+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Genome-wide analysis of vaccinia virus protein-protein interactions

, : Genome-wide analysis of vaccinia virus protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America 97(9): 4879-4884

To detect interactions between proteins of vaccinia virus, we carried out a comprehensive two-hybrid analysis to assay every pairwise combination. We constructed an array of yeast transformants that contained each of the 266 predicted viral ORFs as Gal4 activation domain hybrid proteins. The array was individually mated to transformants containing each ORF as a Gal4-DNA-binding domain hybrid, and diploids expressing the two-hybrid reporter gene were identified. Of the approximately 70,000 combinations, we found 37 protein-protein interactions, including 28 that were previously unknown. In some cases, e.g., late transcription factors, both proteins were known to have related roles although there was no prior evidence of physical associations. For some other interactions, neither protein had a known role. In the majority of cases, however, one of the interacting proteins was known to be involved in DNA replication, transcription, virion structure, or host evasion, thereby providing a clue to the role of the other uncharacterized protein in a specific process.


PMID: 10781095

DOI: 10.1073/pnas.080078197

Other references

Vyas, A.; Syeda, K.; Ahmad, A.; Padhye, S.; Sarkar, F.H., 2012: Perspectives on medicinal properties of mangiferin. Mango tree, Mangifera indica, has been cultivated in India and several other tropical countries for centuries, and it is a good source of compound 'mangiferin'. Mangiferin's xanthonoid structure with C-glucosyl linkage and polyhydro...

Nensey, Y.M.; Arlow, F.L.; Majumdar, A.P., 1995: Aging. Increased responsiveness of colorectal mucosa to carcinogen stimulation and protective role of folic acid. Recent investigations have demonstrated a protective role for folic acid in dysplasia and neoplasia, through an unknown mechanism. The current study was designed to evaluate whether the protective role of folic acid is due, in part, to its antipro...

Chang, X.; Patiño, R.; Yoshizaki, G.; Thomas, P.; Lee, V.H., 2000: Hormonal regulation and cellular distribution of connexin 32.2 and connexin 32.7 RNAs in the ovary of Atlantic croaker. The in vitro effects of human chorionic gonadotropin (hCG) on ovarian connexin (Cx) 32.2 and 32.7 RNA levels and ovarian follicle maturation were assessed, and the cellular distribution of Cx transcripts in the ovary was determined. hCG caused a c...

Killingbeck, K.T., 1996: Nutrients in senesced leaves: keys to the search for potential resorption and resorption proficiency. The concept of nutrient resorption proficiency, the minimum level to which a plant can reduce an element in senescing leaves, is introduced. Data from 89 species of woody perennials show that resorption proficiencies are convergent across terrestr...

Wright, S.J.; Ulph, F.; Dharni, N.; Payne, K., 2017: Eliciting Preferences for Information Provision in Newborn Bloodspot Screening Programs. The national newborn bloodspot screening programs (NBSPs) are continually expanding to screen for more conditions. To quantify parents' preferences for information and the way in which this is provided in example NBSPs. A hybrid choice experi...

Bennett, A.G., 1990: Sagittae of toroidal surfaces. Optometry and Vision Science 67(11): 850-853

Wang Yuwang; Qin Kezhang; Tan Yanggeng; Hou Zengqian, 2000: The Derni Cu-Co massive sulfide deposit, Qinghai Province, China; ultramafic volcanic-hosted submarine-exhalative mineralization. The large Derni Cu-Co (-Zn) deposit in Qinghai Province exhibits unusual geological features, and its genesis is controversial. Based on previous research and detailed field investigations, petrographic studies of the host volcanic rocks, isotope...

Tessier, A.M.; Nolot, P.; Hoffelt, J.; Gaugain, B.; Delaveau, P., 1977: Chemical, pharmacological, and clinical study of traditional and modern chewing gum. III. Elastomers of Apocynacea and Sapotacea used in chewing gum. Annales Pharmaceutiques Francaises 35(1-2): 37-43

Anonymous, 2001: Energy metabolism and carcinogenesis. Science of the Total Environment 271(1-3): 169-170

Willemse, G.R.W.M.; Smit, F.; Cuijpers, P.; Tiemens, B.G., 2004: Minimal-contact psychotherapy for sub-threshold depression in primary care. Randomised trial. Background Sub-threshold depression is a prognostic variable for major depression. Interventions in subthreshold depression may prevent the onset of new cases of major depression. Aims To examine the effects of minimal-contact psychotherapy in pri...