geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Functional Domains of FOXJ2


, : Functional Domains of FOXJ2. Journal of Molecular Biology 329(4): 1-44

FOXJ2 is a fork head transcriptional activator, the expression of which starts very early in embryonic development and it is distributed widely in the adult. Here, we describe the characterization of domains that are important for its function. FOXJ2 is localized constitutively at the nucleus of the cell. Two tyrosine residues and a stretch of basic amino acid residues at the N and C-terminal ends of the fork head domain, respectively, are important for its nuclear targeting. These residues are conserved strongly among all members of the fork head family, suggesting that they could be involved in the nuclear translocation mechanism of all fork head factors. In addition to the AB domain, we have found, at least, two other transactivation domains: Domain I, at the N terminus, and the H/P domain, rich in histidine and proline residues. Although the AB domain shows the strongest transactivation capacity, all three domains are required for full FOXJ2 transcriptional activity. Furthermore, a fourth region rich in proline and glutamine residues and with no intrinsic transactivation function, the P/Q domain, appears to play an important role in the FOXJ2-mediated transactivation mechanism. Although FOXJ2 can be phosphorylated in two serine residues, this post-translational modification did not appear to be essential for transactivation. Finally, we have found that the W2 wing of the fork head domain of FOXJ2 is dispensable for specific DNA binding, although it could have a weak stabilizing role for the DNA-FOXJ2 complex. Reprinted by permission of the publisher.

US$19.90

PMID: 12787665

DOI: 10.1016/s0022-2836(03)00524-2


Other references

Zheng, Y.; Liu, J.; Wang, D.; Yang, R., 2012: Laser scanning measurements on trees for logging harvesting operations. Logging harvesters represent a set of high-performance modern forestry machinery, which can finish a series of continuous operations such as felling, delimbing, peeling, bucking and so forth with human intervention. It is found by experiment that...

Weinstein, G.D., 1983: Commentary: three decades of folic acid antagonists in dermatology. Archives of Dermatology 119(6): 525-527

Anonymous, 2012: Peroxisome Proliferator-Activated Receptor-gamma Regulates Inflammation and Renin-Angiotensin System Activity in the Hypothalamic Paraventricular Nucleus and Ameliorates Peripheral Manifestations of Heart Failure. Activation of peroxisome proliferator-activated receptor (PPAR)-, a nuclear transcription factor, has been shown to inhibit the production of proinflammatory cytokines and, in peripheral tissues, to downregulate the renin-angiotensin system. PPAR-...

Mironov, S.L.; Richter, D.W., 1998: L-type Ca2+ channels in inspiratory neurones of mice and their modulation by hypoxia. 1. Whole-cell (ICa) and single Ca2+ channel currents were measured in inspiratory neurones of neonatal mice (4-12 days old). During whole-cell recordings, ICa slowly declined and disappeared within 10-20 min. The run-down was delayed during hypoxi...

N.P.nella-Riera; A.V.larde; A.D.lmau; E.F. brega; M.F.nt i Furnols; M.G.spert; J.S.ler; J.T.bau; M.A.O.iver; M.G.l, 2009: Effect of magnesium sulphate and l-tryptophan and genotype on the feed intake, behaviour and meat quality of pigs. Livestock Science 124(1-3): 0-287

Aufranc, O.E.; Jones, W.N.; Harris, W.H., 1963: Fracture Of The Month. 42. Fracture Dislocation Of The Hip. JAMA 186: 1160-1163

Thines, L.; Delmaire, C.; L.G.rs, D.; Pruvo, J.P.; Lejeune, J.P.; Lehmann, P.; Francke, J.P., 2006: MRI localization of paraclinoid carotid aneurysms. The distal dural ring plane (DDRP) separates the intracavernous from the supracavernous paraclinoid internal carotid artery. The purpose of this MRI protocol is to evaluate the position of this plane for the characterization of paraclinoid aneurys...

Heroux, O., 1958: Weights and composition of muscles of warm-and cold-acclimated rats. After cold acclimation at 6[degree] C white rats have a smaller muscle mass than control animals of the same age kept at 30[degree]C. Water and protein determinations in soleus and gastrocnemius muscles as well as in the whole muscular mass reveal...

Valles, E.; Mocquot, G., 1972: Technique of rennet preparation used in the traditional manufacture of Gruyere de Comte and Emmental cheeses. In this study of the traditional method of rennet preparation Lactobacillus helveticus culture was added to whey that had been heated for 5 min at 75-78 deg C or deproteinized after heating at 93-95 deg C; 5-100 g dried vells were added to the whe...

Winchurch, R.A.; Cimino, E.F.; Mardiney, M.R., 1977: In vitro discordance between actual lymphocyte proliferation and thymidine incorporation on exposure of human lymphocytes to specific antigen or allogeneic cells. Transplantation Proceedings 9(1): 793-796