geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Sensitive determination of zearalenone and alpha-zearalenol in barley and Job's-tears by liquid chromatography with fluorescence detection


, : Sensitive determination of zearalenone and alpha-zearalenol in barley and Job's-tears by liquid chromatography with fluorescence detection. Journal of Aoac International 76(5): 1006-1009

A sensitive and reliable method for liquid chromatographic (LC) determination of zearalenone and alpha-zearalenol in barley and Job's-tears was investigated. The method by which these toxins were determined involves addition of an internal standard (zearalenone 6'-oxime) to barley and Job's-tears samples. Extracts from grain samples were cleaned up by passage through chromatography on piperidinohydroxypropyl Sephadex LH-20 as a lipophilic gel. Individual toxins were resolved by LC on a reversed-phase (ODS) column with fluorescence detection. The detection limit is estimated to be 0.2 ng for zearalenone and alpha-zearalenol standards. Known amounts of zearalenone and alpha-zearalenol (25-1250 ng) were added to a barley sample (5 g). Average recoveries for alpha-zearalenol and zearalenone, respectively, ranged from 96 to 102% (mean CV, 3.6%) and from 96 to 103% (mean CV, 3.3%). This method is applicable to determination of alpha-zearalenol and zearalenone in barley and Job's-tears with satisfactory sensitivity and accuracy.

US$29.90

PMID: 8241805


Other references

Buchanan D.L., 1982: Western resources of platinum-group metals, nickel, and chromium; their production and future demand. Pages 259-262 1982

Aguiar, R.O.; Viegas, F.C.; Fernandez, R.Y.; Trudell, D.; Haghighi, P.; Resnick, D., 2007: The prepatellar bursa: cadaveric investigation of regional anatomy with MRI after sonographically guided bursography. The purpose of this study was to use MRI and anatomic correlation in cadavers to show the macroscopic anatomic configuration of the prepatellar bursa. MRI of the prepatellar bursa of nine cadaveric knees was performed after sonographically guided...

Pough Frederick H., 1992: Fluorite, Part III. Lapidary Journal 45(12): 14, 16

Sakuma, T., 1984: Strains of varicella-zoster virus resistant to 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil. Strains of varicella-zoster virus resistant to 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil (BV-araU) were isolated from varicella-zoster virus-infected Vero cells which were pulse-treated with 5-iododeoxyuridine or 5-bromodeoxyuridine or bo...

Moya Moreno Hector; Hernandez Lascares Delfino, 1995: Building a data base system for the systematics of a paleontologic collection. Memoria de la : nion Nacional de Colecciones Paleontologicas. 3; Pages 11-13. 1995.

Dirks Volker; Smith Nigel; Kneale Robert, 2001: A case study on using AVO walkaway VSP data; Barrow Subbasin, Western Australia. Preview: ages 82. 2001.

Hodgson, K.J., 2005: Canine or chameleon (revisited): a never-ending challenge in a perpetually changing world. Vascular 13(2): 69-76

Wykoff, C.C.; Lam, B.L.; Brathwaite, C.D.; Biegel, J.A.; McKeown, C.A.; Rosenblum, M.K.; Allewelt, H.B.; Sandberg, D.I., 2008: Atypical teratoid/rhabdoid tumor arising from the third cranial nerve. An otherwise healthy 6-week-old girl who presented with an isolated left third cranial nerve palsy underwent MRI that revealed an enhancing mass intrinsic to the left third cranial nerve. Rapid enlargement of the lesion over 1 month led to subtota...

Podesta, G.P.; Nunez, L.; Villanueva, C.A.; Skansi, M.A., 2004: Estimating daily solar radiation in the Argentine Pampas. Solar radiation is an important input to crop growth models used for risk management and assessment purposes. Methods are explored to estimate daily solar radiation in the Argentine Pampas, one of the most important agricultural areas in the world...

Tubich, G.E., 1964: New Materials And Processes Create New Liabilities For The Foundry. Industrial Medicine & Surgery 33: 79-85