+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Use of pulsed-field gel electrophoresis to determine genomic diversity in strains of Helicobacter hepaticus from geographically distant locations

, : Use of pulsed-field gel electrophoresis to determine genomic diversity in strains of Helicobacter hepaticus from geographically distant locations. Journal of Clinical Microbiology 35(11): 2859-2863

In 1992 a helical microorganism associated with chronic active hepatitis and a high incidence of hepatocellular tumors was identified in the hepatic parenchyma of A/JCr mice. By using biochemical tests, phenotypic characterization, and 16S rRNA gene sequence analysis, the organism was classified as a novel Helicobacter species and named Helicobacter hepaticus. Recent surveys completed in our laboratory indicate that H. hepaticus is widespread in academic and commercial mouse colonies. The aim of this study was to examine the H. hepaticus genome by pulsed-field gel electrophoresis (PFGE) to determine the degree of genomic variation and genomic size. This technique has been used to identify significant genomic diversity among strains of Helicobacter pylori and to demonstrate only slight genomic diversity among strains of Helicobacter mustelae. Genomic DNAs from 11 isolates of H. hepaticus from the United States, Germany, France, and The Netherlands were subjected to PFGE after digestion with SmaI. Isolates from three independent sources within the United States had very similar PFGE patterns, suggesting that the genomic DNAs of these isolates are conserved. Genomic DNA isolated from a fourth source within the United States had a PFGE pattern different from those of the other U.S. isolates. Isolates obtained from Germany, France, and The Netherlands had PFGE patterns that differed markedly from those of the U.S. isolates and from one another. The use of DNA fingerprinting may be useful in subsequent epidemiological studies of H. hepaticus when the source and method of spread of this murine pathogen need to be ascertained. By PFGE, the genomic size of H. hepaticus is estimated to be roughly 1.3 Mb, which compares to 1.67 Mb for H. pylori and 1.7 Mb for H. mustelae.


PMID: 9350747

Other references

Brandon, C.A.; Rosen, C.; Georgelis, G.; Horton, M.J.; Mooney, M.P.; Sciote, J.J., 2003: Staining of human thyroarytenoid muscle with myosin antibodies reveals some unique extrafusal fibers, but no muscle spindles. This study describes the myosin composition of extrafusal and intrafusal muscle fibers found in the human thyroarytenoid (TA) and sternohyoid (control) muscles. We sought to determine the presence of muscle spindles in the TA muscle, and to identi...

Pipik, R.; Holec, P., 1998: Pannonian ostracods Crustacea, Ostracoda and vertebrates Chordata, Vertebrata from loam pit of the brick yard in Borsky Svaty Jur Panonske lasturnicky Crustacea, Ostracoda a stavovce Chordata, Vertebrata z hliniska tehelne v Borskom Svatom Jure. Mineralia Slovaca, 303: 185-194

Ferreira da Silva, A.R., 2007: A Dirichlet process mixture model for brain MRI tissue classification. Accurate classification of magnetic resonance images according to tissue type or region of interest has become a critical requirement in diagnosis, treatment planning, and cognitive neuroscience. Several authors have shown that finite mixture mode...

Cunningham, M.A.; Darby, D.G.; Donnan, G.A., 1991: Controlled-release delivery of L-dopa associated with nonfatal hyperthermia, rigidity, and autonomic dysfunction. Neurology 41(6): 942-943

Yang, Z.; Hu, M.; Wang, X., 2007: Poly[μ(3)-aqua-μ(2)-2,4-dinitro-phenolato-rubidium(I). The asymmetric unit of the title compound, [Rb(C(6)H(3)N(2)O(5))(H(2)O)](n), comprises a rubidium cation, a 2,4-dinitro-phenoxide anion and a water mol-ecule. The Rb(+) cation is 11-coordinated by O atoms from 2,4-dinitro-phenolate anions and wate...

Pericak-Vance, M., A.; Bass, M.L.; Yamaoka, L.H.; Gaskell, P.C.; Scott, W.K.; Terwedow, H.A.; Menold, M.M.; Conneally, P.M.; Small, G.W.; Saunders, A.M.; Roses, A.D.; Haines, J.L., 1998: Complete genomic screen in late-onset familial Alzheimer's disease. Alzheimer's disease (AD) is a complex genetic disorder. Linkage analysis has helped unravel a portion of the genetic component of AD by identifying four loci that play a role in the genetics of AD (amyloid precursor protein, presenilin 1, pre...

Salm, P.; Taylor, P.J.; Roberts, D.; de Silva, J., 2009: Liquid chromatography-tandem mass spectrometry method for the simultaneous quantitative determination of the organophosphorus pesticides dimethoate, fenthion, diazinon and chlorpyrifos in human blood. Simultaneous determination of the organophosphorus pesticides dimethoate, fenthion, diazinon and chlorpyrifos in human blood by HPLC-tandem mass spectrometry was developed and validated. The pesticides were extracted by a simple one-step protein p...

Hirono T.; Nakashima S.; Spiers C.J., 2003: Reduction of ionic diffusivity in nanopore water of geomaterials. Eos, Transactions, American Geophysical Union 84(46, Suppl

Chen, L.; Ueta, H.; Bisson, Régis.; Beck, R.D., 2014: Quantum state-resolved gas/surface reaction dynamics probed by reflection absorption infrared spectroscopy. We report the design and characterization of a new molecular-beam/surface-science apparatus for quantum state-resolved studies of gas/surface reaction dynamics combining optical state-specific reactant preparation in a molecular beam by rapid adia...

Jordan, S.W.; Corcoran, J., 2013: Considerations in breast augmentation in the adolescent patient. Nearly 320,000 breast augmentations were performed in 2011, with adolescents under 18 years of age accounting for 4,830 procedures (1.5%). Breast development typically starts at 11 years (range, 8-13 years) and is complete at 15 years (range, 11-1...