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Synthesis of viral DNA and late capsid protein L1 in parabasal spinous cell layers of naturally occurring benign warts infected with human papillomavirus type 1

, : Synthesis of viral DNA and late capsid protein L1 in parabasal spinous cell layers of naturally occurring benign warts infected with human papillomavirus type 1. Virology 268(2): 281-293

We investigated human papillomavirus type 1 (HPV1)-specific transcription, viral DNA replication, and viral protein expression in naturally occurring benign tumors by in situ hybridization, 5-bromodeoxyuridine (BrdU) incorporation, and immunohistochemistry and obtained results different from other HPV-infected benign tumors characterized so far. Moderate amounts of transcripts with a putative coding potential for E6/E7, E1, and E2 were demonstrated from the first subrabasal cell layer throughout the stratum spinosum and granulosum. In addition very large amounts of E4 and L1 transcripts were present in the same epithelial layers. This finding was substantiated by the demonstration of L1 and E4 protein already in the bottom-most spinous cell layer. Furthermore massive amplification of the viral DNA as measured by BrdU incorporation and different methods of in situ hybridization took place in the lowest 5 to 10 suprabasal cell layers. These findings are in contrast to the assumption that late gene expression and viral DNA synthesis are restricted to the more differentiated cell layers of the epithelium and point to differences in the regulation of the vegetative life cycle between different papillomavirus types. Copyright 2000 Academic Press.


PMID: 10704337

DOI: 10.1006/viro.1999.0174

Other references

Costello, P.C.; Del Maestro, R.F.; Stetler-Stevenson, W.G., 1994: Gelatinase A expression in human malignant gliomas. Annals of the New York Academy of Sciences 732: 450-452

Zhang, H-Feng.; Xu, L-Yan.; Li, E-Min., 2014: A family of pleiotropically acting microRNAs in cancer progression, miR-200: potential cancer therapeutic targets. Recently, a group of microRNAs (miRNAs), the miR-200 family (miR-200s) has been found to be deregulated in multiple types of cancers, in which this family of miRNAs was demonstrated to play a pivotal role in tumor initiation, maintenance, malignan...

Thomas, A.M.; Shea, L.D., 2014: Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury. Gene delivering biomaterials have increasingly been employed to modulate the cellular microenvironment to promote tissue regeneration, yet low transduction efficiency has been a persistent challenge for <italic>in vivo</italic> applica...

Atasheva, S.; Krendelchtchikova, V.; Liopo, A.; Frolova, E.; Frolov, I., 2010: Interplay of acute and persistent infections caused by Venezuelan equine encephalitis virus encoding mutated capsid protein. Venezuelan equine encephalitis virus (VEEV) is a significant human and animal pathogen. The highlight of VEEV replication in vitro, in cells of vertebrate origin, is the rapid development of cytopathic effect (CPE), which is strongly dependent upo...

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Platnick N.I., 1987: An empirical comparison of microcomputer parsimony programs. The effectiveness and efficiency of two microcomputer parsimony programs (Felsenstein's PHYLIP and Swofford's PAUP), and prototype version of a third (SHEN, to be incorporated in Farris' HENNIG-86), are evaluated with reference to 3...

Momiyama, T.T., 1930: List of the Birdskms from the Island of Botel Tobago made by Mrs. Z. Suzuki in June of1929. Amoeba Tokyo, 21: pp 25-28

Larenas Yáñez, G., 1990: Atriopeptin: a new hormone in water-salt regulation. The discovery of biologically active peptides in mammalian atria represents an important advance in the area of cardiovascular and renal research. Atrial natriuretic factor has been isolated from atrial cardiocytes and is released into the blood a...

Warren-Gash, C., 2014: Influenza and ischaemic heart disease: research challenges and future directions. Heart 99(24): 1795-1796

Seck Hans Adolf; Koetz Juergen; Okrusch Martin; Seidel Eberhard; Stosch Heinz Guenter, 1996: Geochemistry of a meta-ophiolite suite; an associated metagabbros, eclogites and glaucophanites on the island of Syros, Greece. European Journal of Mineralogy 8(3): 607-623