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Differential MAP kinase activation and Na(+)/H(+) exchanger phosphorylation by H(2)O(2) in rat cardiac myocytes

, : Differential MAP kinase activation and Na(+)/H(+) exchanger phosphorylation by H(2)O(2) in rat cardiac myocytes. American Journal of Physiology. Cell Physiology 281(5): C1542-C1550

Bursts in reactive oxygen species production are important mediators of contractile dysfunction during ischemia-reperfusion injury. Cellular mechanisms that mediate reactive oxygen species-induced changes in cardiac myocyte function have not been fully characterized. In the present study, H2O2 (50 mM) decreased contractility of adult rat ventricular myocytes. H2O2 caused a concentration- and time-dependent activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2), p38, and c-Jun NH2-terminal kinase (JNK) mitogen-activated protein (MAP) kinases in adult rat ventricular myocytes. H2O2 (50 mM) caused transient activation of ERK1/2 and p38 MAP kinase that was detected as early as 5 min, was maximal at 20 min (9.6 [plus or minus] 1.2- and 9.0 [plus or minus] 1.6-fold, respectively, vs. control), and returned to baseline at 60 min. JNK activation occurred more slowly (1.6 [plus or minus] 0.2-fold vs. control at 60 min) but was sustained at 3.5 h. The protein kinase C inhibitor chelerythrine completely blocked JNK activation and reduced ERK1/2 and p38 activation. The tyrosine kinase inhibitors genistein and PP-2 blocked JNK, but not ERK1/2 and p38, activation. H2O2-induced Na+/H+ exchanger phosphorylation was blocked by the MAP kinase kinase inhibitor U-0126 (5 mM). These results demonstrate that H2O2-induced activation of MAP kinases may contribute to cardiac myocyte dysfunction during ischemia-reperfusion. Reprinted by permission of the publisher.


PMID: 11600417

Other references

Schum, A.R.imann-Philipp, R., 1982: Competition phenomena between the three pathotypes of Peronospora spinaciae Laub. as a possible tool for the elimination of pathogenic races by controlled cultivation of host varieties. Zeitschrift fur Pflanzenzuchtung = Journal of plant breeding8(1): 13-24

Woollons, A.; Russell Jones, R., 1998: Chronic endemic hydroarsenicism. Chronic endemic hydroarsenicism in a 48-year-old man from Antofagasta, Chile, is reported. The literature on the global health problems of hydroarsenicism is reviewed, especially with regard to the carcinogenic action of arsenic.

Zhao, J.; Li, S.; Ren, L.; Guo, X.; Qi, X., 2017: Pro-Brain Natriuretic Peptide and Troponin T-Hypersensitivity Levels Correlate With the Severity of Liver Dysfunction in Liver Cirrhosis. Increased pro-brain natriuretic peptide (pro-BNP) or troponin T-hypersensitivity (TnT-HSST) levels are common in liver cirrhosis. We conducted a retrospective observational study aimed to evaluate the correlation of pro-BNP and TnT-HSST levels wit...

Rajaian, H.; Jalaee, J., 2003: Pharmacokinetics of oxytetracycline after intravenous and intramuscular administration in horses. The objective of this study was to determine the pharmacokinetic characteristics of oxytetracycline in horses. Five mixed breed apparently healthy horses were administered oxytetracycline hydrochloride (20 mg/kg) intravenously and intramuscularly...

Fife, K.H.; Barbarash, R.A.; Rudolph, T.; Degregorio, B.; Roth, R., 1997: Valaciclovir versus acyclovir in the treatment of first-episode genital herpes infection. Results of an international, multicenter, double-blind, randomized clinical trial. The Valaciclovir International Herpes Simplex Virus Study Group. Valaciclovir, the L-valine ester prodrug of acyclovir, is much better absorbed than acyclovir and produces acyclovir exposures three to five times those attainable with the parent drug. To determine whether the improved bioavailability of valacicl...

Goldstein, J.; Dickson, D.W.; Rubenstein, A.; Woods, W.; Mincer, F.; Belman, A.L.; Davis, L., 1990: Primary central nervous system lymphoma in a pediatric patient with acquired immune deficiency syndrome. Treatment with radiation therapy. Primary central nervous system (CNS) lymphoma, an otherwise rare pediatric tumor, has been reported with increasing frequency in children with acquired immune deficiency syndrome (AIDS). With current therapy, the outcome of this disease is invaria...

Mian, M.; Aloisi, R.; Benetti, D.; Rosini, S.; Fantozzi, R., 1992: Potential role of heterologous collagen in promoting cutaneous wound repair in rats. The effect of native bovine tendon type-I collagen sponges (CONDRESS) on wound repair was evaluated by employing an experimental animal (rat) model which utilized subcutaneously implanted collagen and polyurethane sponges. Lesions were also create...

Oh, C.-Hyun; Lee, S.-Chul; Cho, J.-Hyuck, 2003: Synthesis and antibacterial activity of 1beta-methyl-2-(5-(N-substituted-2-hydroxy iminoethyl)pyrrolidin-3-ylthio)carbapenem derivatives. The synthesis of a new series of 1-methylcarbapenems having the substituted thiazolopyrrolidine moiety is described. Their in vitro antibacterial activities against both gram-positive and gram-negative bacteria were tested and the effect of substi...

Wei, C.; Dong, Y.; Lin, R., 2002: The actions of omapatrilat, vasopeptidase inhibitor , on cardiac hypertrophy and brain natriuretic peptide concentration in spontaneously hypertensive rats. The current study was designed to investigate the effects of omapatrilat, a vasopeptidase inhibitor (VPI), on plasma BNP level and cardiac function in spontaneously hypertensive rats (SHR). Studies were performed in SHR which received vehicle (n=8...

Paton A.M.; Aloysius S.K.D., 1982: The induction of nonpathogenic bacterial infections in plants. International Union Of Microbiological Societies 13th International Congress Of Microbiology; Boston, Mass , Usa, Aug 8-13, Xiv+182p American Society For Microbiology: Washington, D C , Usa Paper P79