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Two ineffective-nodulating mutants of Lotus japonicus-different phenotypes caused by the blockage of endocytotic bacterial release and nodule maturation


, : Two ineffective-nodulating mutants of Lotus japonicus-different phenotypes caused by the blockage of endocytotic bacterial release and nodule maturation. Plant & Cell Physiology 38(7): 871-881

Mutants defective in nodule development and nitrogen fixation of Lotus japonicus B-129 'Gifu' were obtained by induced mutagenesis with EMS (ethylmethane sulfonate) treatment. Using a symbiont of L. japonicus, Rhizobium loti JRL501, 17,000 M2 seeds were screened for plants affected in their symbiotic phenotype, resulting in the successful isolation of eleven stable mutants. In this paper, we report two ineffective nodulating mutants among them. Reciprocal crossing between wild type 'Gifu' and these mutants indicated that their phenotypes are under monogenic and recessive control. Furthermore, tests for allelism with these mutants showed that the mutated genes are non-allelic. Ultrastructural analysis revealed that these mutants were inhibited at different stages of nodule development and maturation. Based on histological characteristics of the nodules, two ineffective nodulating mutants were named alb1 (aberrant localization of bacteria inside nodule) and fen1 (fail in enlargement of infected cells), respectively. In the nodules of alb1, most of the bacteria failed to invade the cytoplasm of cortical cells and were tightly confined inside infection threads or localized in intercellular spaces of nodules. Following prolonged inoculation, alb1 mutant also formed pale-pink colored nodules with a low frequency, in which bacteria differentiated into bacteroid and fixed nitrogen normally. Although the infected cells in the nodules of fen1 mutant contained numerous differentiated bacteroids, they failed to enlarge by cell expansion and showed a low activity of nitrogen fixation.

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DOI: 10.1093/oxfordjournals.pcp.a029246


Other references

Bolt, A.G.; Forrest, I.S., 1967: Assay of chlorpromazine glucuronides in human urine. Journal of Pharmaceutical Sciences 56(11): 1533-1534

Nakai, T.; Yasuhara, T.; Fujiki, Y.; Ohashi, A., 1995: Multiple genes, including a member of the AAA family, are essential for degradation of unassembled subunit 2 of cytochrome c oxidase in yeast mitochondria. Cytochrome c oxidase consists of three mitochondrion- and several nucleus-encoded subunits. We previously found that in a mutant of Saccharomyces cerevisiae lacking nucleus-encoded subunit 4 of this enzyme (coxIV), subunits 2 and 3 (CoxII and CoxI...

Klein, B.D.; Barton, M.E.; Wolf, H.H.; White, H.S.eve, 2001: Pharmacologic effects of antiepileptic drug treatment on c-Fos immunoreactivity in the 6-Hz psychomotor seizure model of partial epilepsy. Epilepsia 42(Supplement 7): 127

Clark W.A.Jr; Everett A.W.; Fitch F.W.; Frogner K.S.; Jakovcic S.; Rabinowitz M.; Warner A.M.; Zak R., 1980: Characterization of mono clonal antibodies directed against determinants on cardiac myosin heavy chain. Monoclonal antibodies against cardiac myosin from chicken (18 day embryos) and rabbit were produced in vitro by fusion of mouse myeloma cells (P3-X63-Ag8) with spleen cells from immunized BALB/c mice and Lewis rats. Three antibodies reacted with d...

Gonzalez, N.S.; Algranati, I.D., 1994: Regulation of putrescine uptake in Leishmania mexicana promastigotes. Putrescine uptake of Leishmania mexicana promastigotes is tightly regulated by polyamine intracellular concentrations. This uptake, markedly stimulated after parasite treatment with alpha-difluoromethylornithine (DFMO) for 48 to 72 hrs., was stron...

Bowling, A.; Windsor, J., 1998: Discriminative power of the health status questionnaire 12 in relation to age, sex, and longstanding illness: findings from a survey of households in Great Britain. To assess the ability of the health status questionnaire 12 (HSQ-12) to discriminate between older and younger age groups, its appropriateness for use with an older population in terms of the spread of responses across categories, floor or ceiling...

Solovov, V.P., 1970: The biology of the Bream Abramis brama (L.) in the Upper Obj. Vop. Ikhtiol.: 10: 790-796,In Russian

Kos ko M.K.; Glebovskiy V.Y.; Korago E.A.; Maschenkov S.P.; Piskarev A.L.; Shipelkevich Y.V.; Suprunenko O.I.; Verba V.V.; Zayonchek A.V., 1998: Tectonic basement of the Eurasian Arctic Shelf; structural and compositional features. Pages 103 1998

Sherwood, D.; Hosmer, H.G., 1984: Harriet Hosmer's sojourn in St. Louis. Gateway Heritage 5(3): 42-48

Vyrenkova, N.Iu.; Faradzheva, N.A., 1989: The mucociliary transport function of the lungs in chronic bronchitis and the radionuclide methods for its study (a review). Meditsinskaia Radiologiia 34(11): 16-22