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Transcriptional repression of gdhA in Escherichia coli is mediated by the Nac protein


, : Transcriptional repression of gdhA in Escherichia coli is mediated by the Nac protein. FEMS Microbiology Letters 167(1): 51-56, Oct 1

In this work we show that the nac gene from Escherichia coli is transcriptionally active, and that its expression is dependent on NRI (NtrC) and sigma-54. Northern blot experiments show a monocistronic nac-specific mRNA that is detected when wild-type cells are grown in nitrogen-limiting conditions. Our data also show that in nitrogen-limiting conditions Nac is involved in the transcriptional repression of the gdhA gene (encoding glutamate dehydrogenase) except when L-glutamine is used as the only nitrogen source. Moreover, the high level of GDH activity observed in a nac mutant strain is reduced when a wild-type nac gene is introduced under control of the lac promoter in N-limiting conditions, but not in L-glutamine or N-excess. These results suggest the existence of an additional mechanism responsible for overcoming repression by Nac.

US$19.90

PMID: 9785451

DOI: 10.1111/j.1574-6968.1998.tb13206.x


Other references

Boyer W.N.; Cross H.A., 1972: Effect of reward sequence on extinction and spontaneous recovery. Perceptual & Motor Skills 35(2): 668-670

Marinescu, V.; Loomis, P.A.; Ehmann, S.; Beales, M.; Potashkin, J.A., 2007: Regulation of retention of FosB intron 4 by PTB. One effect of stressors such as chronic drug administration is that sequence within the terminal exon of the transcription factor FosB is recognized as intronic and removed by alternative splicing. This results in an open-reading-frame shift that...

Shin, H.; Shin, D.; Shah, T.; Ro, J., 1993: Optimization of proliferating cell nuclear antigen immunohistochemical staining by microwave oven heating of formalin-fixed, paraffin-embedded tissue. Laboratory Investigation 68(1): 140A

deCarvalho, T.N.; Subedi, A.; Rock, J.; Harfe, B.D.; Thisse, C.; Thisse, B.; Halpern, M.E.; Hong, E., 2015: Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish. The role of the habenular nuclei in modulating fear and reward pathways has sparked a renewed interest in this conserved forebrain region. The bilaterally paired habenular nuclei, each consisting of a medial/dorsal and lateral/ventral nucleus, can...

Waters-Rist, A.L.; Bazaliiskii, V.I.; Weber, A.W.; Katzenberg, M.Anne., 2011: Infant and child diet in Neolithic hunter-fisher-gatherers from Cis-Baikal, Siberia: intra-long bone stable nitrogen and carbon isotope ratios. Analysis of stable nitrogen and carbon isotopes (δ(15) N and δ(13) C) from subadults and adults allows for assessment of age-related dietary changes, including breastfeeding and weaning, and adoption of an adult diet. In one of the first studies...

Gehlert, S.; Chang, C.H.; Hartlage, S., 1997: Establishing the diagnostic validity of premenstrual dysphoric disorder using rasch analysis. Premenstrual Dysphoric Disorder (PMDD) has remained in appendices of the last two editions of The Diagnostic and Statistical Manual of Mental Disorders due to lack of empirical study. Items included in its set of research criteria are considered t...

McMillan, A.; Pakianathan, M.; Mao, J.H.; Macintyre, C.C., 1994: Urethral stricture and urethritis in men in Scotland. Objectives: To examine the incidence of urethral stricture in men in Scotland during the years 1982-1991 in relation to the changing incidence of gonococcal and non-gonococcal urethritis (NGU) over the past 20 years. Design: Retrospective study of...

T.R.D.vies; D.F.T.erien, 1971: Population description of a denitrifying microbial system. Water Research 5(8): 553-564

Pellegrin, J., 1924: Le Synodontis courteti Pellegrin, Poisson du Chari et du Niger. Bulletin du Museum Paris, 355-356

Bernstein-Goral, H.; Bregman, B.S., 1993: Spinal cord transplants support the regeneration of axotomized neurons after spinal cord lesions at birth: a quantitative double-labeling study. After spinal cord lesions in newborn rats, transplants of fetal spinal cord tissue rescue immature axotomized neurons, support the growth of axons into and through the site of injury and prolong the critical period for developmental plasticity. Bo...