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Rhodopsin kinase: expression in mammalian cells and a two-step purification

, : Rhodopsin kinase: expression in mammalian cells and a two-step purification. Proceedings of the National Academy of Sciences of the United States of America 97(7): 04-9

The expression and purification of rhodopsin kinase (RK) are reported. The expression of the RK gene in the yeast Pichia pastoris, COS 1 cells, and an HEK293 stable cell line was compared. The use of the pCMV5 vector to express RK in COS cells was the most satisfactory method, and the expressed enzyme with an N-terminal histidine tag was purified using a 2-step protocol. The purified enzyme demonstrated correct posttranslational modifications and a somewhat broader pH vs. catalytic activity profile in comparison with the wild-type enzyme.


PMID: 10737781

DOI: 10.1073/pnas.97.7.3004

Other references

Pisano, E., 1964: Clinical Analysis Of The Ishihara Tables,3th Edition. Annali di Ottalmologia E Clinica Oculistica 90: 813-816

Attout, T.; Babayan, S.; Hoerauf, A.; Taylor, D.W.; Kozek, W.J.; Martin, C.; Bain, O., 2005: Blood-feeding in the young adult filarial worms Litomosoides sigmodontis. In this study with the filarial model Litomosoides sigmodontis, we demonstrate that the worms ingest host red blood cells at a precise moment of their life-cycle, immediately after the fourth moult. The red blood cells (RBC) were identified micros...

Liepsch D.; Moravec S.; Rastogi A.K.; Vlachos N.S., 1982: Measurement and calculations of laminar flow in a 90 degree bifurcation. Measurements and numerical calculations of laminar flow in a plane bifurcation are presented. The corresponding 2-dimensional steady flow Navier-Stokes equations are solved by a finite-difference procedure employing pressure and velocit...

Basile A.C., 1988: Clinical implications of nutrient drug nutrient interferences in geriatrics. Folha Medica 97(3): 187-193

Du-Preez, J.S., 1976: Body measurements carcass and organ mass of mammals from the etosha national park. The measurements and mass of zebra Equus burchelli antiquorum, springbok Antidorcas marsupialis, gemsbok Oryx gazella and wildebeest Connochaetus [Connochaetes?] taurinus, as well as the mass of certain organs and the hides from these animals are...

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Sclater, W.L., 1925: Obituary: James Davidson; Frank Evers Bed-dard; Eugene Pintard Bicknell; Ned Hollister. Ibis London, 919-923

Niu, J.; Li, Z.; Peng, B.; Chiao, P.J., 2003: Identification of an autoregulatory feedback pathway involving interleukin-1alpha in induction of constitutive NF-kappaB activation in pancreatic cancer cells. We previously reported that NF-kappaB is constitutively activated in most human pancreatic cancer tissues and cell lines but not in normal pancreatic tissues and immortalized pancreatic ductal epithelial cells. IkappaBalphaM-mediated inhibition of...

Odiase, G.I., 1978: Ten leading causes of death among in-patients of the University of Benin Teaching Hospital in the year 1974. Nigerian Medical Journal 8(3): 242-248