geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Tyrosine phosphorylation and bradykinin-induced signaling in endothelial cells


, : Tyrosine phosphorylation and bradykinin-induced signaling in endothelial cells. American Journal of Cardiology 80(SUPPL 3A): 102A-109A

It is generally accepted that in endothelial cells the occupation of bradykinin B-2 receptors, which are linked to the guanine nucleotide-dependent regulatory proteins, G-i and G-q, results in the activation of phospholipase C-beta-i (PLC-beta-1), followed by a transient increase in the formation of inositol 1,4,5-trisphosphate (IP-3) and diacylglycerol. The PLC-beta-1 isoform, in contrast to the gamma-1 isoform, is present only at a low level in cultured endothelial cells, implying that PLC-gamma-1 activation may play an important role in endothelial signaling pathways. In cultured human endothelial cells, bradykinin induced a rapid increase in the tyrosine phosphorylation of several Triton-soluble proteins. Immunoprecipitation of tyrosine-phosphorylated proteins from bradykinin-stimulated cells followed by Western blotting using the respective antibodies facilitated the identification of a 77 kiloDalton (kDa) protein as paxillin, a 130 kDa protein as PLC-gamma-1, and a 42/44 kDa doublet as mitogen-activated protein (MAP) kinase. The bradykinin-induced tyrosine phosphorylation of PLC-gamma-1 was relatively transient and was associated with an increase in intracellular levels of IP-3. Bradykinin also induced the rapid and transient activation of phosphotyrosine phosphatases localized mainly in the Triton X-100-soluble cell fraction; this tyrosine phosphatase activity was apparently initiated after the release of Ca-2+ from intracellular stores.

US$19.90

PMID: 9293962

DOI: 10.1016/s0002-9149(97)00464-5


Other references

Rice, B., 2006: Watch out for this malpractice trap!. Medical Economics 83(9): 42-45

Garrido L.; Gainza J.; Pereira E., 1988: Influence of sodium silicate on the rheological behaviour of clay suspensions; application of the ternary Bingham model. Applied Clay Science 3(4): 323-335

Anonymous, 1968: Cethexonium bromide. Annales Pharmaceutiques Francaises 26(12): 805-806

Kodera, Y.; Akiyama, S.; Isobe, K.; Kondo, K.; Ito, K.; Yamauchi, M.; Takagi, H., 1994: Expression of pi-Glutathione S-transferase Gene in Gastric Cancer Lack of Correlation With Resistance Against Cis-diamminedichloroplatinum. Class pi-glutathione S-transferase (GSTP-1) is one of several factors proposed to affect drug sensitivity to cisdiamminedichloroplatinum (II) (CDDP). It has also been investigated as a potential marker for the serodiagnosis of various types of can...

von Hoyningen Huene Wolfgang; Wenzel Karin, 1997: Radiative properties of soil-derived dust and its effect on radiative balance. Terra Nostra (Bonn) 97-10: 64-83

Voyer, M.; Dehan, M., 1985: What is the prognosis for premature infants? Outcome until school age (2). Archives Francaises de Pediatrie 42(10): 879-887

Abivardi, C., 1978: Status of root-knot nematodes, Meloidogyne spp., in Iran. The host range and regional distribution of Meloidogyne spp. in Iran are given.

Biglioli, P., 1965: Treatment Of Sprains By Means Of Periarticular And Intraligamentous Infiltrations. Giornale di Medicina Militare 115: 71-77

Murugan, K.; Dinesh, D.; Kumar, P.Jenil.; Panneerselvam, C.; Subramaniam, J.; Madhiyazhagan, P.; Suresh, U.; Nicoletti, M.; Alarfaj, A.A.; Munusamy, M.A.; Higuchi, A.; Mehlhorn, H.; Benelli, G., 2016: Datura metel-synthesized silver nanoparticles magnify predation of dragonfly nymphs against the malaria vector Anopheles stephensi. Malaria is a life-threatening disease caused by parasites transmitted to people and animals through the bites of infected mosquitoes. The employ of synthetic insecticides to control Anopheles populations leads to high operational costs, non-target...

Zoulek, G.; Roggendorf, M.; Deinhardt, F.; Kunz, C., 1986: Different immune responses after intradermal and intramuscular administration of vaccine against tick-borne encephalitis virus. A single multisite intradermal (ID) administration of the same dose used for regular intramuscular (IM) immunisation with vaccine against tick-borne encephalitis virus (TBEV) resulted in seroconversion of all vaccinees within three weeks: with the...