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Typing Neisseria meningitidis by analysis of restriction fragment length polymorphisms in the gene encoding the class 1 outer membrane protein: application to assessment of epidemics throughout the last 4 decades in China


, : Typing Neisseria meningitidis by analysis of restriction fragment length polymorphisms in the gene encoding the class 1 outer membrane protein: application to assessment of epidemics throughout the last 4 decades in China. Journal of Clinical Microbiology 33(2): 458-462

A typing method was developed for Neisseria meningitidis serogroup A by analysis of restriction fragment length polymorphisms (RFLP) of the class 1 outer membrane protein gene (porA). By using appropriate primers, an approximately 1,116-bp fragment of the porA gene was amplified by PCR and then was digested with the restriction endonuclease MspI. The digestion products were separated on 10% polyacrylamide gels and were stained with silver. One hundred three clinical isolates of group A N. meningitidis from 17 provinces of China collected over a 26-year period were analyzed. Results of MspI-generated RFLP profiles of PCR-amplified porA genes were compared with those obtained by conventional serosubtyping. There was a band of about 400 bp common to all strains examined, and the 103 strains of serogroup A resulted in 22 unique RFLP patterns. The differences in bands could be observed mainly in the range of 120 to 280 bp. The smaller fragments were useful in distinguishing meningococci with the same serosubtype. Three epidemic periods were characterized by the presence of three distinct genotypes (a1, a2, and a3), accounting for 74.5% of the strains examined (3.88, 26.21, and 44.66%, respectively). Three predominant RFLP patterns were correlated epidemiologically with cycles of epidemic meningococcal meningitis and were well-matched to the predominant serosubtypes (P1.9, P1.7, 10, and P1.9) that presented at the same prevalence cycles. The genotyping yielded information that allowed strains from one epidemic to be distinguished from those from another that would have been indistinguishable if only serotyping and serosubtyping were available. Therefore, the PCR-RFLP typing method was very useful in the epidemiologic investigation of group A meningococcal meningitis.

US$29.90

PMID: 7714207


Other references

Kisza, J.; Ozimek, L., 1981: Studies on humanization of the fat fraction of dried milk and infant foods. III. Changes in the fat fraction of dried milk in relation to the method of humanization. Milk with increased content of unsaturated fatty acids and tocopherols produced by cow feeding as described in parts I and II [DSA 43, 4737, 4738] or by addition of soyabean oil (7.4:2.6 ratio of milk fat to oil) was standardized to 3.1-3.15% fat,...

Hoyme, U.; Baumueller, A.; Madsen, P.O., 1977: Rosamicin in urethral and vaginal secretions and tissues in dogs and rats. In animal studies we investigated the distribution of rosamicin in plasma and urethral and vaginal tissues in rats as well as in urethral and vaginal secretions in dogs. We found concentration ratios between urethral secretion and plasma of 1.9 an...

Hachiya, T.; Minei, S.; Kobayashi, K.; Ishida, H.; Okada, K., 2001: Prediction of organ-confined disease by prostate-specific antigen nadir after neoadjuvant therapy. It is not clear whether or not serum prostate-specific antigen (PSA) levels after androgen deprivation prior to radical prostatectomy (neoadjuvant therapy) have any value in the prediction of the final pathologic stage. We conducted a study on 49...

Anderson, A.B.; E.A., 1968: Composition of Mindoro Pine gum oleoresin. Pinus merkusii, now scarce in the Philippines, is being tried for afforestation. It yields a good gum oleoresin containing 13% turpentine (97% alpha-pinene, 1% n-undecane, 1% alpha-phellandrene, with traces of beta-pinene and two other terpenes)....

Harmsen, H.; Neumann, R.O., 1952: R. O. Neumann. Archiv für Hygiene und Bakteriologie 136(4): 258-260

Romand, Dée.; Baclet, N., 2006: A diet education adapted to the cultural habits of diabetic patients. Soins; la Revue de Reference Infirmiere: 23-25

Moens, R., 1963: Studies on the subterranean slug Milax buda-pestensis Hazay.. The life cycle of this slug is described; it can do considerable damage to potatoes, carrots and various ornamental plants. Metaldehyde baits (4-5%) are attractive to the slugs; ground sprays of DNOC applied in the autumn to ground which has no pl...

Maxwell, E.E.; Caldwell, M.W.; Lamoureux, D.O.; Budney, L.A., 2012: Histology of tooth attachment tissues and plicidentine in Varanus (Reptilia: Squamata), and a discussion of the evolution of amniote tooth attachment. Few recent studies have examined the histological basis for tooth attachment in squamates. In the past few years, a surge of interest in this topic has led to the intriguing suggestion that the major tissues derived from the tooth germ (enamel, de...

Shelef, G., 1989: The engineering of microalgae mass cultures for treatment of agricultural wastewater, with special emphasis on selenium removal from drainage waters. Biotreatment of agricultural wastewater editor Mark E Huntley: 148

Phogat, D.C.; Chawla, V.; Luthra, O.P.; Uppal, S., 1995: Genetic architecture of area under disease progress curve and its association with slow leaf rusting and yield traits in wheat. Six wheat genetic stocks and 15 F-1's constituted the material to obtain the informations on combining abilities for AUDPC and its association with slow leaf rusting components and yield traits. Combining ability analysis indicated that both...