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Molecular flexibility and discontinuous translocation of a non-templated polymerase

, : Molecular flexibility and discontinuous translocation of a non-templated polymerase. Journal of Molecular Biology 337(4): 843-856

Little is known regarding the translocation of non-templated nucleic acid polymerases with respect to single-stranded primers. VP55, the vaccinia virus poly(A) polymerase, translocates as it processively adds a [similar]3-7 adenylate tail to primers possessing only three ribouridylate residues (as an (rU)2-N15-rU motif), and a [similar]25-30 adenylate tail to primers that are more U-rich. Here, three models were addressed for the translocation of VP55 with respect to its primer, namely: (a) rigid protein/rigid nucleic acid; (b) flexible protein/rigid nucleic acid; (c) rigid protein/flexible nucleic acid. Analysis of free and covalently VP55-attached primers favored either (b) or a version of (c) incorporating a passive steric block, and suggested two regions of relative motion between polymerase and primer. Inclusion of a 6 nt uridylate-rich patch at the primer 3' end switched the polymerase from [similar]3-7 nt to [similar]25-30 nt tail addition without affecting initial binding affinity. By synthesizing this patch as a (rU/dC) pool, discontinuous polymerase movements could be detected. Reprinted by permission of the publisher.


PMID: 15033355

DOI: 10.1016/j.jmb.2004.01.058

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