geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Desmoplakin is required for microvascular tube formation in culture


, : Desmoplakin is required for microvascular tube formation in culture. Journal of Cell Science 117(Pt 15): 3129-3140

Desmoplakin (DP) is a key component of cellular adhesion junctions known as desmosomes; however, recent investigations have revealed a novel location for DP in junctions separate from desmosomes termed complexus adherens junctions. These junctions are found at contact sites between endothelial cells that line capillaries. Few studies have focused on the function of DP in de novo capillary formation (vasculogenesis) and branching (angiogenesis) during tumorigenesis, embryonic development, cardiovascular development or wound healing. Only recently have investigations begun to determine the effect the loss of DP has on capillaries during embryogenesis (i.e. in DP-/- mice). Evidence shows that the loss of desmoplakin in vivo results in leaky capillaries and/or capillary malformation. Consequently, the goal of this study was to determine the function of DP in complexus adherens junctions during capillary formation. To accomplish this goal, we used siRNA technology to knock down desmoplakin expression in endothelial cells before they were induced to form microvascular tubes on matrigel. DP siRNA treated cells sent out filopodia and came in close contact with each other when plated onto matrigel; however, in most cases they failed to form tubes as compared with control endothelial cells. Interestingly, after siRNA degradation, endothelial cells were then capable of forming microvascular tubes. In depth analyses into the function of DP in capillary formation were not previously possible because the tools and experimental approaches only recently have become available (i.e. siRNA). Consequently, fully understanding the role of desmoplakin in capillary formation may lead to a novel approach for inhibiting vasculo- and angiogenesis in tumor formation. Reprinted by permission of the publisher.

US$19.90

PMID: 15190119

DOI: 10.1242/jcs.01132


Other references

Allen J.R.L.; Rae J.E.; Zanin P.E., 1990: Metal speciation copper zinc lead and organic matter in an oxic salt marsh severn estuary southwestern britain uk. Predominantly anthropogenic, Cu, Zn, and Pb present in an approximately 100 yr old mud flat and salt marsh deposit at a high intertidal, oxic site are bound chiefly in the Fe-Mb oxide-hydroxide, organic and residual phases. The amount of each meta...

Mozafari, M.R.; Javanmard, R.; Raji, M., 2017: Tocosome: Novel drug delivery system containing phospholipids and tocopheryl phosphates. The potential of two derivatives of vitamin E, i.e. α-tocopheryl phosphate (TP) and di-α-tocopheryl phosphate (T<sub>2</sub>P), for the protection and delivery of therapeutics is being investigated in our laboratory and some promisin...

Theiler, K., 1959: Tail mutants in mice. A contribution to the pathogenesis of spine abnormalities. Zeitschrift für Anatomie und Entwicklungsgeschichte 121: 155-164

Samasca, G.; Deleanu, D.; Sur, G.; Lupan, I.; Giulia, A.; Carpa, R., 2016: Is it necessary to screen iHelicobacter pylori infection in patients with celiac disease and iron deficiency?. Gastroenterology and Hepatology From Bed to Bench 9(4): 345-345

de Oliveira, Márcia.Silva.; Betting, L.Eduardo.; Mory, S.B.; Cendes, F.; Castellano, G., 2013: Texture analysis of magnetic resonance images of patients with juvenile myoclonic epilepsy. Juvenile myoclonic epilepsy (JME) is the most frequent subsyndrome of the idiopathic generalized epilepsies, and experimental investigations support that the thalamus is a key structure in the mechanisms of JME. Texture analysis (TA) is an image p...

Adékambi, Tïdi.; Shinnick, T.M.; Raoult, D.; Drancourt, M., 2008: Complete rpoB gene sequencing as a suitable supplement to DNA-DNA hybridization for bacterial species and genus delineation. DNA-DNA hybridization (DDH), the gold standard for bacterial species delineation, is a laborious method and the alternative, average nucleotide identity (ANI), a genomic sequence-derived parameter, is not applicable to non-sequenced species. A uni...

Anonymous, 1993: The 12th International Immunology and Diabetes Workshop. Proceedings and abstracts. Orlando, Florida, April 1993. Autoimmunity 15 Suppl: 1-89

Raichev, T., 1998: Thermal stability of the humus system. The possibility of thermal analysis to determine the relationship between stability and humus structure through pyrolysis of soil samples was studied. Samples of surface horizons of leached smonitza (Sredetz), leached chernozem (Kochmar), grey for...

Chu, G.M.; Lee, H.J.; Park, J.S.; Cho, H.W.; Ahn, B.H., 2003: Effect of garlic stalk silage on performance and carcass characteristics of Hanwoo steers. This study was conducted to investigate the effect of feeding garlic stalk silage on performance and carcass characteristics of Hanwoo steers (Korea Republic). Results showed that feeding garlic stalk silage as a roughage source to steers during t...

Lue, P.F.; Kaplan, J.G., 1969: The aspartate transcarbamylase and carbamoyl phosphate synthetase of yeast: a multi-functional enzyme complex. Biochemical and Biophysical Research Communications 34(4): 426-433