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DNA packing in stable lipid complexes designed for gene transfer imitates DNA compaction in bacteriophage


, : DNA packing in stable lipid complexes designed for gene transfer imitates DNA compaction in bacteriophage. Proceedings of the National Academy of Sciences of the United States of America 96(22): 293-8

The structure of complexes made from DNA and suitable lipids (lipoplex, Lx) was examined by cryo-electron microscopy (cryoEM). We observed a distinct concentric ring-like pattern with striated shells when using plasmid DNA. These spherical multilamellar particles have a mean diameter of 254 nm with repetitive spacing of 7.5 nm with striation of 5.3 nm width. Small angle x-ray scattering revealed repetitive ordering of 6.9 nm, suggesting a lamellar structure containing at least 12 layers. This concentric and lamellar structure with different packing regimes also was observed by cryoEM when using linear double-stranded DNA, single-stranded DNA, and oligodeoxynucleotides. DNA chains could be visualized in DNA/lipid complexes. Such specific supramolecular organization is the result of thermodynamic forces, which cause compaction to occur through concentric winding of DNA in a liquid crystalline phase. CryoEM examination of T4 phage DNA packed either in T4 capsides or in lipidic particles showed similar patterns. Small angle x-ray scattering suggested an hexagonal phase in Lx-T4 DNA. Our results indicate that both lamellar and hexagonal phases may coexist in the same Lx preparation or particle and that transition between both phases may depend on equilibrium influenced by type and length of the DNA used.

US$19.90

PMID: 10535915

DOI: 10.2307/49340


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Loynd, A., 1996: Some notes on a voyage from Hiroshima to Hong Kong. Hong Kong Bird Report. 1995( ); 1995: 250

Pana, A.; Santi, A.L.; Castello, C.; Marchione, L.; D.A.ca, S.U., 1976: Presence of Cytomegalovirus in human milk. Cytomegalovirus was isolated in 4 out of 17 milk samples taken from women within 48 h after delivery, but examination of urine samples for the virus was negative. It is suggested that milk may be an important source of neonatal infection with Cyto...

Timofeeva, E.E., 1969: Anatomopathologic changes in the human intestine caused by Hymenolepis nana. Meditsinskaia Parazitologiia i Parazitarnye Bolezni 38(2): 167-169

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Christiansen O.B.; Andersen E.S.; Lauritsen J.G.; Grunnet N., 1987: Diminished paterno maternal histoincompatibility in habitual abortions. Chaouat, G (Ed ) Colloque Inserm (Institut National De La Sante Et De La Recherche Medicale), Vol 154 Immunologie De La Reproduction: Relation Materno-Foetale: Implications Fondamentales Et Perspectives D'application; (Inserm (National Institute Of Health And Medical Research) Colloquium), Vol 154 Reproductive Immunology: Materno-Fetal Relationship: Fundamental Investigations And Future Applied Prospects; Paris, France, December 3-5, 1986 Xiv+483p Editions Inserm: Paris, France Il

Savi, C., 1957: Diagnostic & therapeutic advances in female genital tuberculosis; statistical report on 194 cases. Rivista D'ostetricia E Ginecologia Pratica 39(10): 933-40 Contd

Harris, P., 1966: Regional metabolism during exercise in heart disease. Bulletin of the New York Academy of Medicine 42(11): 966-981

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