+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ultrastructure of the frog retina after high-pressure freezing and freeze substitution

, : Ultrastructure of the frog retina after high-pressure freezing and freeze substitution. Journal of Microscopy (Oxford) 189(3): 219-235, March

In many types of tissue, high-pressure freezing (HPF), followed by freeze substitution, can produce excellent ultrastructural preservation at depths over 10 times that obtained by other cryofixation techniques. However, in the case of neural tissue, the benefits of HPF have not been realized. In the present study, isolated frog (Rana pipiens) retina was sliced at a thickness of 150 or 350 mum, rapidly frozen in a Balzers HIM 010 high-pressure freezer, and 15% low-MW dextran for 350-mum slices) were required for freezing media (35% high-MW dextran for 150-mum slices or adequate freezing. The quality of preservation after HPF was excellent throughout the retina in both the 150- and 350-mum slices, compared with chemically fixed slices. Specifically, HPF resulted in better preserved cellular, mitochondrial and nuclear membranes in all retinal layers. This is the first study to successfully cryofix all of the layers of the retina. The increased depths of adequate freezing achieved by HPF should facilitate various ultrastructural studies of retina, as well as of other CNS tissues, where preservation approaching that of the 'native' state is required.


PMID: 9588021

DOI: 10.1046/j.1365-2818.1998.00315.x

Other references

Kohn, B., 1994: Zoo animal welfare. The history of zoo animal welfare legislation extends back to 1876, and is often tied to general animal welfare regulations. As knowledge and societal values have changed, so have the focus of zoos and the regulations governing them. Today, the is...

Hamilton, D.R., 1990: How can i avoid lyme disease? What to say to the patient who asks. Measures that can be taken by individuals to decrease the risk of contracting Lyme disease (caused by Borrelia burgdorferi) by minimizing personal exposure to the tick vectors, maximizing personal protection against ticks and reducing numbers of t...

Chavanet, P.; Beloeil, H.; Pechinot, A.; Duigou, F.; Buisson, J.C.; Duong, M.; Neuwirth, C.; Kazmierczak, A.; Portier, H., 1995: In vivo activity and pharmacodynamics of cefotaxime or ceftriaxone in combination with fosfomycin in fibrin clots infected with highly penicillin-resistant Streptococcus pneumoniae. Using a clinical pneumococcal strain for which MICs were 2, 0.5, 0.5, and 16 mg/liter for penicillin, cefotaxime, ceftriaxone, and fosfomycin, respectively, we studied the efficacies of these antibiotics alone and in combination in one or two dose...

Penumetcha, M.; Santanam, N., 2012: Nutraceuticals as Ligands of PPARγ. Peroxisome proliferator-activated receptors (PPARs) are ligand-activated nuclear receptors that respond to several exogenous and endogenous ligands by modulating genes related to lipid, glucose, and insulin homeostasis. PPARγ, expressed in adipos...

Lane, S.H.; Kohlenberg, E., 2011: The future of baccalaureate degrees for nurses. PROBLEM.  Unlike other professional healthcare disciplines, the profession of nursing has multiple levels of entry. Recently, several states have proposed legislation to mandate completion of baccalaureate education after 10 years of nursing lic...

Decelle, J., 1969: Contribution a la faune du Congo (Brazzaville). Mission A. Villiers et A. Descarpentries 88. Coleopteres, Melolonthidae, Hopliinae. Bull Inst fond Afr noire (Ser A): 31: 1042

Prefumo, F.; Frusca, T., 2014: Comment on "long-term outcomes of children with umbilical vein varix diagnosed prenatally". Prenatal Diagnosis 33(11): 1113-1113

Patil P.S.; Elem B.; Kasolo F., 1992: Impact of human immunodeficiency viral infection upon malignancies among indigenous zambians. Viii International Conference on Aids And The Iii Std World Congress Published Abstracts Submitted to The Viii International Conference on Aids And The Iii Std World Congress; Harvard-Amsterdam Conference, Amsterdam, Netherlands, July 19-24, 220p Viii International Congress And The Iii Std World Congress: Amsterdam, Netherlands Paper : 117

Blades, S.; Bhatia, A.; Kielty, C.; Edwards, J., 1998: Immunoregulatory molecules bound to microfibrils A clue to the link between polymyalgia and rheumatoid arthritis. British Journal of Rheumatology 37(ABSTR SUPPL 1): 35

Lund R.D., 1987: Functional impact of retinal transplants. Neuroscienceppl: S262