geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Determination of the substrate-docking site of protein tyrosine kinase C-terminal Src kinase


, : Determination of the substrate-docking site of protein tyrosine kinase C-terminal Src kinase. Proceedings of the National Academy of Sciences of the United States of America 100(25): 14707-14712

Protein tyrosine kinases (PTK) are key enzymes of mammalian signal transduction. For the fidelity of signal transduction, each PTK phosphorylates only one or a few proteins on specific Tyr residues. Substrate specificity is thought to be mediated by PTK-substrate docking interactions and recognition of the phosphorylation site sequence by the kinase active site. However, a substrate-docking site has not been determined on any PTK. C-terminal Src kinase (Csk) is a PTK that specifically phosphorylates Src family kinases on a C-terminal Tyr. In this study, by sequence alignment and site-specific mutagenesis, we located a substrate-docking site on Csk. Mutations in the docking site disabled Csk to phosphorylate, regulate, and complex with Src but only moderately affected its general kinase activity. A peptide mimicking the docking site potently inhibited (IC50 = 21 mM) Csk phosphorylation of Src but only moderately inhibited (IC50 = 422 mM) its general kinase activity. Determination of the substrate-docking site provides the structural basis of substrate specificity in Csk and a model for understanding substrate specificity in other PTKs. Reprinted by permission of the publisher.

US$19.90

PMID: 14657361

DOI: 10.1073/pnas.2534493100


Other references

Zhoulan Yin; Jishu Zeng; Qiyuan Chen, 2009: Effect of oleic acid on gibbsite precipitation from seeded sodium aluminate liquors. Effect of oleic acid on precipitation ratio, agglomeration and morphology of gibbsite was investigated at selected conditions. The results of titrimetric analysis suggest that oleic acid inhibit gibbsite precipitation from seeded sodium aluminate...

Bank, E.R.; Glazer, G.M., 1984: Ultrasonographic detection of infected thrombus in the inferior vena cava. Journal of Ultrasound in Medicine 3(12): 567-568

Vasicek, M., 1947: Remarks on the microbiostratigraphy of the Magur Flyscn in Moravia.. Vestnik Statniho Geologickeho Ustavu Ceskosl, 22: 241-258

Kiefl, C.; Woody, R.W., 2000: Heme distortions and circular dichroism of MD-generated structures of both rotational isomers of sperm whale co-myoglobin. Biophysical Journal 78(1 Part 2): 282A, Jan

Hussain, F.; Spingler, B.; Conrad, F.; Speldrich, M.; Kögerler, P.; Boskovic, C.; Patzke, G.R., 2009: Caesium-templated lanthanoid-containing polyoxotungstates. A family of novel lanthanoid-containing polytungstoarsenate(iii) polyanions with interesting structural features has been isolated: six lacunary {alpha-AsW(9)O(33)} building blocks comprising unusual pyramidal WO(5) units are templated by a caesiu...

Anonymous, 1931: Legal Checks In California On Practice Of Medicine. California and Western Medicine 35(6): 476-477

Schonmeier, H.; Rehbein, G., 1988: Straw manuring in rotations with different cereal percentages on Haplic Phaeozem. In field trials in 1974-81 using rotations including 50, 75 or 100% cereals, incorporating chopped straw 1-3 times in each 4-year rotation or increasing amounts of applied N by 30-60 kg/ha above those usual for the crop of the year had no effect o...

Andersen, P.; Woodworth, J.S., 2015: Tuberculosis vaccines--rethinking the current paradigm. The vaccine discovery paradigm in tuberculosis (TB) has been to mimic the natural immune response to infection. With an emphasis on interferon (IFN)-γ as the main protective cytokine, researchers have selected dominant antigens and administered t...

Lakomy, R.; Sana, J.; Hankeova, S.; Fadrus, P.; Kren, L.; Lzicarova, E.; Svoboda, M.; Dolezelova, H.; Smrcka, M.; Vyzula, R.; Michalek, J.; Hajduch, M.; Slaby, O., 2012: MiR-195, miR-196b, miR-181c, miR-21 expression levels and O-6-methylguanine-DNA methyltransferase methylation status are associated with clinical outcome in glioblastoma patients. Glioblastoma multiforme (GBM) is the most frequently occurring primary malignant brain tumor; patients with GBM often have a very poor prognosis and differing responses to treatment. Therefore, it is very important to find new biomarkers that can...

Martino, A.; Arends, A.; Aranguren, J., 1998: Reproductive pattern of Leptonycteris curasoae Miller (Chiroptera: Phyllostomidae) in northern Venezuala. To determine if there was a latitudinal effect on the reproductive and social pattern of Leptonycteris curasoae, a population from Northern Venezuela (Paraguana, Venezuela) was studied. Monthly sampling took place at Piedra Honda cave, from Novemb...