+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evaluation of cooperative interactions between substructures of iso-1-cytochrome c using double mutant cycles

, : Evaluation of cooperative interactions between substructures of iso-1-cytochrome c using double mutant cycles. Biochemistry 42(36): 10659-10666

A double mutant cycle has been used to evaluate interaction energies between the global stabilizer mutation asparagine 52 --> isoleucine (N52I) in iso-1-cytochrome c and mutations producing single surface histidines at positions 26, 33, 39, 54, 73, 89, and 100. These histidine mutation sites are distributed through the four cooperative folding units of cytochrome c. The double mutant cycle starts with the iso-1-cytochrome c variant AcTM, a variant with no surface histidines and with asparagine at position 52. Isoleucine is added singly at position 52, AcTMI52 variant, as are the surface histidines, AcHX variants, where X indicates the histidine sequence position. The double mutant variants, AcHXI52, provide the remaining corner of the double mutant cycle. The stabilities of all variants were determined by guanidine hydrochloride denaturation and interaction energies were calculated between position 52 and each histidine site. Six of the seven double mutants show additive (AcH33I52, AcH39I52, AcH54I52, AcH89I52, and AcH100I52) stability effects or weak interaction energies (AcH73I52) of the histidine mutations and the N52I mutation, consistent with cooperative effects on protein folding and stability being sparsely distributed through the protein structure. The AcH26I52 variant shows a strong favorable interaction energy, 2.0 [plus or minus] 0.5 kcal/mol, between the N52I mutation in one substructure and the addition of His 26 to an adjacent substructure. The data are consistent with an entropic stabilization of the intersubstructure hydrogen bond between His 26 and Glu 44 by the Ile 52 mutation. Reprinted by permission of the publisher.


PMID: 12962490

DOI: 10.1021/bi034958t

Other references

Davido, B.; Fellous, L.; Lawrence, C.; Maxime, V.; Rottman, M.; Dinh, Aélien., 2017: Ceftazidime-Avibactam and Aztreonam, an Interesting Strategy To Overcome β-Lactam Resistance Conferred by Metallo-β-Lactamases in Enterobacteriaceae and Pseudomonas aeruginosa. Antimicrobial Agents and ChemoTherapy 61(9)

Stith, L.S.; Fisher, W.D.; Pressley, E.H., 1968: Upland results and spinning performance. Tabulated data are presented for yield trials, fibre quality and resistance to Verticillium wilt of numerous cotton varieties.

Zapf, K.; Purz, H.J.; Hilse, H., 1971: Vesicle membrane relations in hypothalamic synapses of the rat, shown by use of freeze etching. Acta Biologica et Medica Germanica 27(1): 225-228

Melville, RV., 1984: Goeride Ulmer, 1903 versus Trichostomatidae Rambur, 1842 (Insecta, Trichoptera): request for a ruling under Article 23d(ii). Z.N. (S.) 1456. Bulletin of Zoological Nomenclature, 412: 80-82

Nischk, D.; Merz, P.; Rusch, J., 2014: News from the Soteria--a phenomenologically inspired approach to the rehabilitation of social and daily living skills. Soteria is an alternative approach to the treatment of schizophrenia that focuses on human interaction ("being-with") within a residential community and the cautious use of neuroleptic medication. Despite forty years of promising clinica...

Stewart, K.J.; Seemans, C.M.; Lipis, P.H.; Mcfarland, L.D.; Weinhofer, J.J.; Brown, C.S., 1994: Should weight control be the primary message of heart health programs for elementary school children?. Circulation 90(4 PART 2): I473

Kang, M.; Chen, F.; Wu, S.; Yang, Y.; Bruggeman, C.; Charlet, L., 2011: Effect of pH on aqueous Se(IV) reduction by pyrite. Interaction of aqueous Se(IV) with pyrite was investigated using persistently stirred batch reactors under O2-free (<1 ppm) conditions at pH ranging from 4.5 to 6.6. Thermodynamic calculations, an increase in pH during the experiments, and spec...

Mikan, C.J.; Abrams, M.D., 1996: Mechanisms inhibiting the forest development of historic charcoal hearths in southeastern Pennsylvania. Charcoal production associated with the iron industry had a pervasive influence on forests during the 17th and 18th centuries in the mid-Atlantic region of the eastern United States. In a recent study we reported the altered forest composition and...

Madsbad S.; Edsberg B.; Bahnsen M.; Krarup T.; Hilsted J.; Christensen N.J.; Kuhl C., 1987: Effects of 6 weeks of strict metabolic control on glucose counterregulation and recognition of hypoglycemic symptoms in type 1 patients. Acta Endocrinologica Supplementum 115(282): 39

White William Arthur, 1957: Hypothesis concerning the origin of structures in argillaceous rocks. Geological Society of America Bulletin 68(12, Part 2): 1812