+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes

, : Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes. Microbiology 145: 3139-3146

Haemolysin BL (HBL) is a Bacillus cereus toxin composed of a binding component, B, and two lytic components, L(1) and L(2). HBL is also the enterotoxin responsible for the diarrhoeal food poisoning syndrome caused by several strains of B. cereus. The three genes encoding the HBL components constitute an operon and are transcribed from a promoter 608 bp upstream of the hblC translational start site. The first gene of the hbl operon, hblC, in the B. cereus type strain, ATCC 14579, was inactivated in this study. Inactivation of hblC strongly reduced both the enterotoxigenic activity of B. cereus ATCC 14579 and the haemolytic activity against sheep erythrocytes, while maintaining full haemolytic activity against human erythrocytes.


PMID: 10589721

DOI: 10.1099/00221287-145-11-3139

Other references

Ahmed, K.; Anderson, O.; Jawad, M.; Tierney, T.; Darzi, A.; Athanasiou, T.; Hanna, G.B., 2015: Design and validation of the surgical ward round assessment tool: a quantitative observational study. Ward round skills are essential for the best management of surgical inpatients, but assessment of their quality has received inadequate attention. This study aims to design and validate the surgical ward round assessment tool (SWAT). We used modif...

Gschossmann, J.M.; Adam, B.; Liebregts, T.; Best, J.; Bechmann, L.; Hilken, G.; Callies, R.; Buenger, L.; Gerken, G.; Holtmann, G., 2002: Influence of the sexual hormones on the post-inflammatory sensory function in female rats. Gastroenterology 122(4 Suppl 1): A-526, April

Huang, S.; Chen, L.; Li, J., 1999: The occurrence and control strategy of main pests on Cinnamomun cassia. Senlin Bingchong Tongxun. June 30; 182: 37-39

Jenssen, K.; Sewald, K.; Sewald, N., 2004: Synthesis of marimastat and a marimastat conjugate for affinity chromatography and surface plasmon resonance studies. Immobilized small-molecule inhibitors are suited for enrichment of biomolecules by affinity chromatography, as it is shown for metalloproteinases and an immobilizable derivative of the hydroxamate-type inhibitor marimastat. A new asymmetric synthe...

Greenham, R., 1978: Anaemia and Schistosoma haematobium infection in the north-eastern Province of Kenya. The haemoglobin levels of 787 Somali people in north-eastern Kenya were measured. Severe anaemia was very common in both sexes of all ages. Possible causative factors are discussed. Adolescent boys had particularly low haemoglobin values (in one a...

McMillan, J.N.; Sia, R.A.; Bardes, E.S.; Lew, D.J., 1999: Phosphorylation-independent inhibition of Cdc28p by the tyrosine kinase Swe1p in the morphogenesis checkpoint. The morphogenesis checkpoint in budding yeast delays cell cycle progression in G(2) when the actin cytoskeleton is perturbed, providing time for cells to complete bud formation prior to mitosis. Checkpoint-induced G(2) arrest involves the inhibiti...

Fitch A.A.; Lloyd E.H., 1951: The precision of travel-time curves in the seismic method of geophysical surveying. Sources of discrepancy between results of seismic surveys and actual subsurface conditions are discussed, with special reference to the effect of random erros on dip determinations made by both refraction and reflection methods.

Neumann, S., 1977: Short time planning in land reclamation cooperative. Kooperation 11 (2) 65-67

Talysin, T.T., 1941: Influence of parasitic Worms on the secretory function of the stomach and small intestne in Dog. CR Acad Sci Moscou, 81: 413-416

McEward, LR.; Carson, SF., 1987: Variation in egg organic content and its relationship with egg size in the starfish Solaster stimpsoni. Marine Ecology Progress Series, 372-3: 159-169