geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Three-dimensional microcomputed tomography of renal vasculature in rats


, : Three-dimensional microcomputed tomography of renal vasculature in rats. Hypertension 31(1 Pt 2): 440-444

Current microscopic methods to view renal microvasculature reveal only a very limited portion of the total renal volume. Identification of connectivity for postglomerular vessels in the cortex and the medulla during functional states related to changes in sodium excretion will help better to understand the coupling of renal vasculature to tubular function. The purpose of this study was to investigate the possibility of visualizing the continuity of pre- and postglomerular vasculature using three-dimensional micro-computed tomography (micro-CT). Kidneys from normal rats were perfusion fixed in situ at physiological pressure, filled with latex microfil containing lead chromate, and embedded in plastic. The micro-CT scans of the intact kidneys were carried out on a rotating stage illuminated either by a synchrotron x-ray source or a conventional x-ray spectroscopy tube. Images were reconstructed by a filtered backprojection algorithm and volume-rendering techniques were utilized to display the vasculature. The reconstructed images clearly showed the large distribution vessels and the venous drainage of the kidneys, while pre- and postglomerular vessels and their vascular connections throughout the kidney were displayed in great detail. Efferent arterioles showed the characteristics of their peritubular capillary beds in the cortical and medullary regions. The vascular volume of the cortex was 27%, the outer stripe of the outer medulla 18%, the inner stripe of the outer medulla 30%, and the inner medulla 18%. In conclusion, micro-CT is a promising method to evaluate renal vascular architecture relative to physiological and pathological alterations.

US$19.90

PMID: 9453342

DOI: 10.1161/01.hyp.31.1.440


Other references

Marais, M.; Kruger, J.C. de W., 1991: The cytogenetics of some South African root-knot nematodes (Heteroderidae: Nematoda). The cytogenetics of six South African Meloidogyne species were studied. Some interspecific differences were noted during oogenesis as well as interspecific and intraspecific differences in chromosome numbers of the various populations.

Cintas, L.M.; Casaus, P.; Håvarstein, L.S.; Hernández, P.E.; Nes, I.F., 1997: Biochemical and genetic characterization of enterocin P, a novel sec-dependent bacteriocin from Enterococcus faecium P13 with a broad antimicrobial spectrum. Enterocin P is a new bacteriocin produced by Enterococcus faecium P13 isolated isolated from a Spanish dry-fermented sausage. Enterocin P inhibited most of tested spoilage and food-borne gram-positive pathogenic bacteria, such as Listeria monocyto...

Unger E.F.; Sheffield C.D.; Epstein S.E., 1988: Effect of heparin on the angiogenesis response to experimental myocardial ischemia. Journal of the American College of Cardiology 11(2 SUPPL A): 253A

Mitra, S.P.; Prakash, D., 1957: Adsorption of potassium as influenced by concentration and pH of the solution. Montmorillonite shows the greatest adsorption at all pH values. Adsorption varies with the concentration of K in the solution, pH of the solution and nature of the cations in the exchange complex. Data are presented for kaolinite, montmorillonite,...

Giuffrida, A.; Gravelle, H.; Roland, M., 1999: Measuring quality of care with routine data: avoiding confusion between performance indicators and health outcomes. Objective To investigate the impact of factors outside the control of primary care on performance indicators proposed as measures of the quality of primary care. Design Multiple regression analysis relating admission rates standardised for age and...

Guido Ventura; Giuseppe Vilardo, 1999: Seismic-based estimate of hydraulic parameters at Vesuvius Volcano. Geophysical Research Letters 26(7): 887

Koivula, T.; Koivusalo, M., 1975: Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution. 1. The properties and distribution of the NAD-linked unspecific aldehyde dehydrogenase activity (aldehyde: NAD+ oxidoreductase EC 1.2.1.3) has been studied in isolated cytoplasmic, mitochondrial and microsomal fractions of rat liver. The various t...

Knezevic, Z.; Gosak, D.; Hraste, M.; Jalsenjak, I., 1998: Fluid-bed microencapsulation of ascorbic acid. Ascorbic acid has been fluidized and microencapsulated with three different hydrophobic coating materials by the top-spray method. The experiments were conducted with polymethacrylate coating material formulated as a water dispersion, and ethyl-ce...

Dogot, T.; Xanthoulis, Y.; Fonder, N.; Xanthoulis, D., 2010: Estimating the costs of collective treatment of wastewater: the case of Walloon Region (Belgium). This paper contributes to a better understanding of costs for collective wastewater treatment in the Walloon Region (Belgium). Based on a large set of data, unit costs to population equivalents are modelled. Considering investment as well as explo...

Warshaw P.R.; Davis F.D., 1985: Disentangling behavioral intention and behavioral expectation. Social psychologists have extensively researched behavioral intention and its relation to future behavior, usually within the framework of M. Fishbein and I. Ajzen's theory of reasoned action. However, the field has confounded 2 separate cons...