncology: Organ Directed Toxicities Of Anticancer Drugs; First International Symposium, Burlington, Vermont, Usa, June 4-6, 1987 Xii+254p Kluwer Academic Publishers: Dordrecht, Netherlands; Boston, Massachusetts, Usa Illus 248

Kostellow A.B.; Morrill G.A., 1977: Role of calcium and cyclic nucleotides in progesterone induced meiotic maturation in rana pipiens. Federation Proceedings 36(3): 389

Qiao, J.; Hou, X.; Steier, P.; Golser, R., 2013: Sequential injection method for rapid and simultaneous determination of 236U, 237Np, and Pu isotopes in seawater. An automated analytical method implemented in a novel dual-column tandem sequential injection (SI) system was developed for simultaneous determination of (236)U, (237)Np, (239)Pu, and (240)Pu in seawater samples. A combination of TEVA and UTEVA ex...

French A.V.; O'rourke P.K.; Cameron D.G., 1988: Rotational and continuous grazing of zulu forage sorghum sorghum spp hybrid by beef cattle grazed at 3 stocking rates. A grazing trial, using the hybrid forage sorghum (Sorghum spp. hybrid) cultivar Zulul, compaerd rotational grazing (a 4 week cycle, 2 weeks in each of 2 half-paddocks) with conventional continuous grazing at each of 3 stocking rates. 1.92, 2.85 an...

Novosad, F.I., 1996: Prognostic value of total IgE and IgE in circulating immune complexes contents in bronchial asthma patients during immunosorption. European Respiratory Journal Supplement 9(23): 210S

Reiche, C., 1995: PIXEL: experiences with the element. Studies in Health Technology and Informatics 29: 681-689

Anonymous, 1980: Tests with the Juko combination machine model 1978 for sowing commercial fertilizer and grain, plus use of model 1980. Melding Landbruksteknisk institutt: 80 (469)

de Araújo, V.C.; de Sousa, S.O.; Carvalho, Y.R.; de Araújo, N.S., 2000: Application of immunohistochemistry to the diagnosis of salivary gland tumors. A panel of antibodies composed of the cytokeratins (CKs), vimentin, and actin was applied to 114 minor salivary gland tumors to evaluate its diagnostic value. The results revealed that luminal cells of intercalated duct-like structures, such as th...

Inaba, Y.; Bergmann, S.R., 2010: Prognostic value of myocardial metabolic imaging with BMIPP in the spectrum of coronary artery disease: a systematic review. We conducted a systematic review to summarize the current literature on the prognostic value of BMIPP imaging, fatty-acid metabolic imaging, for the prediction of cardiovascular events in coronary artery disease. Electronic databases (including Ja...