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Transcription activation by cAMP receptor protein (CRP) at the Escherichia coli gal P1 promoter: Crucial role for the spacing between the CRP binding site and the minus 10 region


, : Transcription activation by cAMP receptor protein (CRP) at the Escherichia coli gal P1 promoter: Crucial role for the spacing between the CRP binding site and the minus 10 region. Biochemistry 31(40): 9647-9656

The cAMP-CRP complex activates the initiation of transcription at the Escherichia coli gal P1 promoter, and the activation efficiency is highly sensitive to the location of the complex on this promoter region. Moving the CRP binding site by one base pair toward the start of transcription significantly decreases the extent of activation in vivo and actually turns the cAMP-CRP complex into an inhibitor in in vitro experiments.

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