+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The pericentriolar lattice of PtK2 cells exhibits temperature and calcium-modulated behavior

, : The pericentriolar lattice of PtK2 cells exhibits temperature and calcium-modulated behavior. Journal of Cell Science 107: 2993-3003

In this study, we demonstrate that manipulations of temperature and free calcium alter the morphology of the centrin-containing pericentriolar lattice of PtK-2 cells. Immunofluorescence microscopy reveals that low-temperature incubation (4 degree C) causes anti-centrin-labeled pericentrosomal spots to coalesce in the peripheral cytoplasm, and fuses small spots into larger spots near the cell center. At electron microscopic resolution, well-formed pericentriolar satellites appear around the centrioles in response to incubation at 4 degree C. Elevated free calcium enhances these low-temperature-dependent effects. The data suggest that pericentrosomal spots correspond to one or more pericentriolar satellites, and that pericentriolar satellites and centrosomal matrix are interconvertable forms of the same material. Transient elevation of intracellular free calcium at 37 degree C from a basal level of 3.7 times 10-8 M to a peak level of 2.0 times 10-7 M within 30 seconds with ionomycin results in a 35% increase in pericentrosomal spot number throughout the cytoplasm. The number of pericentrosomal spots is 50% larger 2 minutes after ionomycin addition; these spots are also nearer to the cell center as compared to 30 seconds after ionomycin addition. As intracellular free calcium returns to a basal level over 5 minutes, the number of spots and their cellular distribution resume a pretreatment value and pattern. We interpret these observations to indicate movement of pericentrosomal spots toward the cell center in response to the flux in intracellular free calcium. Alternatively, it is possible that no movement has occurred, but that the rise in free calcium has unmasked an epitope responsive to our anti-centrin antiserum. Regardless of the interpretation, we conclude that the pericentriolar lattice exhibits calcium-modulated behavior.


PMID: 7698999

Other references

Song, K.S.; Kim, H.K.; Song, J.W.; Choi, J.R.; Park, Y.S., 2001: A novel silent substitution (C8516T) in exon 9 of the human PROC gene. Protein C is a vitamin K dependent serine protease zymogen, which has a regulatory influence over the coagulation cascade via the inhibition of factors Va and VIIIa. Hereditary protein C deficiency is associated with an increased risk of thromboem...

Johnson M.G.; Malgady R.G., 1979: Some cognitive aspects of figurative language association and metaphor. The relationship between some predictions generated by a cognitive-feature model of association and metaphor understanding was studied. Data were collected from University of Tennessee undergraduates on the 2 key nouns in each of 28 randomly selec...

Schindler Michael; Huminicki Danielle M.C.; Hawthorne Frank C., 2000: A bond-valence approach to the structure and chemistry of sulfate minerals. We introduce a new crystal-chemical approach to the structure and composition of the sulfate minerals. The structure of a mineral is divided into two parts: (1) the structural unit, which is defined by the presence of strong bonds between the cons...

Michel Franz, 1974: Contributions to the osteology of the marmots; 4, The form of the humerus, especially of the foramen entepicondyloideum, and its changes from Pleistocene to the present in Marmota marmota. Mitteilungen der Naturforschenden Gesellschaft in Bern 31: 25-48

Roodman, R.D., 1980: Physicians' office condos--a unique approach to capital development. Hospital Financial Management 34(9): 20, 24-6

Soltani, S.; Zakeri-Milani, P.; Barzegar-Jalali, M.; Jelvehgari, M., 2016: Design of eudragit RL nanoparticles by nanoemulsion method as carriers for ophthalmic drug delivery of ketotifen fumarate. Ketotifen fumarate (KF) is a selective and noncompetitive histamine antagonist (H1-receptor) that is used topically in the treatment of allergic conditions of rhinitis and conjunctivitis. The aim of this study was to formulate and improve an ophth...

Boroń, P.; Borzuchowska, A., 1969: Enzymatic and histological changes in the liver of rats after experimental adrenalectomy. Polish Medical Journal 8(6): 1391-1395

Pirtle, O.L., 1994: X-ray machine calibration: a study of failure rates. Inaccurate calibrations can seriously affect the technique chart and, ultimately, affect patient exposure and the diagnostic quality of radiographs. Texas regulations require that several quality control tests, including kV accuracy, timer accurac...

Smith, H.L J.R., 1949: Thermal compression evaporation-its application to the concentration of waste sulphite liquor. Presents calculations for the design of a commercial thermal-compression evaporating unit of sufficient capacity to concentrate a plant's entire output of 10% solid sulphite liquor.

Cassidy, M.K.; Houston, J.B., 1980: Phenol conjugation by lung in vivo. Biochemical Pharmacology 29(3): 471-474