geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Selective up-regulation of dopamine D1 receptors in dendritic spines by NMDA receptor activation


, : Selective up-regulation of dopamine D1 receptors in dendritic spines by NMDA receptor activation. Proceedings of the National Academy of Sciences of the United States of America 99(3): 1661-1664

Glutamate, by activating N-methyl-d-aspartate (NMDA) receptors, alters the balance between dopamine D1 and D2 receptor signaling, but the mechanism responsible for this effect has not been known. We report here, using immunocytochemistry of primary cultures of rat neostriatal neurons, that activation of NMDA receptors recruits D1 receptors from the interior of the cell to the plasma membrane while having no effect on the distribution of D2 receptors. The D1 receptors were concentrated in spines as shown by colocalization with phalloidin-labeled actin filaments. The effect of NMDA on D1 receptors was abolished by incubation of cells in calcium-free medium and was mimicked by the calcium ionophore ionomycin. Recruitment of D1 receptors from the interior of the cell to the membrane was confirmed by subcellular fractionation. The recruited D1 receptors were functional as demonstrated by an increase in dopamine-sensitive adenylyl cyclase activity in membranes derived from cells that had been pretreated with NMDA. These results provide evidence for regulated recruitment of a G protein-coupled receptor in neurons, provide a cell biological basis for the effect of NMDA on dopamine signaling, and reconcile the conflicting hyperdopaminergic and hypoglutamatergic hypotheses of schizophrenia.

US$19.90

PMID: 11818555

DOI: 10.1073/pnas.032654599


Other references

Cane, R.D.; Rivlin, M.; Buchanan, N., 1976: The management of septic abortion in an intensive care unit. The value of ICU treatment in the management of septic abortion has been assessed. Of patients subjected to hysterectomy, the mortality is now 20%, as opposed to 37% prior to the advent of ICU. It would therefore appear that these patients can ben...

Marius, P.; Alvis, S.J.; East, J.Malcolm.; Lee, A.G., 2005: The interfacial lipid binding site on the potassium channel KcsA is specific for anionic phospholipids. Lipid binding to the potassium channel KcsA from Streptomyces lividans has been studied using quenching of the fluorescence of Trp residues by brominated phospholipids. It is shown that binding of phospholipids to nonannular lipid binding sites on...

Pizzorno, G.; Sokoloski, J.A.; Cashmore, A.R.; Moroson, B.A.; Cross, A.D.; Beardsley, G.P., 1991: Intracellular metabolism of 5,10-dideazatetrahydrofolic acid in human leukemia cell lines. 5,10-Dideazatetrahydrofolic acid (DDATHF) is a new potent antitumor agent that specifically inhibits purine biosynthesis, primarily through inhibition of glycinamide ribonucleotide transformylase, the first of the tetrahydrofolate-requiring enzyme...

Vuadens, P.; Regli, F., 1995: Eosinophil meningoradiculitis due to Angiostrongylus cantonensis. The authors report the case of a 46-year-old woman who presented with an eosinophilic meningoradiculits after a trip to Tahiti. Because of the type of presentation of the symptomatology, the CSF findings, and the spontaneously favourable outcome,...

Oxley, D.; Currie, G.; Bacic, A., 2006: Monosaccharide composition analysis: alditol acetates. Csh Protocols 2006(1): -

Verena Maria Erian, Eva Messenlechner, Raimund Eller, 2012: Steuerabkommen mit der Schweiz Freund oder Feind?. Heuer im April hat unser Ministerrat das umstrittene, gelobte, kritisierte, ersehnte, verdammte, erw nschte, gef rchtete - kurzum das vieldiskutierte Steuerabkommen mit der Schweiz beschlossen. Was es damit auf sich hat, erfahren Sie hier.

Tsakiris, D.A., 2014: Direct oral anticoagulants--interference with laboratory tests and mechanism of action. Direct oral anticoagulants (DOACs) have been established and already registered for clinical use on a wide basis in the United States and Europe. Different from the vitamin K antagonists (VKA), their mode of action is based on the direct inhibitio...

Schonberger, J.P.A.M.; Bredee, J.J.; Tjian, D.; Everts, P.A.M.; Wildevuur, C.R.H., 1993: Intraoperative predonation contributes to blood saving. The merits of reinfusing prebypass-removed autologous blood (intraoperative predonation) to salvage blood and improve postoperative hemostasis are still debated, specifically for patients at a higher risk for bleeding. To evaluate the effect of in...

Najean, Y., 1987: The iatrogenic leukaemias induced by radio- and/or chemotherapy. A short review, limited to recently published series of data, has been compiled on the 'therapy-induced' secondary malignancies. Their frequency, peak of incidence, haematological and clinical criteria, the influence of age, treated prim...

Deschamps, P.N., 1961: The warning signs of myocardial infarct. La Presse Thermale et Climatique 98: 179-180