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HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC


, : HPTLC and reflectance mode densitometry of anthocyanins in Malva silvestris L.: a comparison with gradient-elution reversed-phase HPLC. Journal of Pharmaceutical and Biomedical Analysis 14(1-2): 203-211

Aqueous alcoholic mallow flower extracts were analyzed both by HPTLC-densitometry in the reflectance mode at 530 nm and by reversed-phase HPLC with gradient elution. For the mallow flower anthocyanins the best chromatographic resolution was obtained by HPLC, which revealed only two main compounds, confirmed by FAB-MS: malvidin 3,5-O-diglucoside (malvin) and malvidin 3-O-(6''-O-malonylglucoside)-5-O-glucoside. The HPTLC densitometric method on cellulose plates provides accuracy, reproducibility and selectivity for the quantitative analysis of the anthocyanins and this method was shown to be much more sensitive than the HPLC-DAD system, at 530 nm. Both methods give comparable quantitative results for total anthocyanins when applied to mallow flowers from two different sources: Italy and Albania.

US$19.90

PMID: 8833983

DOI: 10.1016/0731-7085(95)01632-5


Other references

Hazen R.M.; Finger L.W., 1977: Compression models for oxides and silicates. Abstracts with Programs - Geological Society of America 9(7): 1008-1009

Grisold, W.; Karnel, F.; Kumpan, W.; Hitzenberger, P.; Zifko, U., 1999: Iliac artery aneurysm causing isolated superior gluteal nerve lesion. Lesions of the superior gluteal nerve (SGN) lead to weakness of hip abduction, manifesting itself as a gait abnormality, with contralateral tilting of the pelvis with each step. Causes are numerous and may occur at different anatomical locations b...

Wiecek, E.; Jackson, M.Lou.; Dakin, S.C.; Bex, P., 2012: Visual search with image modification in age-related macular degeneration. AMD results in loss of central vision and a dependence on low-resolution peripheral vision. While many image enhancement techniques have been proposed, there is a lack of quantitative comparison of the effectiveness of enhancement. We developed a...

Mutum, S.S.; Sharma, L.D.; Singh, T.D.; Dutta, S.; Singh, S., 1988: Lymphocytoma cutis--a case report. Indian Journal of Pathology & Microbiology 31(3): 251-253

Gu, L.; Waliany, S.; Kane, S.E., 2009: Darpp-32 and its truncated variant t-Darpp have antagonistic effects on breast cancer cell growth and herceptin resistance. Herceptin (trastuzumab) is a humanized monoclonal antibody that is approved for the treatment of metastatic breast cancer patients whose tumors overexpress Her2 (erbB2/neu). Up to 70% of Her2-positive breast cancers demonstrate a response to Herce...

Ramfjord, S.P., 1974: Periodontal aspects of restorative dentistry. Journal of Oral Rehabilitation 1(2): 107-126

Manzoni, L.; Rui, D.B., 1934: Review of the principal phytopathological cases observed in Conegliano in 1933. Infection of grapes by Plasmopara viticola was related to the no. of days in the latter half of May when the temp. was below 10[degree]C. The use of aluminum sulphate and potash alum, either alone or with bordeaux mixture, did not improve the cont...

Branciforti, S.; Prignacchi, V., 1952: Streptomycin in therapy of osteoarticular tuberculosis in adults. Minerva Ortopedica 3(4): 226-238

Yoshida, K-ichi.; Kanaoka, S.; Kajimura, M.; Kataoka, H.; Takahira, K.; Osawa, S.; Sano, M.; Hishida, A., 2003: A Japanese case of familial Mediterranean fever with family history demonstrating a mutation in MEFV. We describe a 17-year-old woman with a family history of FMF who suffered from recurrent fever accompanied by pains in the left chest and abdomen. During a five-year period she experienced attacks about once every six months. The metaraminol provo...

Manhar, A.K.; Bashir, Y.; Saikia, D.; Nath, D.; Gupta, K.; Konwar, B.K.; Kumar, R.; Namsa, N.D.; Mandal, M., 2017: Corrigendum to "Cellulolytic potential of probiotic Bacillus Subtilis AMS6 isolated from traditional fermented soybean (Churpi): An in-vitro study with regards to application as an animal feed additive" [Microbiol. Res. 186-187 (2016) 62-70. Microbiological Research 198: 56-57