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Transcriptional repression of gdhA in Escherichia coli is mediated by the Nac protein

, : Transcriptional repression of gdhA in Escherichia coli is mediated by the Nac protein. FEMS Microbiology Letters 167(1): 51-56, Oct 1

In this work we show that the nac gene from Escherichia coli is transcriptionally active, and that its expression is dependent on NRI (NtrC) and sigma-54. Northern blot experiments show a monocistronic nac-specific mRNA that is detected when wild-type cells are grown in nitrogen-limiting conditions. Our data also show that in nitrogen-limiting conditions Nac is involved in the transcriptional repression of the gdhA gene (encoding glutamate dehydrogenase) except when L-glutamine is used as the only nitrogen source. Moreover, the high level of GDH activity observed in a nac mutant strain is reduced when a wild-type nac gene is introduced under control of the lac promoter in N-limiting conditions, but not in L-glutamine or N-excess. These results suggest the existence of an additional mechanism responsible for overcoming repression by Nac.


PMID: 9785451

DOI: 10.1111/j.1574-6968.1998.tb13206.x

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