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Functional Domains of FOXJ2

, : Functional Domains of FOXJ2. Journal of Molecular Biology 329(4): 1-44

FOXJ2 is a fork head transcriptional activator, the expression of which starts very early in embryonic development and it is distributed widely in the adult. Here, we describe the characterization of domains that are important for its function. FOXJ2 is localized constitutively at the nucleus of the cell. Two tyrosine residues and a stretch of basic amino acid residues at the N and C-terminal ends of the fork head domain, respectively, are important for its nuclear targeting. These residues are conserved strongly among all members of the fork head family, suggesting that they could be involved in the nuclear translocation mechanism of all fork head factors. In addition to the AB domain, we have found, at least, two other transactivation domains: Domain I, at the N terminus, and the H/P domain, rich in histidine and proline residues. Although the AB domain shows the strongest transactivation capacity, all three domains are required for full FOXJ2 transcriptional activity. Furthermore, a fourth region rich in proline and glutamine residues and with no intrinsic transactivation function, the P/Q domain, appears to play an important role in the FOXJ2-mediated transactivation mechanism. Although FOXJ2 can be phosphorylated in two serine residues, this post-translational modification did not appear to be essential for transactivation. Finally, we have found that the W2 wing of the fork head domain of FOXJ2 is dispensable for specific DNA binding, although it could have a weak stabilizing role for the DNA-FOXJ2 complex. Reprinted by permission of the publisher.


PMID: 12787665

DOI: 10.1016/s0022-2836(03)00524-2

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