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Ultrastructure, pharmacologic inhibition, and transport selectivity of aquaporin channel-forming integral protein in proteoliposomes


, : Ultrastructure, pharmacologic inhibition, and transport selectivity of aquaporin channel-forming integral protein in proteoliposomes. Biochemistry 33(6): 1606-1615

Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (P-f) to the membranes (Zeideletal. (1992) Biochemistry 31, 7436-7440). Here were port detailed ultrastructural, pharmacologic, and transport studies of human red cell CHIP in proteoliposomes. Freeze-fracture and transmission electron microscopy revealed a uniform distribution of CHIP which was incorporated into the membranes in both native and inverse orientations. Morphometric analysis of membranes reconstituted at three different concentrations of CHIP revealed that the intramembrane particles correspond to tetramers or possible higher order oligomers, and the P-f increased in direct proportion to the CHIP density. Proteolytic removal of the 4-kDa C-terminal cytoplasmic domain of CHIP did not alter the P-f or oligomerization in red cell membranes. CHIP exhibited a similar conductance for water when reconstituted into membranes of varied lipid compositions. The sensitivities of CHIP-mediated P-f to specific sulfhydryl reagents were identical to known sensitivities of red cell P-f, including a delayed response top-(chloromercuri)benzenesulfonate. CHIP did not increase the permeability of the proteoliposome membranes to H+/OH- or NH-3. These studies demonstrate that CHIP proteoliposomes exhibit all known characteristics of water channels in native red cells and therefore provide a defined system for biophysical analysis of transmembrane water movements.

US$29.90

PMID: 8312280


Other references

Porcellati, G.; D.J.so, F.; Malcovati, M.; Biasion, M.G., 1966: The conversion of the ethanolamine phosphate moiety of L-serine ethanolamine phosphate to microsomal phospholipid in brain tissue. Life Sciences 5(19): 1791-1799

Lakshmanan S., 1973: Evolution of the Son drainage. Proceedings of the Indian National Science Academy, Part A: Physical Sciences 38(1-2): 21-31

Larrègue, M.; Bressieux, J.M.; Villatte, D.; Champion, R.; Ramdenée, P.; Jacquemin, J.L.; Prigent, F., 1981: Native favus in Poitou. About 3 cases. Three cases of tinea favus are reported in children from a small town in the Poitou region. Trichophyton schoenleinii was cultured from 2 of the cases.

Fugazza, J., 2014: Fluorimetric and biological determination of cerebral noradrenalin. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 155: 284-285

Gawad, K.A.; Knoefel, W.T.; Izbicki, J.R.; Schröder, S.; Broelsch, C.E., 1996: Generalised lymphangioleiomyomatosis presenting as a retroperitoneal tumour. European Journal of Surgery 162(7): 583-585

Clausen, J.; Kjaergaard, J.; Bierring, F., 1981: The ultrastructure of the dermo-epidermal junction in lichen planus. The ultrastructure of the dermo-epidermal junctional area in biopsy material from 5 patients suffering from lichen planus has been studied during treatment with steroids. Tannic acid was used on the tissue blocks in order to provide high and diver...

Nagata, T.; Fukuoka, H., 1968: Control of the brown planthopper with diazinon granules. The following is substantially the English abstract. In a test in Kyushu, Japan, diazinon granules were applied to rice plants in the field in August for the control of Nilaparvata lugens (Stal). A 3% granular formulation at the rate of 3 kg per 1...

Braithwaite J.C., 1980: Dolomite production by Lime and Marble Limited, Mt. Burnett, New Zealand. Monograph Series - Australasian Institute of Mining and Metallurgy 10): 690-691

Williamson, M.-Claude; Smyth, H.R.; Peterson, R.C.; Lavoie, D., 2011: Comparative geological studies of volcanic terrain on Mars; examples from the Isachsen Formation, Axel Heiberg Island, Canadian High Arctic. Recent orbital and rover missions to Mars have returned high-resolution images that show complex surface landforms in unprecedented detail. In addition, the spectral data sets from mission instruments reveal the presence of a wide array of mineral...

Arystarkhova, E.; Bouley, R.; Liu, Y.Bessie.; Sweadner, K.J., 2017: Impaired AQP2 trafficking in Fxyd1 knockout mice: A role for FXYD1 in regulated vesicular transport. The final adjustment of urine volume occurs in the inner medullary collecting duct (IMCD), chiefly mediated by the water channel aquaporin 2 (AQP2). With vasopressin stimulation, AQP2 accumulation in the apical plasma membrane of principal cells a...