geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular flexibility and discontinuous translocation of a non-templated polymerase


, : Molecular flexibility and discontinuous translocation of a non-templated polymerase. Journal of Molecular Biology 337(4): 843-856

Little is known regarding the translocation of non-templated nucleic acid polymerases with respect to single-stranded primers. VP55, the vaccinia virus poly(A) polymerase, translocates as it processively adds a [similar]3-7 adenylate tail to primers possessing only three ribouridylate residues (as an (rU)2-N15-rU motif), and a [similar]25-30 adenylate tail to primers that are more U-rich. Here, three models were addressed for the translocation of VP55 with respect to its primer, namely: (a) rigid protein/rigid nucleic acid; (b) flexible protein/rigid nucleic acid; (c) rigid protein/flexible nucleic acid. Analysis of free and covalently VP55-attached primers favored either (b) or a version of (c) incorporating a passive steric block, and suggested two regions of relative motion between polymerase and primer. Inclusion of a 6 nt uridylate-rich patch at the primer 3' end switched the polymerase from [similar]3-7 nt to [similar]25-30 nt tail addition without affecting initial binding affinity. By synthesizing this patch as a (rU/dC) pool, discontinuous polymerase movements could be detected. Reprinted by permission of the publisher.

US$19.90

PMID: 15033355

DOI: 10.1016/j.jmb.2004.01.058


Other references

Reumert, M., 1980: A review of hospital journals. Ugeskrift for Laeger 142(5): 337-341

K.F.ji-ta; T.K.tsura; Y.T.inosho, 2004: Electrical conductivity measurement of granulite under mid- to lower crustal pressure-temperature conditions. Geophysical Journal International 157(1): 79-86

Moleleki, N.; van Heerden, S.W.; Wingfield, M.J.; Wingfield, B.D.; Preisig, O., 2003: Transfection of Diaporthe perjuncta with Diaporthe RNA virus. Diaporthe perjuncta is a pathogen of grapevines worldwide. A positive-strand RNA virus, Diaporthe RNA virus (DaRV), occurs in hypovirulent isolates of this fungus. A virus-free isolate from a South African grapevine was transfected with in vitro-t...

Hejduková, B.; Hosseini, N.; Johnels, B.; Ingvarsson, P.E.; Steg, Göran.; Olsson, T., 2003: Manual transport in Parkinson's disease. We analyze hand dexterity in Parkinson's disease patients (PD) and control subjects using a natural manual transport task (moving an object from one place to another). Eight PD patients and 10 control subjects carried out the task repeatedly...

Wiegand, M.; Berger, M.; Zulley, J.; Lauer, C.; von Zerssen, D., 1987: The influence of daytime naps on the therapeutic effect of sleep deprivation. Biological Psychiatry 22(3): 389-392

Spieth, M.E., 2002: Peer reviewers: reviewing manuscripts. Ajr. American Journal of Roentgenology 179(6): 1645-1645

Matsushita, M.; Matsudaira, R.; Ikeda, K.; Nawata, M.; Tamura, N.; Takasaki, Y., 2010: Anti-proteasome activator 28alpha is a novel anti-cytoplasmic antibody in patients with systemic lupus erythematosus and Sjögren's syndrome. We evaluated the extent to which anti-proteasome activator (PA) 28alpha antibodies act as anti-cytoplasmic antibodies in systemic lupus erythematosus (SLE) and Sjögren's syndrome (SS). Sera from 46 SLE patients without SS, 11 SLE patients wi...

Dzhaparidze, S.S.; Dzhaparidze, S.A., 2005: Clinico-morphological value of modified nephropexy method. We have observed 28 patients operated by modified method of nephropexy. Principle of this method is the fixation of kidney on 11 or 12 rib with silk floss #5 or #6. Floss is passed subcapsularly around lower pole of the kidney and separately on an...

Byler, D.L.; Grageda, M.R.; Halstead, E.Scott.; Kanekar, S., 2014: Rapid onset of hippocampal atrophy in febrile-infection related epilepsy syndrome (FIRES). Febrile infection-related epilepsy syndrome (FIRES) is a catastrophic and usually refractory epilepsy syndrome that occurs after a febrile illness in previously normal children. The pathogenesis of the syndrome is unknown, and the diagnosis is typ...

Glendenning, P.; Chew, G.T.; Seymour, H.M.; Gillett, M.J.; Goldswain, P.R.; Inderjeeth, C.A.; Vasikaran, S.D.; Taranto, M.; Musk, A.A.; Fraser, W.D., 2009: Serum 25-hydroxyvitamin D levels in vitamin D-insufficient hip fracture patients after supplementation with ergocalciferol and cholecalciferol. Vitamin D insufficiency is commonly associated with hip fracture. However, the equipotency of ergocalciferol and cholecalciferol supplementation in this patient group has not been studied in a randomized trial using high-performance liquid chromat...